Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABX013_RS02345 Genome accession   NZ_CP160207
Coordinates   566419..568098 (-) Length   559 a.a.
NCBI ID   WP_371751717.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Amel04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 561419..573098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABX013_RS02315 (ABX013_11105) - 561749..562669 (-) 921 WP_025329858.1 ABC transporter ATP-binding protein -
  ABX013_RS02320 (ABX013_11100) yacG 562716..562913 (-) 198 WP_078475027.1 DNA gyrase inhibitor YacG -
  ABX013_RS02325 (ABX013_11095) zapD 562913..563665 (-) 753 WP_025329860.1 cell division protein ZapD -
  ABX013_RS02330 (ABX013_11085) coaE 563671..564285 (-) 615 WP_078475026.1 dephospho-CoA kinase -
  ABX013_RS02335 (ABX013_11080) pilD 564282..565133 (-) 852 WP_367449354.1 A24 family peptidase Machinery gene
  ABX013_RS02340 (ABX013_11075) pilG 565140..566372 (-) 1233 WP_084547381.1 type II secretion system F family protein Machinery gene
  ABX013_RS02345 (ABX013_11070) pilF 566419..568098 (-) 1680 WP_371751717.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABX013_RS02350 (ABX013_11065) recJ 568299..569996 (-) 1698 WP_371751718.1 single-stranded-DNA-specific exonuclease RecJ -
  ABX013_RS02355 (ABX013_11060) - 569993..570967 (-) 975 WP_369432851.1 hypothetical protein -
  ABX013_RS02360 (ABX013_11055) - 571070..571606 (-) 537 WP_371751719.1 tyrosine-protein phosphatase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61957.49 Da        Isoelectric Point: 5.7531

>NTDB_id=1018878 ABX013_RS02345 WP_371751717.1 566419..568098(-) (pilF) [Snodgrassella alvi strain Amel04]
MSLGLIRVLYKHQIIDAEKAEKLRQELEQKHPVIPALLQSGVISADKLASFLSGVFGIPYLNLEPYNQNLVPLDLINLKI
MQQYICVPLFKRGNRLFLAVSDPTSIQHYQKIATDAGCVLNLVIVRDSQLRQLLENLGQESTTLLEDMDSIGDSSAVENN
AMSADDEDGENGPIAKFVFKILGDALNSGASDIHFEFYEEMARVRFRVDGQLREVVRMPLNIRSQIASRIKVMAKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEFALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPVSDEMQRIIMNNGNEVDISNQALKEGLVDLRHAGLLKVMEGITSLDEILATTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1018878 ABX013_RS02345 WP_371751717.1 566419..568098(-) (pilF) [Snodgrassella alvi strain Amel04]
ATGAGTCTTGGACTGATCAGGGTTTTATATAAGCACCAGATTATTGACGCAGAAAAAGCAGAAAAGCTGCGTCAGGAACT
GGAACAGAAGCATCCGGTTATACCGGCACTACTACAATCCGGAGTCATCAGTGCTGATAAACTGGCCAGCTTTCTGTCCG
GTGTATTCGGTATTCCTTACCTGAATCTGGAGCCATATAACCAAAATCTGGTACCGTTGGATCTAATTAATCTGAAAATT
ATGCAGCAATACATTTGCGTGCCGCTATTCAAGCGCGGTAACCGACTGTTTTTAGCTGTATCTGATCCTACATCAATTCA
GCATTACCAGAAAATTGCCACAGATGCCGGCTGCGTACTGAATCTGGTTATAGTCCGCGATAGTCAGTTACGCCAATTAC
TGGAAAATCTTGGTCAGGAATCCACCACCCTTCTGGAAGATATGGATTCAATTGGTGACTCCAGTGCTGTAGAAAATAAC
GCCATGTCTGCAGATGACGAAGATGGCGAAAATGGACCAATTGCAAAATTTGTTTTTAAAATACTAGGTGATGCATTAAA
TAGCGGCGCATCAGATATCCATTTTGAATTTTATGAAGAAATGGCACGAGTACGCTTTCGCGTTGACGGACAGTTGCGTG
AAGTGGTACGTATGCCACTGAATATTCGCAGCCAGATCGCTTCACGTATTAAAGTGATGGCCAAACTGGATATTTCAGAA
AAACGTATTCCTCAGGACGGACGTATTCAGATTCAGTTCCGTAAAGGTGCCAAGCCAATTGATTTCCGTGTCAGCACACT
GCCCACTCTGTTTGGGGAAAAAATCGTAATGCGTATCCTGAATTCGGATGCGGCTACTCTGAATATTGAACAACTTGGTT
TTGAGCCATTTCAGAAAGAATTACTGCTTGAAGCCATCCACCGGCCATACGGTATGGTGCTGGTTACCGGCCCTACCGGT
TCCGGTAAAACCGTATCACTGTATACCTGCCTTAATATTCTTAATACCGACAGTGTTAACATTTCCACTGCTGAAGACCC
TGCGGAAATTAACCTGCCCGGTATTAATCAGGTAAATGTTAATGATAAACAGGGACTGACATTTGCTGCAGCGTTAAAAT
CATTTCTGCGCCAGGATCCGGATATCATTATGGTGGGGGAAATCCGTGATTTGGAAACCGCAGATATTGCCATTAAAGCT
GCACAAACCGGCCATATGGTATTTTCAACTCTGCATACCAATAATGCCCCTGCTACATTATCCCGTATGCTGAATATGGG
CGTTGCTCCGTTTAATATTGCCAGCTCAGTAAATCTGATTATGGCACAGCGTCTGGTGCGGCGTTTATGTACCTGCAAGG
CACCGGCAGAGCGTCCGCCTGAATTCGCACTGAAAAAAGCAGGTTTTTCAGATGAAGATCTCGCCAAAGAATGGACATTT
TATCGGCCGGTCGGCTGTGAAAGGTGTAAGGGTAAAGGCTATAAAGGACGTGCCGGCGTATATGAAGTTATGCCGGTCAG
TGATGAAATGCAACGTATTATCATGAATAATGGTAATGAAGTTGATATCAGCAATCAGGCACTCAAGGAAGGGCTAGTTG
ATTTACGTCATGCCGGTTTACTGAAAGTCATGGAAGGCATAACGTCTCTGGATGAAATTCTGGCAACCACCAATGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(180-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.036

100

0.682
  pilB Acinetobacter baylyi ADP1

51.408

100

0.522
  pilB Legionella pneumophila strain ERS1305867

49.385

100

0.503
  pilB Acinetobacter baumannii D1279779

52.336

95.707

0.501
  pilB Vibrio campbellii strain DS40M4

44.405

100

0.447
  pilB Vibrio cholerae strain A1552

44.563

100

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

43.816

100

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.678

94.991

0.386
  pilF Thermus thermophilus HB27

40.112

95.886

0.385

Multiple sequence alignment