Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABV520_RS08835 Genome accession   NZ_CP159584
Coordinates   1971577..1973256 (+) Length   559 a.a.
NCBI ID   WP_370386595.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Acer03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1966577..1978256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABV520_RS08820 (ABV520_17335) - 1966896..1968662 (+) 1767 WP_370386592.1 M61 family metallopeptidase -
  ABV520_RS08825 (ABV520_17340) - 1968710..1969684 (+) 975 WP_370386593.1 hypothetical protein -
  ABV520_RS08830 (ABV520_07830) recJ 1969681..1971378 (+) 1698 WP_370386594.1 single-stranded-DNA-specific exonuclease RecJ -
  ABV520_RS08835 (ABV520_07835) pilF 1971577..1973256 (+) 1680 WP_370386595.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABV520_RS08840 (ABV520_07840) pilG 1973303..1974535 (+) 1233 WP_100156468.1 type II secretion system F family protein Machinery gene
  ABV520_RS08845 (ABV520_07845) pilD 1974542..1975393 (+) 852 WP_370386596.1 A24 family peptidase Machinery gene
  ABV520_RS08850 (ABV520_07850) coaE 1975390..1976004 (+) 615 WP_367431324.1 dephospho-CoA kinase -
  ABV520_RS08855 (ABV520_07855) zapD 1976010..1976762 (+) 753 WP_100140881.1 cell division protein ZapD -
  ABV520_RS08860 (ABV520_07860) yacG 1976762..1976959 (+) 198 WP_100140841.1 DNA gyrase inhibitor YacG -
  ABV520_RS08865 (ABV520_07865) - 1977009..1977926 (+) 918 WP_370386597.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62231.79 Da        Isoelectric Point: 5.9498

>NTDB_id=1014697 ABV520_RS08835 WP_370386595.1 1971577..1973256(+) (pilF) [Snodgrassella alvi strain Acer03]
MSVGLIRVLYKHQIIDAEKAEKLRQELEQKRPVIPMLLQSGVIDADKLASFLSKVFSIPYLNLELYNQSLVPQDLINLKI
MQQYICVPIFKRGNRLFLAVSDPTSIQHYQKIATDAGCILNLVIVRDDQLRQILDNLGQESTSLLEGMDTVGDAEATGNN
AMSADDEEGENGPVARFVFKVLNDALNSGASDIHFEFYEEMARVRFRIDGQLREVVRMPLNIRNQIASRIKVMAKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEFALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPISDEMQRIIMNNGNEVDISQQAHKEGLVDLRHAGLLKVMEGITSLDEILATTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1014697 ABV520_RS08835 WP_370386595.1 1971577..1973256(+) (pilF) [Snodgrassella alvi strain Acer03]
ATGAGTGTAGGATTGATTCGAGTTTTATATAAGCACCAGATTATCGATGCAGAAAAAGCTGAAAAACTGCGTCAGGAGCT
TGAGCAGAAACGTCCAGTTATACCGATGCTGTTGCAGTCTGGTGTCATCGATGCTGACAAACTGGCCAGCTTCCTGTCCA
AGGTATTCAGTATTCCTTACCTCAATCTGGAACTATATAACCAAAGCCTTGTACCGCAGGACTTAATCAATCTGAAAATC
ATGCAGCAATATATCTGCGTGCCCATTTTCAAACGTGGAAATCGTCTGTTTCTGGCTGTATCTGACCCCACTTCCATTCA
GCATTATCAGAAAATTGCCACAGATGCCGGCTGCATACTGAATCTGGTCATTGTACGCGATGATCAGCTGCGTCAGATAC
TAGATAATCTCGGACAGGAATCCACCAGCCTACTAGAAGGAATGGACACAGTTGGTGATGCTGAAGCAACAGGCAATAAT
GCCATGTCTGCCGATGATGAGGAGGGTGAAAACGGCCCTGTGGCCAGATTTGTATTCAAAGTTTTGAATGATGCACTCAA
CAGCGGCGCCTCAGATATTCACTTTGAATTTTACGAAGAAATGGCACGAGTACGTTTTCGTATTGACGGGCAGCTACGGG
AAGTAGTGCGTATGCCACTGAATATTAGAAACCAGATTGCCTCCCGTATTAAAGTGATGGCTAAACTGGACATTTCAGAA
AAACGTATACCTCAGGATGGCCGAATTCAGATTCAATTCCGCAAAGGCGCCAAACCGATTGATTTTCGTGTCAGCACCCT
TCCGACCCTGTTTGGTGAAAAAATTGTGATGCGTATTCTGAATTCGGATGCCGCTACCCTGAATATCGAACAGCTCGGCT
TCGAACCATTTCAGAAAGAATTACTGCTGGAAGCCATTCACCGGCCGTATGGTATGGTACTGGTTACCGGTCCAACCGGT
TCCGGTAAAACTGTATCCCTTTACACCTGCCTGAATATTCTGAATACCGACAGTGTGAACATTTCTACTGCAGAAGATCC
TGCGGAAATTAATTTACCCGGTATCAATCAGGTCAATGTAAATGATAAACAGGGACTGACATTTGCCGCAGCTCTAAAAT
CTTTCCTACGTCAGGATCCAGACATCATCATGGTGGGGGAAATCCGAGATTTAGAAACGGCCGATATTGCCATCAAAGCA
GCACAAACCGGTCATATGGTATTTTCTACCCTGCACACCAATAATGCGCCTGCCACCTTATCACGTATGCTGAATATGGG
GGTTGCTCCATTCAACATAGCAAGTTCAGTGAATCTGATTATGGCACAGCGTTTGGTTCGACGTTTATGTACCTGCAAAG
CGCCAGCCGAACGGCCACCAGAATTCGCACTCAAAAAAGCCGGTTTCTCTGATGAAGATCTAGCTAAAGAATGGACATTT
TATCGGCCGGTAGGTTGTGAGCGCTGTAAAGGCAAAGGTTACAAGGGGCGCGCTGGTGTTTATGAAGTAATGCCTATCAG
TGATGAAATGCAGCGAATCATCATGAACAATGGTAATGAAGTCGATATCAGTCAGCAGGCGCATAAAGAAGGACTGGTCG
ATTTGCGTCATGCTGGTCTGCTTAAAGTTATGGAAGGCATCACTTCGCTTGACGAAATTCTAGCCACCACCAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(180-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.286

100

0.694
  pilB Acinetobacter baylyi ADP1

50.528

100

0.513
  pilB Acinetobacter baumannii D1279779

51.679

95.886

0.496
  pilB Legionella pneumophila strain ERS1305867

48.155

100

0.49
  pilB Vibrio cholerae strain A1552

45.76

100

0.463
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

43.262

100

0.436
  pilF Thermus thermophilus HB27

40.335

96.243

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.414

95.17

0.385

Multiple sequence alignment