Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABX014_RS03935 Genome accession   NZ_CP160070
Coordinates   848744..850423 (+) Length   559 a.a.
NCBI ID   WP_370578343.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Amel05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 843744..855423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABX014_RS03920 (ABX014_20605) - 845233..845769 (+) 537 WP_174832879.1 tyrosine-protein phosphatase -
  ABX014_RS03925 (ABX014_13950) - 845876..846850 (+) 975 WP_370578342.1 hypothetical protein -
  ABX014_RS03930 (ABX014_13945) recJ 846847..848544 (+) 1698 WP_367435912.1 single-stranded-DNA-specific exonuclease RecJ -
  ABX014_RS03935 (ABX014_13940) pilF 848744..850423 (+) 1680 WP_370578343.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABX014_RS03940 (ABX014_13935) pilG 850472..851704 (+) 1233 WP_037476130.1 type II secretion system F family protein Machinery gene
  ABX014_RS03945 (ABX014_13930) pilD 851711..852562 (+) 852 WP_370578344.1 A24 family peptidase Machinery gene
  ABX014_RS03950 (ABX014_20610) coaE 852559..853173 (+) 615 WP_370578345.1 dephospho-CoA kinase -
  ABX014_RS03955 (ABX014_20615) zapD 853179..853931 (+) 753 WP_037476209.1 cell division protein ZapD -
  ABX014_RS03960 (ABX014_13900) yacG 853931..854128 (+) 198 WP_025329859.1 DNA gyrase inhibitor YacG -
  ABX014_RS03965 (ABX014_13895) - 854175..855095 (+) 921 WP_037476124.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61999.39 Da        Isoelectric Point: 5.3036

>NTDB_id=1017535 ABX014_RS03935 WP_370578343.1 848744..850423(+) (pilF) [Snodgrassella alvi strain Amel05]
MSVGLIRVLYKHQIIDAEKAEKLRQELEQKHPVIPALLQSGAIDADKLASFLSSVFSIPYLNLELYNQSLVPMDLIDLNI
MQQYICVPIFKRSNRMFLAVSDPTSIQHYQKIATDAGCVLNLVIVRDDQLRLLLENISQESTSLLEGMDSVGDSTAAENN
AMSADDEEGENGPVARFVFKVLGDALNSGASDIHFEFYEEMARVRFRVDGQLREVVRMPLNIRNQIASRIKVMSKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEQALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPVSDEMQRIIMNNGNEVDISTQALKEGLVDLRHAGLLKVMEGITSLDEILASTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1017535 ABX014_RS03935 WP_370578343.1 848744..850423(+) (pilF) [Snodgrassella alvi strain Amel05]
ATGAGTGTAGGATTGATTCGGGTTTTATATAAGCACCAGATTATTGATGCTGAGAAAGCAGAAAAACTGCGTCAGGAATT
AGAGCAGAAACACCCGGTAATACCGGCTCTGTTACAATCCGGAGCTATTGATGCCGATAAACTGGCCAGTTTTCTGTCCA
GTGTATTCAGTATTCCTTACCTGAATCTGGAGCTATATAATCAAAGTCTGGTTCCTATGGATTTAATTGATTTGAATATT
ATGCAGCAATATATTTGCGTGCCGATATTCAAACGCAGTAATCGCATGTTTCTGGCCGTATCCGATCCTACATCAATTCA
GCATTATCAAAAAATTGCCACTGATGCCGGCTGTGTACTGAATCTGGTTATTGTCCGCGACGACCAGCTACGCCTGCTAC
TGGAGAATATTAGTCAGGAATCTACCAGCCTTCTTGAGGGTATGGATTCAGTTGGCGACTCGACTGCAGCAGAAAATAAC
GCTATGTCTGCCGACGATGAAGAAGGCGAAAACGGACCGGTCGCCAGATTTGTCTTTAAAGTACTGGGAGATGCTTTAAA
TAGTGGCGCATCAGATATTCATTTTGAATTTTACGAAGAAATGGCACGTGTTCGCTTCCGTGTTGACGGACAGTTGCGTG
AAGTTGTACGTATGCCACTGAATATCCGTAATCAGATTGCTTCACGTATTAAGGTAATGTCCAAACTGGATATTTCAGAA
AAACGCATCCCTCAGGACGGGCGTATTCAGATTCAGTTCCGTAAAGGTGCCAAGCCGATTGATTTCCGTGTCAGCACACT
GCCTACTCTGTTTGGTGAAAAAATTGTAATGCGTATCCTGAATTCGGATGCGGCTACTCTGAATATCGAACAGCTTGGTT
TTGAACCATTTCAGAAAGAATTACTGCTCGAAGCTATCCACCGGCCGTATGGTATGGTGCTGGTTACCGGCCCTACTGGT
TCCGGTAAAACCGTATCACTGTATACCTGCCTTAATATTCTTAATACTGACAGTGTTAATATTTCCACTGCTGAAGATCC
GGCAGAAATTAACCTGCCCGGTATTAATCAGGTAAATGTTAATGATAAACAGGGACTGACATTCGCTGCAGCATTAAAAT
CGTTTCTGCGTCAGGATCCGGATATCATTATGGTGGGGGAAATCCGGGATCTGGAAACAGCTGATATTGCCATTAAGGCT
GCACAAACCGGTCATATGGTATTTTCAACCCTGCATACCAATAATGCCCCGGCTACTTTATCCCGTATGCTGAATATGGG
TGTCGCACCATTCAATATTGCCAGCTCTGTTAATCTGATTATGGCACAGCGTCTGGTACGGCGTTTATGCACCTGCAAAG
CTCCGGCAGAACGCCCGCCTGAACAAGCATTAAAAAAAGCTGGTTTTTCAGATGAAGATCTGGCTAAGGAATGGACATTT
TACCGGCCAGTCGGCTGTGAGAGATGTAAAGGTAAAGGTTATAAAGGACGTGCCGGCGTGTACGAAGTTATGCCGGTCAG
CGATGAAATGCAACGTATTATCATGAATAATGGTAATGAAGTTGATATCAGCACTCAGGCGCTCAAGGAAGGTCTGGTCG
ATTTACGTCATGCCGGTTTACTGAAAGTCATGGAAGGCATTACGTCTCTGGACGAGATTCTTGCAAGCACTAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(180-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.929

100

0.691
  pilB Acinetobacter baylyi ADP1

51.068

100

0.513
  pilB Acinetobacter baumannii D1279779

52.354

94.991

0.497
  pilB Legionella pneumophila strain ERS1305867

47.78

100

0.481
  pilB Vibrio cholerae strain A1552

44.92

100

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

43.993

100

0.445
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.662

95.17

0.377
  pilF Thermus thermophilus HB27

39.736

94.991

0.377

Multiple sequence alignment