Detailed information NTG1013815

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ABVR74_RS02975
• Genome accession: NZ_CP159484
• Coordinates: 565099..565746 (+)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain CBA3648
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 560099..570746

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABVR74_RS02950 (ABVR74_02950)	-	560228..561406 (+)	1179	WP_012898345.1	SLC13 family permease	-
ABVR74_RS02955 (ABVR74_02955)	-	561579..562142 (+)	564	WP_251900925.1	GNAT family N-acetyltransferase	-
ABVR74_RS02960 (ABVR74_02960)	-	562246..562644 (+)	399	WP_023188528.1	hypothetical protein	-
ABVR74_RS02965 (ABVR74_02965)	-	562749..563681 (+)	933	WP_353891771.1	ABC transporter ATP-binding protein	-
ABVR74_RS02970 (ABVR74_02970)	-	563678..565039 (+)	1362	WP_313791934.1	ABC transporter permease	-
ABVR74_RS02975 (ABVR74_02975)	comEA	565099..565746 (+)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
ABVR74_RS02980 (ABVR74_02980)	comEC	565727..567937 (+)	2211	WP_353891772.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABVR74_RS02985 (ABVR74_02985)	-	568222..568998 (+)	777	WP_023188533.1	alpha/beta hydrolase family protein	-
ABVR74_RS02990 (ABVR74_02990)	-	569183..569398 (+)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
ABVR74_RS02995 (ABVR74_02995)	atpB	569443..570156 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
ABVR74_RS03000 (ABVR74_03000)	atpF	570171..570677 (+)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=1013815 ABVR74_RS02975 WP_010906131.1 565099..565746(+) (comEA) [Lactococcus lactis subsp. lactis strain CBA3648]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=1013815 ABVR74_RS02975 WP_010906131.1 565099..565746(+) (comEA) [Lactococcus lactis subsp. lactis strain CBA3648]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386