Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ABS648_RS28180 Genome accession   NZ_CP158373
Coordinates   6205658..6206722 (-) Length   354 a.a.
NCBI ID   WP_021221345.1    Uniprot ID   A0AAU7Y112
Organism   Pseudomonas solani strain T3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6200658..6211722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABS648_RS28155 (ABS648_28135) aroK 6201286..6201804 (-) 519 WP_081672329.1 shikimate kinase AroK -
  ABS648_RS28160 (ABS648_28140) pilQ 6201810..6203885 (-) 2076 WP_350447194.1 type IV pilus secretin PilQ Machinery gene
  ABS648_RS28165 (ABS648_28145) pilP 6203939..6204469 (-) 531 WP_043245818.1 type 4a pilus biogenesis lipoprotein PilP -
  ABS648_RS28170 (ABS648_28150) pilO 6204466..6205089 (-) 624 WP_043245819.1 type 4a pilus biogenesis protein PilO -
  ABS648_RS28175 (ABS648_28155) - 6205086..6205658 (-) 573 WP_043245820.1 PilN domain-containing protein -
  ABS648_RS28180 (ABS648_28160) pilM 6205658..6206722 (-) 1065 WP_021221345.1 pilus assembly protein PilM Machinery gene
  ABS648_RS28185 (ABS648_28165) - 6206907..6209327 (+) 2421 WP_162164735.1 penicillin-binding protein 1A -
  ABS648_RS28190 (ABS648_28170) - 6209442..6210710 (-) 1269 WP_271659255.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37767.98 Da        Isoelectric Point: 4.4899

>NTDB_id=1011069 ABS648_RS28180 WP_021221345.1 6205658..6206722(-) (pilM) [Pseudomonas solani strain T3]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLSKAKASSKSVAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGVSSRNSERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLAAQLGSGHDELTVAVVDIGATMTTLSVLHNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTQVANP
FAEMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1011069 ABS648_RS28180 WP_021221345.1 6205658..6206722(-) (pilM) [Pseudomonas solani strain T3]
GTGCTAGGGCTCTTCAACAAGAAAGCGAATACGCTGCTGGGGATCGACATAAGCTCGACCTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCACCGAATGCTGTGGTCGAAAAGAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGTTGTCCCGTGTTCTGAGCAAGGCGAAAGCCAGCTCCAAGTCCGTGGCTGTT
GCCGTGGCGGGCTCCGCTGTAATCACCAAGACCATCGAGATGGATGCCGGTCTTTCCGACGACGAGTTGGAAAACCAGCT
GAAGATCGAGGCGGACCAGTACATCCCTTACCCGCTCGAAGAAGTCGCCATCGACTTCGAGGTGCAGGGTGTGTCTTCGC
GCAACTCCGAGCGGGTTGATGTGCTGCTTGCGGCATGCCGCAAGGAGAACGTCGAGGTCCGTGAGGCGGCGCTGGCACTG
GCGGGTCTGACCGCCAAGGTCGTCGACGTCGAAGCCTATGCGCTGGAACGTGCCTATGGGCTGCTCGCTGCGCAACTGGG
CAGCGGCCATGACGAGCTGACCGTCGCAGTGGTGGATATCGGCGCGACCATGACGACGCTGAGCGTTCTGCACAACGGGC
GGACCATCTATAGCCGCGAGCAGCTTTTTGGCGGGCGTCAGCTCACCGAGGAGATTCAGCGCCGCTATGGCCTGTCCGTG
GAAGAAGCCGGTCTTGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGTTGCAGCCGTTCAAGGATGC
AGTGGTCCAGCAGGTGTCTCGCTCGCTGCAGTTCTTCTTCGCTGCGGGTCAGTTCAACGATGTCGACTACATCCTCCTGG
CGGGCGGTACTGCGTCCATTCCTGATCTGGATCGGCTGATCCAGCAGAAGATCGGCACTCCTACCCAGGTTGCCAACCCC
TTCGCGGAGATGGCGCTGAGCGGCAAGGTCAATGCGGGTGCATTGGCCAGTGATGCCCCGGCGCTGATGATTGCCTGCGG
ACTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

48.451

100

0.486

Multiple sequence alignment