Detailed information NTG1013433

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N011_RS23950
• Genome accession: NZ_CP159362
• Coordinates: 5273809..5274975 (-)
• Length: 388 a.a.
• NCBI ID: WP_024688291.1
• Uniprot ID: A0AAU8LFN2
• Organism: Pseudomonas syringae CC1417
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5268809..5279975

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N011_RS23935 (N011_23935)	-	5269967..5271382 (+)	1416	WP_024688288.1	sigma-54-dependent Fis family transcriptional regulator	-
N011_RS23940 (N011_23940)	-	5271638..5273029 (+)	1392	WP_024695330.1	GntP family permease	-
N011_RS23945 (N011_23945)	-	5273040..5273798 (+)	759	WP_024695331.1	3-hydroxybutyrate dehydrogenase	-
N011_RS23950 (N011_23950)	pilU	5273809..5274975 (-)	1167	WP_024688291.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N011_RS23955 (N011_23955)	-	5275087..5275362 (+)	276	WP_004879573.1	peptidylprolyl isomerase	-
N011_RS23960 (N011_23960)	pgm	5275418..5277064 (-)	1647	WP_024695332.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
N011_RS23965 (N011_23965)	-	5277172..5279448 (-)	2277	WP_032610620.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 42742.03 Da
• Isoelectric Point: 6.6701

>NTDB_id=1013433 N011_RS23950 WP_024688291.1 5273809..5274975(-) (pilU) [Pseudomonas syringae CC1417]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPVGTETLKPGDVAKIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLYLPPILLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNEFNELKGIMEKSGSLGMQTFDTAL
FQLAVEGAISEEEALKHADSQNNLRLRLKLHDEGSATQVTTAPAAPVGSSKPNMSEWGLVGDEPPSKG

Nucleotide

• Length: 1167 bp

>NTDB_id=1013433 N011_RS23950 WP_024688291.1 5273809..5274975(-) (pilU) [Pseudomonas syringae CC1417]
ATGGATTTCCCGGCGTTGTTGAAGATTCTGGCCAGCCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCGCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCTGTGGGTACTGAAACACTCAAGCCCGGTGACGTGGCTAAGATCGCCCAGGGCC
TCATGGATGAGGAACAGAAACTCGAATTCCTGCGCGAGCTGGAAATGAACCTGGCCGTATCACTGGCCGGCATCGGGCGT
TTCCGGATCAACATCTTCATGCAGCGCAATGAAGTGTCCATCGTCGCGCGTAACATCAAGCTGGATATTCCGCGTTTCGA
AGACCTGTATTTGCCGCCGATCCTGCTTGACGTGATCATGGAAAAGCACGGTCTGGTCCTGTTCGTGGGCGCAACCGGTT
CGGGCAAATCCACCTCGCTGGCGGCGTTGATCGATTACCGAAACCGCAATGCCAGCGGCCATATCATCACCATCGAAGAC
CCGGTCGAGTTCATCCATCGGCACAAGAAATCCATCGTCAATCAGCGTGAAGTAGGCGTCGACACCCGCAGCTTCAGGGC
TGCGCTGAAAAATACGCTGCGCCAGGCACCGGATGTGATTCTGATCGGTGAGATTCGCGACCGGGAAACCATGGAGCATG
CGCTGGCGTTCGCTGACACCGGGCATCTGGCGATCTCGACCCTGCACGCCAATAACGCCAACCAGGCACTGGACCGGATC
ATCAATTTCTTTCCCGAAGAGCGCCGCGCGCAGTTGCTGCACGACCTGGGCAACAACCTCAAGGCCTTCGTGTCCCAGCG
CCTGGTCAAAACCCCGGATGGCAAGCGTCGCGCGGCGGTGGAGGTGATGATGGGCACGCCGACGATCCGTGATTTGATCC
AGCGCAACGAGTTCAACGAGCTCAAGGGCATTATGGAAAAATCCGGCAGCCTGGGCATGCAGACGTTCGATACCGCGCTG
TTTCAACTGGCGGTGGAAGGGGCTATCAGCGAAGAGGAGGCCCTGAAGCACGCCGACTCGCAGAACAACCTTCGTCTGCG
CCTCAAACTGCACGATGAGGGCAGCGCGACGCAGGTGACCACTGCGCCTGCCGCCCCCGTCGGGTCGAGCAAACCCAACA
TGTCCGAGTGGGGCCTGGTCGGTGACGAGCCGCCGTCCAAAGGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.576	93.557	0.539
pilU	Acinetobacter baylyi ADP1	52.661	92.01	0.485
pilU	Vibrio cholerae strain A1552	51.714	90.206	0.466
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.481	99.227	0.392
pilT	Legionella pneumophila strain Lp02	42.216	86.082	0.363
pilT	Legionella pneumophila strain ERS1305867	42.216	86.082	0.363
pilT	Pseudomonas aeruginosa PAK	40.988	88.66	0.363