Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABX026_RS00715 Genome accession   NZ_CP160075
Coordinates   147956..149635 (+) Length   559 a.a.
NCBI ID   WP_367424191.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Amel10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 142956..154635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABX026_RS00700 (ABX026_15195) - 144447..144980 (+) 534 WP_025329867.1 tyrosine-protein phosphatase -
  ABX026_RS00705 (ABX026_15200) - 145086..146060 (+) 975 WP_371751035.1 hypothetical protein -
  ABX026_RS00710 (ABX026_09585) recJ 146057..147754 (+) 1698 WP_371751036.1 single-stranded-DNA-specific exonuclease RecJ -
  ABX026_RS00715 (ABX026_09590) pilF 147956..149635 (+) 1680 WP_367424191.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABX026_RS00720 (ABX026_09595) pilG 149684..150916 (+) 1233 WP_025329863.1 type II secretion system F family protein Machinery gene
  ABX026_RS00725 (ABX026_09600) pilD 150923..151774 (+) 852 WP_174829707.1 A24 family peptidase Machinery gene
  ABX026_RS00730 (ABX026_09605) coaE 151771..152385 (+) 615 WP_367424195.1 dephospho-CoA kinase -
  ABX026_RS00735 (ABX026_09610) zapD 152391..153143 (+) 753 WP_279027999.1 cell division protein ZapD -
  ABX026_RS00740 (ABX026_09615) yacG 153143..153340 (+) 198 WP_174829705.1 DNA gyrase inhibitor YacG -
  ABX026_RS00745 (ABX026_09620) - 153387..154307 (+) 921 WP_025329858.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61893.40 Da        Isoelectric Point: 5.7840

>NTDB_id=1017655 ABX026_RS00715 WP_367424191.1 147956..149635(+) (pilF) [Snodgrassella alvi strain Amel10]
MSLGLIRVLYKHQIIDAEKAEKLRQELEQKRPVIPALLQSGAISADKLASFLSGVFGIPYLNLEPYNQSLVPLDLINLKI
MQQYICVPLFKRGNRLFLAVSDPTSIQHYQKIATDAGCVLNLVIVRDSQLRQLLENLGQESTNLLEDMDSIGDSSAVENN
AMSADDEDGENGPIAKFVFKILGDALNSGASDIHFEFYEEMARVRFRVDGQLREVVRMPLNIRSQIASRIKVMAKLDISE
KRIPQDGRIQVQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEFALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPVSDEMQRIIMNNGNEVDISTQALKEGLVDLRHAGLLKVMEGITSLDEILASTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1017655 ABX026_RS00715 WP_367424191.1 147956..149635(+) (pilF) [Snodgrassella alvi strain Amel10]
ATGAGTCTTGGACTGATCAGGGTTTTATATAAACACCAGATTATTGACGCAGAAAAAGCAGAAAAACTGCGTCAGGAACT
GGAACAGAAACGTCCGGTTATACCGGCACTACTACAATCTGGAGCCATCAGTGCTGATAAACTGGCCAGCTTTCTGTCCG
GTGTATTTGGTATTCCTTACCTGAATCTGGAGCCATATAACCAAAGTCTGGTACCGCTGGATTTAATTAATCTGAAAATT
ATGCAGCAATACATTTGCGTGCCGCTATTCAAGCGCGGTAACCGACTGTTTTTGGCTGTATCTGATCCTACATCAATTCA
GCATTACCAGAAAATTGCCACAGATGCCGGCTGTGTACTGAATCTGGTTATAGTCCGCGATAGTCAGTTACGCCAATTAC
TGGAAAATCTTGGTCAGGAATCCACCAACCTTCTGGAAGATATGGATTCAATTGGTGACTCCAGTGCTGTAGAAAATAAC
GCCATGTCTGCAGATGACGAAGATGGCGAAAATGGACCAATTGCAAAATTTGTTTTTAAAATACTTGGAGATGCATTAAA
CAGCGGCGCATCAGATATCCATTTTGAATTTTATGAAGAAATGGCACGAGTACGTTTTCGCGTTGACGGACAGTTGCGTG
AAGTGGTACGTATGCCACTGAATATTCGCAGCCAGATCGCTTCACGTATTAAAGTGATGGCCAAACTGGACATTTCAGAA
AAACGTATTCCTCAGGACGGACGTATTCAGGTTCAGTTCCGTAAAGGTGCCAAGCCGATTGATTTCCGTGTCAGCACACT
GCCCACTCTGTTCGGGGAAAAAATTGTAATGCGTATCCTGAATTCGGATGCGGCTACTCTGAATATTGAACAACTTGGTT
TTGAGCCATTTCAGAAAGAATTACTGCTTGAGGCCATCCACCGGCCATACGGTATGGTGCTGGTTACCGGCCCTACTGGT
TCCGGTAAAACCGTATCACTGTATACCTGCCTTAATATTCTTAATACCGACAGTGTTAACATTTCCACTGCTGAAGACCC
TGCGGAAATTAACCTGCCTGGTATTAATCAGGTAAATGTTAATGATAAACAAGGACTGACATTTGCTGCTGCGTTAAAAT
CATTTCTGCGCCAGGATCCGGATATCATTATGGTGGGGGAAATCCGTGATTTGGAAACCGCAGATATTGCCATTAAAGCT
GCACAAACCGGCCATATGGTATTTTCAACTCTGCATACCAATAATGCCCCTGCTACATTATCCCGTATGCTGAATATGGG
CGTTGCTCCGTTTAATATTGCCAGCTCAGTAAATCTGATTATGGCACAGCGTCTGGTGCGGCGTTTATGTACCTGCAAGG
CACCGGCAGAGCGTCCGCCTGAATTTGCACTGAAAAAAGCAGGTTTTTCAGATGAAGATCTCGCCAAAGAATGGACATTT
TACCGGCCGGTCGGCTGTGAAAGGTGTAAGGGCAAAGGCTATAAAGGACGTGCCGGCGTATATGAAGTTATGCCGGTCAG
TGATGAAATGCAACGTATTATCATGAATAATGGTAATGAAGTAGATATCAGCACTCAGGCGCTCAAGGAAGGTCTGGTCG
ATTTACGCCATGCCGGTTTACTGAAAGTGATGGAAGGCATTACGTCTCTGGACGAGATTCTTGCAAGCACTAATGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(180-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

67.857

100

0.68
  pilB Acinetobacter baylyi ADP1

52.135

100

0.524
  pilB Legionella pneumophila strain ERS1305867

49.385

100

0.503
  pilB Acinetobacter baumannii D1279779

52.93

94.633

0.501
  pilB Vibrio cholerae strain A1552

44.92

100

0.451
  pilB Vibrio campbellii strain DS40M4

44.405

100

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

43.816

100

0.444
  pilF Thermus thermophilus HB27

40.299

95.886

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.53

94.454

0.383

Multiple sequence alignment