Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   LLUC023_RS06360 Genome accession   NZ_CP158368
Coordinates   1272553..1273401 (-) Length   282 a.a.
NCBI ID   WP_011676300.1    Uniprot ID   A0A896TAG0
Organism   Lactococcus cremoris strain UC023     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1267553..1278401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLUC023_RS06345 (LLUC023_06355) xerS 1267623..1268693 (+) 1071 WP_014572599.1 tyrosine recombinase XerS -
  LLUC023_RS06350 (LLUC023_06360) trmFO 1268743..1270089 (-) 1347 WP_259750806.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  LLUC023_RS06355 (LLUC023_06365) topA 1270161..1272287 (-) 2127 WP_014572601.1 type I DNA topoisomerase -
  LLUC023_RS06360 (LLUC023_06370) dprA 1272553..1273401 (-) 849 WP_011676300.1 DNA-processing protein DprA Machinery gene
  LLUC023_RS06365 (LLUC023_06375) - 1273550..1273930 (-) 381 WP_014572603.1 RidA family protein -
  LLUC023_RS06370 (LLUC023_06380) budA 1273970..1274680 (-) 711 WP_011676302.1 acetolactate decarboxylase -
  LLUC023_RS06375 (LLUC023_06385) ilvA 1274815..1276065 (-) 1251 WP_014572605.1 threonine ammonia-lyase IlvA -
  LLUC023_RS06380 (LLUC023_06390) ilvC 1276124..1277146 (-) 1023 WP_021211415.1 ketol-acid reductoisomerase -
  LLUC023_RS06385 (LLUC023_06395) ilvN 1277192..1277668 (-) 477 WP_021037263.1 acetolactate synthase small subunit -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31955.09 Da        Isoelectric Point: 9.8385

>NTDB_id=1011007 LLUC023_RS06360 WP_011676300.1 1272553..1273401(-) (dprA) [Lactococcus cremoris strain UC023]
MITNFDLFRWKKAGMTNLGVNKLLKFFRKYDIKISLRQMGQVAQVKSIPNFIEQYKNQDVKKLRIDYKKFSSFSILDDLY
PERLREIYNPPVLIFYQGNIDLLKNPKLAFVGSRLAGQSGIKAVQKIITELNQSFTIVSGLAKGIDTASHLSAIKTKTPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLVLSEYSLGEKPLKYHFPERNRIIAGLSRGVVVVEEKLRSGSLITCERALEEG
RDIFAIPGNIADGTSDGCNHLIQQGAKLVYQAQDILEEYLYN

Nucleotide


Download         Length: 849 bp        

>NTDB_id=1011007 LLUC023_RS06360 WP_011676300.1 1272553..1273401(-) (dprA) [Lactococcus cremoris strain UC023]
ATGATTACAAATTTTGATTTATTTCGTTGGAAAAAAGCAGGAATGACCAATCTTGGTGTAAATAAGCTCTTAAAATTTTT
CCGCAAATATGATATAAAAATAAGCTTACGACAAATGGGACAAGTGGCTCAAGTCAAATCAATCCCTAACTTTATCGAAC
AATATAAAAATCAGGACGTAAAGAAACTCAGGATAGACTATAAAAAATTTTCCTCCTTTTCCATTCTTGATGACCTTTAT
CCCGAGCGTCTAAGAGAAATCTACAACCCACCCGTTTTAATTTTTTATCAGGGAAATATTGATTTATTAAAAAATCCTAA
ACTTGCCTTTGTTGGAAGCCGCCTAGCAGGTCAATCAGGGATAAAAGCTGTCCAAAAAATTATCACAGAATTGAATCAAT
CCTTTACAATAGTAAGCGGGCTTGCTAAAGGGATTGACACAGCCAGCCATCTGTCAGCAATTAAAACTAAAACACCAACA
ATTGCTGTTATTGGTACAGGATTGGATATTTTCTATCCACTAGAAAATCGTAAAATACAGGAATATCTAGCAAAAAATCA
ATTAGTTTTAAGTGAATATTCATTAGGCGAAAAACCACTTAAATACCACTTTCCAGAGCGCAATCGAATTATTGCTGGTC
TTTCACGGGGAGTTGTTGTCGTGGAAGAAAAATTAAGAAGTGGCAGTTTAATCACCTGTGAAAGGGCACTGGAAGAAGGA
AGAGATATCTTTGCCATCCCTGGAAATATTGCAGATGGAACATCTGATGGCTGTAATCATCTTATTCAACAAGGAGCAAA
ACTTGTCTATCAAGCTCAAGATATTTTAGAAGAATATTTGTACAATTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(69-270)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Lactococcus lactis subsp. cremoris KW2

97.872

100

0.979
  dprA Streptococcus mutans UA159

58.993

98.582

0.582
  dprA/cilB/dalA Streptococcus mitis SK321

55.714

99.291

0.553
  dprA/cilB/dalA Streptococcus pneumoniae R6

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus pneumoniae D39

55.357

99.291

0.55
  dprA Bacillus subtilis subsp. subtilis str. 168

39.273

97.518

0.383
  dprA Vibrio campbellii strain DS40M4

35.314

100

0.379
  dprA Legionella pneumophila strain ERS1305867

38.768

97.872

0.379
  dprA Haemophilus influenzae Rd KW20

39.259

95.745

0.376

Multiple sequence alignment