Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   AB0R72_RS13920 Genome accession   NZ_CP160229
Coordinates   2832625..2833239 (-) Length   204 a.a.
NCBI ID   WP_064107157.1    Uniprot ID   -
Organism   Bacillus velezensis strain AP46     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2827625..2838239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R72_RS13890 (AB0R72_13895) rpsT 2827928..2828194 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  AB0R72_RS13895 (AB0R72_13900) holA 2828211..2829252 (-) 1042 Protein_2694 DNA polymerase III subunit delta -
  AB0R72_RS13900 (AB0R72_13905) - 2829292..2829443 (-) 152 Protein_2695 hypothetical protein -
  AB0R72_RS13905 (AB0R72_13910) - 2829484..2829618 (+) 135 WP_003152870.1 YqzM family protein -
  AB0R72_RS13910 (AB0R72_13915) comEC 2829637..2831988 (-) 2352 WP_060674655.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  AB0R72_RS13915 (AB0R72_13920) - 2831989..2832558 (-) 570 WP_003152868.1 ComE operon protein 2 -
  AB0R72_RS13920 (AB0R72_13925) comEA 2832625..2833239 (-) 615 WP_064107157.1 helix-hairpin-helix domain-containing protein Machinery gene
  AB0R72_RS13925 (AB0R72_13930) comER 2833298..2834119 (+) 822 WP_064107158.1 late competence protein ComER -
  AB0R72_RS13930 (AB0R72_13935) - 2834188..2834925 (-) 738 WP_015417856.1 class I SAM-dependent methyltransferase -
  AB0R72_RS13935 (AB0R72_13940) rsfS 2834922..2835278 (-) 357 WP_007408260.1 ribosome silencing factor -
  AB0R72_RS13940 (AB0R72_13945) yqeK 2835296..2835856 (-) 561 WP_007408259.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  AB0R72_RS13945 (AB0R72_13950) - 2835846..2836415 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  AB0R72_RS13950 (AB0R72_13955) yhbY 2836426..2836716 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -
  AB0R72_RS13955 (AB0R72_13960) aroE 2836710..2837552 (-) 843 WP_033574150.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22249.47 Da        Isoelectric Point: 6.4888

>NTDB_id=1020209 AB0R72_RS13920 WP_064107157.1 2832625..2833239(-) (comEA) [Bacillus velezensis strain AP46]
MTKAMMTERLRRNLKILVACLAAGAIFICIWLFRSEKEEPVKQQSVLGQTASVSVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1020209 AB0R72_RS13920 WP_064107157.1 2832625..2833239(-) (comEA) [Bacillus velezensis strain AP46]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGATTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGTAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

57.653

96.078

0.554
  comEA Lactococcus lactis subsp. cremoris KW2

35.135

100

0.382
  comEA Latilactobacillus sakei subsp. sakei 23K

33.19

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae Rx1

36.493

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae D39

36.493

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae R6

36.493

100

0.377
  comEA/celA/cilE Streptococcus mitis NCTC 12261

36.493

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

36.493

100

0.377
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment