Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABU614_RS11690 Genome accession   NZ_CP159925
Coordinates   2698190..2699914 (-) Length   574 a.a.
NCBI ID   WP_363796232.1    Uniprot ID   A0AAU8MNE2
Organism   Lysobacter firmicutimachus strain SR10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2693190..2704914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU614_RS11670 (ABU614_11670) - 2693904..2694857 (+) 954 WP_363800847.1 Nudix family hydrolase -
  ABU614_RS11675 (ABU614_11675) coaE 2695387..2696001 (-) 615 WP_363796230.1 dephospho-CoA kinase -
  ABU614_RS11680 (ABU614_11680) - 2696006..2696893 (-) 888 WP_064746435.1 A24 family peptidase -
  ABU614_RS11685 (ABU614_11685) pilC 2696915..2698186 (-) 1272 WP_363796231.1 type II secretion system F family protein Machinery gene
  ABU614_RS11690 (ABU614_11690) pilB 2698190..2699914 (-) 1725 WP_363796232.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABU614_RS11695 (ABU614_11695) - 2700049..2700900 (+) 852 WP_363796233.1 glycosyltransferase family 2 protein -
  ABU614_RS11700 (ABU614_11700) - 2700915..2702021 (+) 1107 WP_064746438.1 UDP-N-acetylglucosamine 2-epimerase -
  ABU614_RS11705 (ABU614_11705) - 2702012..2702857 (+) 846 WP_363796234.1 hypothetical protein -
  ABU614_RS11710 (ABU614_11710) - 2702854..2703534 (+) 681 WP_141233329.1 hypothetical protein -
  ABU614_RS11715 (ABU614_11715) - 2703531..2704586 (+) 1056 WP_141233330.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62709.09 Da        Isoelectric Point: 6.1041

>NTDB_id=1016820 ABU614_RS11690 WP_363796232.1 2698190..2699914(-) (pilB) [Lysobacter firmicutimachus strain SR10]
MSIATANLVGITGIARRLVMDGALDEVKAREAMTAASADRKPIATYLAEQRLVTSGQLAAANSIEFGVPLFDPSAMDPSQ
SAVRAISEELIRKHNALPLFKRGNKLFIGLADPTNTGALDEIKFQTNAAVEAILVDEELIRRTLERWLESSDNLATTVGD
GEGLETLEIGKDEDIANVGDSGIDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQVAKVPNKL
HARIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDDQKELFV
GAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDEQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDVI
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHAL
LAEGFSHEEIASGFKLYEPAGCPDCTEGYKGRTGIYQVMPMTEEIQAIVLEGGNAMQIAEAAQRSGVRDLRRSALIKVMN
GVTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1016820 ABU614_RS11690 WP_363796232.1 2698190..2699914(-) (pilB) [Lysobacter firmicutimachus strain SR10]
ATGTCCATCGCAACTGCCAATCTGGTGGGAATCACCGGCATTGCCCGGCGCTTGGTCATGGACGGCGCCCTGGACGAGGT
CAAGGCGCGCGAGGCCATGACCGCGGCCAGCGCCGACCGCAAACCGATCGCCACGTATCTGGCCGAACAACGCCTGGTCA
CGTCGGGGCAACTGGCGGCCGCCAACTCGATCGAGTTCGGCGTGCCCCTGTTCGACCCTTCGGCCATGGACCCGAGCCAG
TCGGCCGTGCGCGCGATCAGCGAAGAACTGATCCGCAAGCACAACGCCCTGCCACTGTTCAAGCGCGGCAACAAGCTGTT
CATCGGCCTGGCCGATCCGACCAATACCGGCGCCCTGGACGAGATCAAGTTCCAGACCAACGCCGCGGTCGAGGCCATCC
TGGTCGACGAGGAGCTGATCCGCCGCACCCTGGAGCGCTGGCTGGAGAGTTCCGACAACCTCGCCACCACGGTCGGCGAC
GGCGAGGGCCTGGAAACCCTCGAGATCGGCAAGGACGAAGACATCGCCAACGTCGGCGACAGCGGCATCGACGCCAAGGG
CGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGACGCGATCCGCCGGGGCGCCTCGGACATCCACTTCG
AGCCTTACGAGACCGAGTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCAAGGTGCCGAACAAGCTG
CACGCGCGCATCGCCGCGCGCCTGAAGGTCATGGCCCAGCTCGACATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCAT
CAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCTTGTTCGGCGAGAAGATCGTGC
TGCGTATCCTCGACGGCAGCGCGGCCCGGCTGGGCATCGAGAAGCTCGGCTACGAGGACGACCAGAAGGAATTGTTCGTC
GGCGCGGTGGTCAAGCCCTACGGCATGGTCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTACACCGC
GCTCAACATCCTCAACGACGAGCAACGCAACATCTCCACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCAACC
AGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCGGCCCTGCGCAGCTTCCTGCGCCAGGACCCGGACGTGATC
ATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTGCTGTCGAC
CCTGCACACCAACGACGCCCCGCAGACCATCGCCCGACTGATGAACATGGGCGTGGCGCCGTACAACATCACTTCCTCGG
TCACTCTGGTGATCGCCCAGCGCCTCGCCCGCCGTCTGCACGACTGCAAGCGCGAGGTGCACCTGCCGGAACACGCGCTG
CTCGCCGAAGGCTTCAGCCACGAAGAGATCGCTTCCGGCTTCAAGTTGTACGAGCCGGCGGGATGCCCGGACTGCACCGA
AGGCTACAAAGGACGTACCGGCATCTACCAGGTCATGCCGATGACCGAAGAAATCCAGGCGATCGTGCTCGAGGGCGGCA
ATGCGATGCAGATCGCCGAAGCCGCCCAGCGCTCGGGCGTGCGCGACCTGCGCCGCTCGGCACTGATCAAGGTGATGAAC
GGCGTCACCAGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.386

99.303

0.54
  pilB Acinetobacter baumannii D1279779

54.577

98.955

0.54
  pilB Legionella pneumophila strain ERS1305867

50.088

99.477

0.498
  pilB Vibrio cholerae strain A1552

48.696

100

0.488
  pilF Neisseria gonorrhoeae MS11

46.842

99.303

0.465
  pilB Vibrio campbellii strain DS40M4

49.341

92.509

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

49.527

92.16

0.456
  pilF Thermus thermophilus HB27

39.777

93.728

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.252

93.206

0.366

Multiple sequence alignment