Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABWH88_RS19065 Genome accession   NZ_CP159479
Coordinates   4008122..4009837 (-) Length   571 a.a.
NCBI ID   WP_014577712.1    Uniprot ID   E4PH70
Organism   Marinobacter adhaerens strain ac5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4003122..4014837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWH88_RS19040 (ABWH88_19020) tsaA 4003591..4004334 (-) 744 WP_014577717.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  ABWH88_RS19045 - 4004334..4005427 (-) 1094 Protein_3754 hypothetical protein -
  ABWH88_RS19050 (ABWH88_19035) coaE 4005420..4006019 (-) 600 WP_014577715.1 dephospho-CoA kinase -
  ABWH88_RS19055 (ABWH88_19040) pilD 4006023..4006898 (-) 876 WP_014577714.1 A24 family peptidase Machinery gene
  ABWH88_RS19060 (ABWH88_19045) pilC 4006899..4008119 (-) 1221 WP_014577713.1 type II secretion system F family protein Machinery gene
  ABWH88_RS19065 (ABWH88_19050) pilB 4008122..4009837 (-) 1716 WP_014577712.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABWH88_RS19070 (ABWH88_19055) - 4010151..4010561 (+) 411 WP_014577711.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABWH88_RS19075 (ABWH88_19060) - 4010812..4012086 (+) 1275 WP_041645356.1 O-antigen ligase -
  ABWH88_RS19080 (ABWH88_19065) - 4012172..4012570 (+) 399 WP_041645354.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABWH88_RS19085 (ABWH88_19070) - 4012593..4013954 (-) 1362 WP_014577709.1 MBL fold metallo-hydrolase RNA specificity domain-containing protein -
  ABWH88_RS19090 (ABWH88_19075) cysQ 4014045..4014821 (+) 777 WP_014577708.1 3'(2'),5'-bisphosphate nucleotidase CysQ -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62636.47 Da        Isoelectric Point: 4.8170

>NTDB_id=1013761 ABWH88_RS19065 WP_014577712.1 4008122..4009837(-) (pilB) [Marinobacter adhaerens strain ac5]
MATDSSKVTLTGLARRFVEDGLLDEATAKDAFIQASQNRIPLITYLVQNNLADSSGLAFSAAMEFGVSVLDLSAFATEMI
PEKVVDEKLIRKHNALPIYKRGNRLFIAVSDPTNIQALDEFKFNTGLSTDAVLVDDAKLRDSIEKYLESQDTSMGDLEDA
DLEGVETEGGEQEDDNAVSASDVDDAPIVKYVNKMLLDAIRGGASDVHFEPYEKVYRVRYRTDGILKEVSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKQIYLEALE
QPQGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSVIIAQRLGRRLCNSCKQAADIPKDVLLAE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQAEGFRNLRQAALLKVIQGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1013761 ABWH88_RS19065 WP_014577712.1 4008122..4009837(-) (pilB) [Marinobacter adhaerens strain ac5]
ATGGCAACTGACAGCAGCAAAGTAACTCTCACCGGCCTGGCACGACGTTTCGTTGAGGACGGGCTTCTAGATGAAGCGAC
TGCCAAAGACGCATTCATTCAAGCCTCACAAAACCGAATACCGTTGATCACCTATCTGGTTCAGAACAACCTGGCGGATA
GCAGCGGGCTGGCATTCTCGGCTGCCATGGAATTCGGTGTTTCTGTATTGGATCTCTCGGCGTTTGCGACGGAAATGATT
CCGGAGAAGGTGGTCGACGAAAAGCTGATCCGCAAGCACAACGCTCTGCCTATTTACAAGCGCGGAAACCGGCTGTTTAT
TGCTGTTTCTGACCCGACCAATATCCAGGCGCTTGATGAGTTCAAATTCAACACCGGCCTGAGTACCGATGCGGTATTGG
TAGACGACGCAAAACTCCGTGACTCCATTGAAAAATACCTGGAATCTCAGGATACCTCCATGGGGGATCTGGAGGATGCA
GACCTTGAGGGTGTAGAGACAGAGGGCGGCGAACAGGAAGACGACAACGCTGTTTCTGCCAGTGATGTCGACGATGCACC
TATCGTGAAGTATGTGAACAAAATGTTGCTGGATGCGATTCGCGGCGGCGCATCGGATGTGCATTTCGAACCTTATGAAA
AGGTTTATCGGGTTCGCTACCGCACGGACGGTATTTTGAAAGAAGTGTCGCGGCCTTCCATCAAATTGGCGCCCAAAATT
TCTGCCCGGGTAAAAATCATGGCGCAGCTGGACATTTCCGAACGGCGTGTACCTCAGGATGGACGGATTAAAATGAAACT
GTCCAAAACCAAGGCCATCGACTTCCGGGTAAACACACTACCCACTCTATGGGGCGAAAAGATCGTATTGCGGATACTGG
ATCCGAGCCAGGCCAAGCTGGGCATTGATGTTCTTGGTTACGAGGAAGACCAGAAGCAGATTTATCTTGAGGCCCTCGAA
CAACCCCAAGGTATGATTCTGGTAACCGGGCCAACTGGCTCAGGTAAAACGGTTTCGCTCTACACCGGCCTGAACATCCT
AAACACTGCCGAAAGGAATATCTCAACTGCCGAGGACCCGGCCGAGATTAACCTTGAAGGGATCAATCAGGTAAACGTGA
ACACCCGAGTCGGGCTCGGCTTCGCCGAAGCATTGCGAGCCTTCCTCCGCCAGGACCCCGACGTAATCATGGTGGGTGAG
ATTCGGGACCTGGAGACCGCCAACATCGCGATCAAGGCCGCACAAACCGGCCACCTTGTTCTTTCCACACTGCACACCAA
CAGTGCCGCCGAAACGCTCACGCGGATGATGAACATGGGTGTTCCCTCATTCAACATCGCCACTTCGGTAAGTGTGATTA
TCGCGCAGCGATTGGGCAGGCGGTTGTGTAACAGCTGCAAGCAAGCCGCAGACATCCCCAAAGACGTGCTTTTGGCGGAG
GGGTTCACACAGGAACAAATTGATACAGGCTTCACGTTGTACCGCCCAAAGGGCTGTGATAAATGCAATGGGGGATACAA
AGGCCGCGTTGGTATTTACGAGGTGGTCAAGATTACCGACGAGCTGGCAAACATGATTATGGAAGAGGCCAGTTCCATCA
AAATTGCAAAACAGGCTCAGGCAGAGGGCTTCCGTAACTTGCGGCAGGCAGCGCTTCTGAAGGTGATTCAGGGTGTGACC
AGTCTTGAGGAAGCCAATCGCGTGACGAAGGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4PH70

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.168

100

0.573
  pilB Acinetobacter baylyi ADP1

55.42

100

0.555
  pilB Legionella pneumophila strain ERS1305867

53.217

100

0.536
  pilB Vibrio parahaemolyticus RIMD 2210633

51.619

97.373

0.503
  pilB Vibrio cholerae strain A1552

54.423

91.068

0.496
  pilB Vibrio campbellii strain DS40M4

50.177

98.774

0.496
  pilF Neisseria gonorrhoeae MS11

48.142

98.949

0.476
  pilB/pilB1 Synechocystis sp. PCC 6803

39.279

97.198

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.304

92.119

0.371

Multiple sequence alignment