Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABXT62_RS04070 Genome accession   NZ_CP159826
Coordinates   866084..867805 (-) Length   573 a.a.
NCBI ID   WP_415357266.1    Uniprot ID   -
Organism   Halioglobus sp. Uisw_031     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 861084..872805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXT62_RS04045 (ABXT62_04050) argJ 861691..862917 (+) 1227 WP_415357261.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  ABXT62_RS04050 (ABXT62_04055) mutT 862918..863316 (+) 399 WP_415357262.1 8-oxo-dGTP diphosphatase MutT -
  ABXT62_RS04055 (ABXT62_04060) coaE 863326..863931 (-) 606 WP_415357263.1 dephospho-CoA kinase -
  ABXT62_RS04060 (ABXT62_04065) pilD 863933..864796 (-) 864 WP_415357264.1 prepilin peptidase Machinery gene
  ABXT62_RS04065 (ABXT62_04070) pilC 864823..866043 (-) 1221 WP_415357265.1 type II secretion system F family protein Machinery gene
  ABXT62_RS04070 (ABXT62_04075) pilB 866084..867805 (-) 1722 WP_415357266.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABXT62_RS04075 (ABXT62_04080) - 868230..868739 (+) 510 WP_415357267.1 pilin -
  ABXT62_RS04080 (ABXT62_04085) - 868848..871025 (-) 2178 WP_415357268.1 malate synthase G -
  ABXT62_RS04085 (ABXT62_04090) - 871158..872114 (-) 957 WP_415357269.1 LysR family transcriptional regulator -
  ABXT62_RS04090 (ABXT62_04095) rraA 872292..872774 (+) 483 WP_415358161.1 ribonuclease E activity regulator RraA -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62238.05 Da        Isoelectric Point: 4.9363

>NTDB_id=1016066 ABXT62_RS04070 WP_415357266.1 866084..867805(-) (pilB) [Halioglobus sp. Uisw_031]
MNSKALGQLSGLAGSLVAGSLITAEQAVEAQKQAAIEKVHFVQYLVEKENVDGRRLAELTSHEFGLPLFDIAALNTDSIS
VALVDAALMQKHHALPLLHHGNRLFVAVSDPTNLAALAEIKFHTGINTDAVLVEESALSEALAKCAESQDALSDGLSDLD
ASYLDDIDIVSDGEAKEEDDGSESIDETPIVRFVNKVLIDAIKQGASDIHFEPYEHSYRVRFRTDGILEEVVKPPRNLAP
RLAARLKVMSQMDISERRLPQDGRIQMKLSRNRAIDFRVNTLPTLYGEKIVLRILDPGSAKMGIDALGYESYQKDMYLKV
LNQPQGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLDGVNQVHVNAKVGLNFAEALRSFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHLVLSTLHTNSASETITRLLNMGVPAFNVASSVNLIIAQRLARRLCKTCSVPADDIPHGTL
LEEGFTEDMLTGATIMSPVGCKDCNAGYKGRVGIYEVVKITPSIAKLIMEEGNSMQIATQARAEGFHDLRIAALKKVAQG
MTSLEEANRITVD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=1016066 ABXT62_RS04070 WP_415357266.1 866084..867805(-) (pilB) [Halioglobus sp. Uisw_031]
ATGAACAGTAAAGCACTGGGGCAGCTCAGCGGTTTGGCGGGAAGCCTTGTGGCTGGATCGCTGATCACCGCCGAGCAGGC
CGTCGAAGCGCAAAAACAAGCGGCCATAGAGAAGGTGCACTTTGTCCAGTATCTGGTAGAGAAGGAAAACGTCGACGGTC
GACGTCTGGCAGAGCTGACCTCACACGAATTCGGCTTACCCTTATTTGATATAGCCGCGCTCAATACCGATTCGATTTCC
GTTGCCCTGGTCGATGCAGCGTTGATGCAAAAGCACCATGCGCTGCCGCTGTTACACCACGGCAATCGGCTCTTTGTCGC
GGTATCAGACCCGACCAATCTAGCCGCCCTTGCAGAGATAAAATTTCACACTGGCATCAACACGGATGCCGTGCTGGTGG
AAGAAAGCGCCCTCAGTGAGGCACTGGCTAAATGTGCCGAGTCACAGGACGCACTCTCCGATGGGCTGAGTGATCTGGAC
GCGTCCTATCTAGACGATATTGATATCGTCAGCGACGGAGAAGCGAAAGAAGAGGATGATGGCAGCGAGTCGATTGACGA
AACACCCATTGTGCGGTTTGTTAATAAAGTCTTAATAGACGCGATTAAACAGGGTGCGTCAGACATCCACTTTGAACCTT
ATGAACATTCCTATCGGGTGCGCTTTCGCACCGATGGCATTCTGGAGGAAGTGGTTAAACCTCCGCGAAATCTTGCCCCA
CGCCTCGCGGCGCGGCTAAAGGTCATGTCGCAAATGGATATTTCAGAACGGCGGCTACCTCAGGACGGACGTATCCAGAT
GAAGCTGTCGCGCAATCGGGCTATCGATTTTCGCGTCAATACACTGCCCACCCTGTACGGCGAAAAAATCGTACTGCGTA
TTCTGGATCCTGGCAGCGCAAAAATGGGCATCGACGCCCTGGGCTATGAGTCCTACCAAAAAGACATGTACCTAAAAGTA
CTGAACCAGCCCCAGGGCATGATTCTGGTCACAGGCCCCACAGGCTCGGGAAAAACAGTGTCGCTCTATACTGGCCTCAA
TATTCTCAACACGCCCGAGCGTAATATTTCCACCGCTGAGGACCCGGTGGAAATTAATCTCGATGGCGTCAACCAGGTTC
ATGTGAATGCTAAGGTAGGACTTAATTTTGCGGAGGCATTGCGCTCCTTCCTGCGCCAGGATCCAGACGTGATCATGGTG
GGTGAGATTCGAGACCTCGAAACAGCAGAAATCGCGATCAAAGCCGCACAAACAGGCCACCTAGTGCTTTCTACGCTACA
CACCAACAGTGCCTCGGAAACCATTACCCGGTTGCTCAATATGGGAGTCCCGGCGTTTAACGTAGCGTCTTCGGTCAACC
TGATCATAGCGCAGCGACTGGCCCGGCGATTGTGCAAGACCTGCAGTGTGCCAGCGGATGACATCCCCCATGGCACACTG
CTCGAAGAGGGCTTCACCGAAGACATGTTGACCGGAGCCACTATCATGAGCCCTGTGGGCTGCAAAGACTGTAATGCCGG
TTACAAGGGACGAGTGGGTATTTACGAGGTGGTTAAAATAACCCCCTCTATTGCCAAGCTTATTATGGAGGAAGGCAACT
CAATGCAAATCGCCACTCAGGCCAGAGCAGAAGGCTTTCATGATTTACGGATCGCGGCACTGAAAAAAGTTGCCCAGGGA
ATGACCAGTTTAGAAGAAGCAAACCGGATTACCGTCGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.066

97.208

0.574
  pilB Acinetobacter baylyi ADP1

56.79

98.953

0.562
  pilB Legionella pneumophila strain ERS1305867

53.94

99.651

0.538
  pilB Vibrio cholerae strain A1552

51.845

99.302

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

49.466

98.08

0.485
  pilB Vibrio campbellii strain DS40M4

48.673

98.604

0.48
  pilF Neisseria gonorrhoeae MS11

46.82

98.778

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

38.026

100

0.41

Multiple sequence alignment