Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ABS648_RS28160 Genome accession   NZ_CP158373
Coordinates   6201810..6203885 (-) Length   691 a.a.
NCBI ID   WP_350447194.1    Uniprot ID   A0AAU7Y1E3
Organism   Pseudomonas solani strain T3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6196810..6208885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABS648_RS28145 (ABS648_28125) - 6198441..6200120 (-) 1680 WP_350447193.1 AAA family ATPase -
  ABS648_RS28150 (ABS648_28130) aroB 6200131..6201234 (-) 1104 WP_021221339.1 3-dehydroquinate synthase -
  ABS648_RS28155 (ABS648_28135) aroK 6201286..6201804 (-) 519 WP_081672329.1 shikimate kinase AroK -
  ABS648_RS28160 (ABS648_28140) pilQ 6201810..6203885 (-) 2076 WP_350447194.1 type IV pilus secretin PilQ Machinery gene
  ABS648_RS28165 (ABS648_28145) pilP 6203939..6204469 (-) 531 WP_043245818.1 type 4a pilus biogenesis lipoprotein PilP -
  ABS648_RS28170 (ABS648_28150) pilO 6204466..6205089 (-) 624 WP_043245819.1 type 4a pilus biogenesis protein PilO -
  ABS648_RS28175 (ABS648_28155) - 6205086..6205658 (-) 573 WP_043245820.1 PilN domain-containing protein -
  ABS648_RS28180 (ABS648_28160) pilM 6205658..6206722 (-) 1065 WP_021221345.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 73970.03 Da        Isoelectric Point: 4.8258

>NTDB_id=1011068 ABS648_RS28160 WP_350447194.1 6201810..6203885(-) (pilQ) [Pseudomonas solani strain T3]
MNSSLSRLGISLLAAMLSPVLLAADLQGLDVAALPGGKVELKLAFDEPIVAPRGYTIEQPARIALDLPGVANKLGSKNRE
LGVGNARSVTIVEAKDRTRLIVNLATLVPYSTRVEGNNLFVIVGEAAVAGQQVASSAPVAAPAAAAPVKAFAAVGKAINN
IDFQRGDQGEGNVVITLSDPTVSPDIQEQGGKIRLDFSKTQLPESLRVRLDVKDFATPVQFVSASGSSGNASITIEPTGY
YDYLAYQTDNKLTVSIKPLTQDDVEKRKNERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADIAKLFQSVTSGNES
SDERGSITVDDRTNSIIAYQTQDKLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVNWRGARVGDNNFVIGGDGG
IRTGGGGTSPNAALGTFVDMGATGATSSLGIGFVTDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGAEIPYQ
EASSSGASTTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFSNALNGVPPINKNEVNAKVLVSDGETIVIGGVFSNT
QTKATDKVPFLGDLPFLGRMFKRDVVQDKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=1011068 ABS648_RS28160 WP_350447194.1 6201810..6203885(-) (pilQ) [Pseudomonas solani strain T3]
ATGAACAGCTCGCTTTCGCGCCTCGGTATTTCTCTATTGGCCGCCATGTTGTCGCCAGTACTGCTGGCCGCTGACCTGCA
GGGCCTCGATGTAGCGGCGTTGCCTGGTGGCAAGGTCGAACTGAAACTCGCTTTTGATGAGCCCATTGTGGCGCCGCGTG
GCTATACCATCGAGCAACCGGCGCGCATCGCTCTGGATCTTCCCGGGGTGGCCAACAAGCTGGGCAGCAAGAACCGTGAG
CTCGGCGTCGGTAACGCGCGCAGCGTCACTATCGTCGAAGCTAAAGACCGTACCCGGTTGATCGTCAATCTAGCGACACT
CGTGCCTTACAGCACGCGTGTTGAGGGTAACAATCTCTTCGTCATAGTTGGTGAAGCAGCTGTTGCCGGTCAGCAAGTTG
CCAGTTCCGCTCCTGTGGCGGCGCCCGCCGCAGCTGCGCCCGTCAAGGCTTTTGCGGCGGTCGGCAAGGCAATCAACAAT
ATCGACTTCCAGCGCGGCGATCAGGGCGAGGGCAATGTTGTCATCACTCTGTCCGACCCGACTGTGAGCCCAGATATTCA
GGAGCAGGGTGGCAAGATCCGCCTCGACTTCTCCAAGACCCAGCTTCCCGAGTCTCTGCGTGTCCGTCTTGATGTGAAAG
ACTTCGCGACCCCTGTTCAATTCGTCAGTGCTTCTGGCTCCTCGGGGAACGCCAGTATCACCATCGAGCCCACTGGCTAT
TACGACTATCTGGCGTATCAGACCGACAACAAGCTCACCGTCAGCATCAAGCCGTTGACGCAGGACGATGTCGAGAAGCG
CAAGAACGAGCGCTTTGCCTACTCCGGCGAAAAGCTTTCGCTGAACTTCCAGGATATCGATGTACGTTCGGTGCTGCAGT
TGATCGCCGACTTCACCGACCTCAACCTGGTGGCCTCGGATACCGTTCAAGGCAATATCACGCTGCGCCTGCAGAATGTG
CCCTGGGATCAAGCGCTGGACCTCGTCCTGAAGACCAAGGGTCTCGACAAACGCAAGGTGGGTAACGTGCTGCTGGTCGC
TCCGGCAGACGAGATCGCTGCGCGTGAACGCCAGGAGCTCGAGTCTCAGAAGCAGATTGCCGAACTGGCACCGCTGCGTC
GCGAGCTTATCCAGGTGAACTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCCGTGACCAGTGGCAACGAATCC
TCGGATGAGCGCGGCTCGATCACTGTGGATGACCGCACCAACAGCATCATCGCCTACCAGACCCAGGACAAACTGGACGA
ACTGCGCCGTATCGTTGCGCAGCTCGATATCCCGGTCCGTCAGGTCATGATCGAGGCCCGTATCGTTGAAGCCAACGTGG
ACTACGACAAGTCGCTGGGCGTGAACTGGCGCGGGGCTCGGGTAGGCGACAACAACTTCGTGATTGGTGGTGACGGTGGT
ATCCGTACAGGCGGAGGCGGCACTTCTCCGAACGCCGCGCTTGGTACCTTCGTTGATATGGGGGCCACGGGGGCCACTTC
TTCCCTCGGCATCGGATTCGTAACCGACAACACCATCCTCGACCTCGAACTCTCCGCCATGGAGAAGACCGGTAACGGCG
AGATCGTGTCCCAGCCCAAGGTCGTTACCTCGGACAAGGAAACCGCCAAGATCCTGAAGGGCGCTGAGATTCCGTATCAG
GAAGCCAGCTCCAGCGGTGCCAGCACCACCTCCTTCAAGGAAGCGGCCCTGTCCCTGGAAGTGACCCCGCAGATCACGCC
TGACAACCGCATCATCATGGAAGTCAAGGTGACCAAGGATGCCCCGGACTTCTCCAACGCGTTGAATGGCGTACCGCCGA
TCAACAAGAACGAGGTCAACGCCAAGGTTCTGGTCAGCGATGGCGAAACCATCGTGATCGGTGGTGTGTTCTCCAACACC
CAGACCAAGGCGACCGACAAGGTTCCCTTCCTCGGTGACCTGCCGTTCCTCGGCCGCATGTTCAAGCGTGATGTAGTCCA
GGACAAGAAATCCGAGCTGCTGGTCTTCCTCACCCCGCGTATCATGAACAACCAGGCTATTGCGGTGAGTCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-121)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Secretin and TonB N terminus short domain (STN)

(302-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.762

100

0.805
  comQ Acinetobacter baylyi ADP1

43.169

99.566

0.43
  pilQ Acinetobacter baumannii strain A118

40.656

100

0.412
  pilQ Acinetobacter baumannii D1279779

40.656

100

0.412
  pilQ Legionella pneumophila strain ERS1305867

37.921

98.842

0.375
  pilQ Legionella pneumophila strain Lp02

37.921

98.842

0.375

Multiple sequence alignment