Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABNP33_RS16785 Genome accession   NZ_CP157872
Coordinates   3551384..3553096 (+) Length   570 a.a.
NCBI ID   WP_349913666.1    Uniprot ID   -
Organism   Acinetobacter pittii strain bac73     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3546384..3558096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNP33_RS16750 (ABNP33_16745) nusA 3547272..3548756 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  ABNP33_RS16755 (ABNP33_16750) rimP 3548794..3549318 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  ABNP33_RS16775 (ABNP33_16770) secG 3549956..3550285 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  ABNP33_RS16780 (ABNP33_16775) tpiA 3550298..3551092 (-) 795 WP_046812055.1 triose-phosphate isomerase -
  ABNP33_RS16785 (ABNP33_16780) pilB 3551384..3553096 (+) 1713 WP_349913666.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABNP33_RS16790 (ABNP33_16785) pilC 3553123..3554349 (+) 1227 WP_005079343.1 type II secretion system F family protein Machinery gene
  ABNP33_RS16795 (ABNP33_16790) pilD 3554349..3555209 (+) 861 WP_002114758.1 A24 family peptidase Machinery gene
  ABNP33_RS16800 (ABNP33_16795) coaE 3555211..3555807 (+) 597 WP_002115011.1 dephospho-CoA kinase -
  ABNP33_RS16805 (ABNP33_16800) - 3555804..3556718 (-) 915 WP_005079347.1 DMT family transporter -
  ABNP33_RS16810 (ABNP33_16805) rlmB 3556754..3557503 (-) 750 WP_032052994.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABNP33_RS16815 (ABNP33_16810) - 3557606..3557932 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63624.02 Da        Isoelectric Point: 5.0932

>NTDB_id=1008708 ABNP33_RS16785 WP_349913666.1 3551384..3553096(+) (pilB) [Acinetobacter pittii strain bac73]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESSFEFNDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1008708 ABNP33_RS16785 WP_349913666.1 3551384..3553096(+) (pilB) [Acinetobacter pittii strain bac73]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACAGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGATTTTAAAACATCGGGTCTTACCTCTTATACAAAGAGGGCAGATTTTATATGT
CGCGACCAGCAATCCAAGTAATATTGAAGCGATCGATGCGATTCGCTTTAATAGTAAGCTCATGGTTGAGCCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAGCAATTTACCGAAGAAAGCAGTTTCGAGTTTAATGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCAATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAGAAATCAT
ATCGGGTTCGTTATCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCACCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTACCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
GACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACTTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCTT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTCATGTCCACACTGCATACCAATAGTGC
GCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACTTCTGTTAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATCTGACTCATCCCGATTTTCAAGTTTTCCAGCCTGTCGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTCAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTAACTTCC
TTACAAGAAGTTAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.439

100

0.954
  pilB Acinetobacter baylyi ADP1

78.246

100

0.782
  pilB Legionella pneumophila strain ERS1305867

51.675

99.474

0.514
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

51.149

91.579

0.468
  pilB Vibrio cholerae strain A1552

50.097

90.702

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

35.505

100

0.382
  pilF Thermus thermophilus HB27

36.833

98.596

0.363

Multiple sequence alignment