Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABX042_RS06375 Genome accession   NZ_CP160076
Coordinates   1420626..1422305 (+) Length   559 a.a.
NCBI ID   WP_084547380.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Amel01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1415626..1427305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABX042_RS06360 (ABX042_12305) - 1417117..1417650 (+) 534 WP_025329867.1 tyrosine-protein phosphatase -
  ABX042_RS06365 (ABX042_12310) - 1417756..1418730 (+) 975 WP_367454584.1 hypothetical protein -
  ABX042_RS06370 (ABX042_12315) recJ 1418727..1420424 (+) 1698 WP_370578623.1 single-stranded-DNA-specific exonuclease RecJ -
  ABX042_RS06375 (ABX042_12320) pilF 1420626..1422305 (+) 1680 WP_084547380.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABX042_RS06380 (ABX042_12325) pilG 1422354..1423586 (+) 1233 WP_367454586.1 type II secretion system F family protein Machinery gene
  ABX042_RS06385 (ABX042_12330) pilD 1423593..1424444 (+) 852 WP_198210315.1 A24 family peptidase Machinery gene
  ABX042_RS06390 (ABX042_12335) coaE 1424441..1425055 (+) 615 WP_084551530.1 dephospho-CoA kinase -
  ABX042_RS06395 (ABX042_12340) zapD 1425061..1425813 (+) 753 WP_025329860.1 cell division protein ZapD -
  ABX042_RS06400 (ABX042_12345) yacG 1425813..1426010 (+) 198 WP_025329859.1 DNA gyrase inhibitor YacG -
  ABX042_RS06405 (ABX042_12350) - 1426057..1426977 (+) 921 WP_198210313.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61934.46 Da        Isoelectric Point: 5.7840

>NTDB_id=1017691 ABX042_RS06375 WP_084547380.1 1420626..1422305(+) (pilF) [Snodgrassella alvi strain Amel01]
MSLGLIRVLYKHQIIDAEKAEKLRQELEQKRPVIPALLQSGAINADKLASFLSGVFGIPYLNLEPYNQSLVPLDLINLKI
MQQYICVPLFKRGNRLFLAVSDPTSIQHYQKIATDAGCVLNLVIVRDSQLRQLLENLGQESTNLLEDMDSIGDSSAVENN
AMSADDEDGENGPIAKFVFKILGDALNSGASDIHFEFYEEMARVRFRVDGQLREVVRMPLNIRSQIASRIKVMAKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEFALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPVSDEMQRIIMNNGNEVDISTQALKEGLVDLRHAGLLKVMEGITSLDEILASTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1017691 ABX042_RS06375 WP_084547380.1 1420626..1422305(+) (pilF) [Snodgrassella alvi strain Amel01]
ATGAGTCTTGGACTGATCAGGGTTTTATATAAGCACCAGATTATTGACGCAGAAAAAGCAGAAAAACTGCGTCAGGAACT
GGAACAGAAACGTCCGGTTATACCGGCATTACTACAATCTGGAGCCATCAATGCTGATAAACTGGCCAGCTTTCTGTCCG
GTGTATTTGGTATTCCTTACCTGAATCTGGAGCCATATAACCAAAGTCTGGTACCGCTGGATTTAATTAATCTGAAAATA
ATGCAGCAATACATTTGCGTGCCGCTATTCAAGCGCGGTAATCGACTGTTTTTGGCTGTATCTGATCCTACATCAATTCA
GCATTACCAGAAAATTGCCACAGATGCCGGCTGCGTACTGAATCTGGTTATAGTCCGCGATAGTCAGTTACGCCAATTAC
TGGAAAATCTTGGTCAGGAATCCACCAACCTTCTGGAAGATATGGATTCAATTGGTGACTCCAGTGCTGTAGAAAATAAC
GCCATGTCTGCAGATGACGAAGATGGCGAAAATGGACCAATTGCAAAATTTGTTTTTAAAATACTTGGAGATGCATTAAA
CAGCGGCGCATCAGATATCCATTTTGAATTTTATGAAGAAATGGCACGAGTACGCTTTCGCGTTGACGGACAGTTGCGTG
AAGTGGTACGTATGCCACTGAATATTCGCAGCCAGATCGCTTCACGTATTAAAGTGATGGCCAAACTGGACATTTCTGAA
AAACGTATTCCTCAGGACGGACGTATTCAGATTCAGTTCCGTAAAGGTGCCAAGCCAATTGATTTCCGTGTCAGCACACT
GCCCACTCTGTTCGGGGAAAAAATTGTAATGCGTATCCTGAATTCGGATGCGGCTACTCTGAATATTGAACAACTTGGTT
TTGAGCCATTTCAGAAAGAATTACTGCTTGAGGCCATCCACCGGCCATATGGTATGGTGCTGGTTACTGGCCCTACCGGT
TCCGGTAAAACCGTATCACTGTATACCTGCCTTAATATTCTTAATACCGACAGTGTTAACATTTCCACTGCTGAAGACCC
TGCGGAAATTAACCTGCCTGGTATTAACCAGGTAAATGTTAATGATAAACAAGGACTGACATTTGCTGCAGCGTTAAAAT
CATTTCTGCGCCAGGATCCGGATATCATTATGGTGGGGGAAATCCGTGATTTGGAAACCGCAGATATTGCCATTAAAGCT
GCACAAACCGGCCATATGGTATTTTCAACTCTGCATACCAATAATGCCCCTGCTACATTATCCCGTATGCTGAATATGGG
CGTTGCGCCGTTTAATATTGCCAGCTCAGTAAATCTGATTATGGCACAGCGTCTGGTGCGGCGTTTATGTACCTGCAAGG
CACCGGCAGAGCGTCCGCCTGAATTTGCACTGAAAAAAGCAGGTTTTTCAGATGAAGATCTCGCCAAAGAATGGACATTT
TACCGGCCGGTCGGCTGTGAAAGGTGTAAGGGCAAAGGCTATAAAGGACGTGCCGGCGTATATGAAGTTATGCCGGTCAG
TGATGAAATGCAACGTATTATCATGAATAATGGTAATGAAGTAGATATCAGCACTCAGGCGCTCAAGGAAGGGCTAGTTG
ATTTACGCCATGCCGGTTTACTGAAAGTCATGGAAGGCATTACGTCTCTGGACGAGATTCTTGCTAGCACTAATGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(180-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

67.679

100

0.678
  pilB Acinetobacter baylyi ADP1

51.601

100

0.519
  pilB Legionella pneumophila strain ERS1305867

49.209

100

0.501
  pilB Acinetobacter baumannii D1279779

52.741

94.633

0.499
  pilB Vibrio cholerae strain A1552

44.92

100

0.451
  pilB Vibrio campbellii strain DS40M4

44.405

100

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

43.816

100

0.444
  pilF Thermus thermophilus HB27

40.112

95.886

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.152

94.454

0.379

Multiple sequence alignment