Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFV67_RS02270 Genome accession   NZ_CP158116
Coordinates   477730..479418 (-) Length   562 a.a.
NCBI ID   WP_161438931.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain IS021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 472730..484418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFV67_RS02245 (ABFV67_02245) yacG 473818..474015 (-) 198 WP_040903818.1 DNA gyrase inhibitor YacG -
  ABFV67_RS02250 (ABFV67_02250) zapD 474057..474797 (-) 741 WP_004394266.1 cell division protein ZapD -
  ABFV67_RS02255 (ABFV67_02255) coaE 474829..475437 (-) 609 WP_186463684.1 dephospho-CoA kinase -
  ABFV67_RS02260 (ABFV67_02260) pilD 475443..476309 (-) 867 WP_158138884.1 A24 family peptidase Machinery gene
  ABFV67_RS02265 (ABFV67_02265) pilC 476485..477711 (-) 1227 WP_154170154.1 type II secretion system F family protein Machinery gene
  ABFV67_RS02270 (ABFV67_02270) pilB 477730..479418 (-) 1689 WP_161438931.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFV67_RS02275 (ABFV67_02275) - 479457..479906 (-) 450 WP_154170158.1 pilin -
  ABFV67_RS02280 (ABFV67_02280) nadC 480189..481079 (-) 891 WP_186463683.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABFV67_RS02285 (ABFV67_02285) ampD 481188..481733 (+) 546 WP_154170162.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ABFV67_RS02290 (ABFV67_02290) fldB 481730..482248 (-) 519 WP_154170164.1 flavodoxin FldB -
  ABFV67_RS02295 (ABFV67_02295) xerD 482434..483288 (+) 855 WP_233268213.1 site-specific tyrosine recombinase XerD -
  ABFV67_RS02300 (ABFV67_02300) - 483304..484056 (+) 753 WP_161438935.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61979.07 Da        Isoelectric Point: 5.0074

>NTDB_id=1009814 ABFV67_RS02270 WP_161438931.1 477730..479418(-) (pilB) [Vibrio metschnikovii strain IS021]
MLTNLIAILRQAQLLSLTQEKALVEHIQASGASAPEALLDLAIFDSQELSEHISQLFALPIQNLSDYAYPELCQQLGLRE
LIMRYQALPIENNGLVLTLAMADPTHLQAEEEFRFATGLAIEVVLADYKVLQGAIRRLYGRSLAGHSAQGKEISQDELAS
LVDISDDEQQQVEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESLYRVRLRCDGILVQTQQPASHLGRRLAARIKI
LAKLDIAERRLPQDGRIKLRLNATTAIDMRVSTLPTLWGEKIVLRLLDSSAASLDIDKLGYNEMQKQLYLNALKQPQGMI
LMTGPTGSGKTVSLYTGLSLLNREQVNISTAEDPVEINLPGINQVQVQPKIGFGFAKALRSFLRQDPDIVMVGEIRDVET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPNCKVAKQPNVTEQQQFDLSADD
ILYAADPNGCSECTKGYSGRVGIYEVMAFNSQLAEAIMQRASITHIEAIAKANGMQTLQESGIEKLQQGITSIDELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=1009814 ABFV67_RS02270 WP_161438931.1 477730..479418(-) (pilB) [Vibrio metschnikovii strain IS021]
ATGCTCACCAATCTCATCGCGATTTTGCGTCAGGCGCAACTACTTAGCCTGACCCAAGAAAAAGCGCTGGTCGAACATAT
CCAAGCTTCTGGCGCCAGTGCGCCGGAAGCTTTGTTGGATTTAGCGATCTTCGATAGCCAAGAGCTGAGCGAACACATCA
GTCAGCTGTTCGCACTGCCGATCCAAAACCTCAGCGATTATGCCTACCCAGAGCTGTGCCAACAGCTCGGATTACGTGAG
TTAATCATGCGCTACCAAGCGCTACCGATTGAGAATAATGGCTTGGTACTCACCTTAGCCATGGCCGACCCGACTCATCT
GCAAGCAGAAGAAGAATTTCGCTTTGCCACCGGACTCGCGATTGAAGTGGTGTTGGCAGACTATAAAGTACTACAAGGGG
CTATTCGCCGCTTATACGGACGCTCATTAGCTGGACATTCTGCGCAAGGCAAAGAGATCAGCCAAGACGAGCTAGCCAGC
TTAGTCGATATTTCTGATGATGAGCAGCAACAAGTCGAGGACCTTAGCCAAGATGACTCTCCGGTTAGCCGATTTATCAA
CCAAATTTTGCTTGATGCGGTGCGTAAAGGTGCGTCCGATATTCACTTTGAACCTTACGAGTCCCTTTATCGAGTTCGTT
TACGCTGCGATGGTATTTTGGTGCAAACCCAACAACCCGCCAGCCATTTAGGCCGCCGGCTAGCGGCGCGAATTAAAATT
TTAGCGAAACTGGATATCGCCGAGCGGCGCTTGCCACAAGACGGGCGAATTAAACTGCGCCTCAATGCCACCACCGCGAT
TGATATGCGCGTTTCCACCTTACCCACCTTATGGGGAGAAAAAATTGTCCTCCGTTTACTCGATAGCAGCGCCGCCAGTT
TAGATATTGACAAATTAGGCTACAACGAGATGCAAAAACAGCTCTATCTCAATGCTCTCAAACAGCCACAAGGGATGATT
TTGATGACTGGCCCGACCGGCAGCGGCAAAACCGTTTCCCTTTATACTGGGCTAAGTTTATTAAATCGTGAGCAGGTGAA
TATTTCTACCGCCGAAGATCCCGTCGAAATCAATCTCCCCGGCATCAATCAGGTACAAGTGCAACCGAAAATCGGTTTTG
GCTTTGCTAAGGCGCTGCGTTCTTTTTTACGCCAAGATCCGGACATTGTTATGGTCGGTGAAATCCGCGATGTCGAGACC
GCTGAAATTGCCGTTAAAGCTGCTCAAACCGGGCATTTAGTGCTCTCCACGTTACACACTAACTCAGCAGCAGAGACCGT
GATTCGCCTGGCTAATATGGGCATTGAACCTTTTAATCTCGCTTCTTCATTAAGCTTAATCATCGCTCAGCGCTTAGCAC
GCCGTTTATGTCCGAACTGTAAAGTCGCCAAACAACCAAACGTAACGGAACAACAGCAGTTTGACTTATCAGCCGATGAC
ATTCTCTACGCGGCGGATCCGAACGGTTGCAGTGAATGCACCAAAGGCTACAGTGGCCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAATAGCCAATTAGCTGAAGCGATCATGCAGCGCGCTTCCATTACTCATATCGAAGCCATTGCTAAAGCTAATG
GTATGCAAACTTTACAAGAATCTGGAATAGAAAAACTCCAACAGGGGATCACCAGCATTGACGAGCTGCAGCGTGTTCTT
TACTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

79.359

100

0.794
  pilB Vibrio parahaemolyticus RIMD 2210633

71.174

100

0.712
  pilB Vibrio campbellii strain DS40M4

70.996

100

0.71
  pilB Legionella pneumophila strain ERS1305867

50

93.95

0.47
  pilB Acinetobacter baumannii D1279779

50.289

92.349

0.464
  pilF Neisseria gonorrhoeae MS11

44.504

100

0.447
  pilB Acinetobacter baylyi ADP1

48.643

91.815

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

37.896

100

0.404
  pilF Thermus thermophilus HB27

39.187

96.263

0.377

Multiple sequence alignment