Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAN986_RS05765 Genome accession   NZ_CP157763
Coordinates   1281070..1282812 (-) Length   580 a.a.
NCBI ID   WP_034364744.1    Uniprot ID   A0AAX3SVK0
Organism   Delftia sp. DS1230     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1276070..1287812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAN986_RS05735 - 1276652..1277158 (+) 507 WP_016445605.1 NUDIX domain-containing protein -
  AAN986_RS05740 - 1277169..1277405 (-) 237 WP_223275109.1 DNA gyrase inhibitor YacG -
  AAN986_RS05745 zapD 1277423..1278178 (-) 756 WP_016445607.1 cell division protein ZapD -
  AAN986_RS05750 coaE 1278268..1278906 (-) 639 WP_016445608.1 dephospho-CoA kinase -
  AAN986_RS05755 pilD 1278921..1279814 (-) 894 WP_016445609.1 A24 family peptidase Machinery gene
  AAN986_RS05760 pilC 1279814..1281031 (-) 1218 WP_016445610.1 type II secretion system F family protein Machinery gene
  AAN986_RS05765 pilB 1281070..1282812 (-) 1743 WP_034364744.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAN986_RS05775 - 1283049..1283978 (-) 930 WP_043789750.1 polyprenyl synthetase family protein -
  AAN986_RS05780 rplU 1284209..1284520 (+) 312 WP_012207284.1 50S ribosomal protein L21 -
  AAN986_RS05785 rpmA 1284536..1284793 (+) 258 WP_012207283.1 50S ribosomal protein L27 -
  AAN986_RS05790 cgtA 1284943..1286058 (+) 1116 WP_016451026.1 Obg family GTPase CgtA -
  AAN986_RS05795 proB 1286142..1287290 (+) 1149 WP_016445651.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62761.81 Da        Isoelectric Point: 5.1481

>NTDB_id=1008142 AAN986_RS05765 WP_034364744.1 1281070..1282812(-) (pilB) [Delftia sp. DS1230]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAAGKAATNRTSFIAELSVAGTLSALEVAETVSTMFSAPLLDLD
AVDAMRLPQDLLDAKIISTYRVLPLSKRGNRLTVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLIAQQNKSASEA
MDSYGGSGDFEFGDLKPDEVAEEKDNSAAADVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARMGIDALGYEPEE
KARLLEAISRPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALIDAGYDDADIDGSWVTYRPVGCPACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=1008142 AAN986_RS05765 WP_034364744.1 1281070..1282812(-) (pilB) [Delftia sp. DS1230]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCATCCGCTGTCGCATCGCTTCCCGGCATCGGGCGGGCTCTGATTTCAGC
AGGCAAGGTATCGCAACAGGCAGCGGAAGCGGCTGCCGGCAAGGCCGCCACCAATCGCACCTCGTTCATTGCAGAACTGA
GCGTCGCCGGCACCCTCTCTGCCCTGGAAGTGGCGGAGACGGTTTCCACCATGTTCAGCGCGCCGCTGCTCGATCTTGAT
GCGGTCGACGCCATGCGCCTGCCGCAGGATCTGCTGGACGCCAAGATCATCAGCACCTATCGCGTCCTGCCTCTGAGCAA
GCGCGGCAACCGCCTCACGGTGGCCACCGCCGACCCGACGCAGCAGGAAGCCGCCGAGCAGATCAAGTTCACCACCCAGT
TGGGCGTGGACTGGATCATCGTCGAGGCCGACAAGCTGGAGCGGCTGATCGCGCAGCAGAACAAAAGCGCCAGCGAGGCC
ATGGACAGCTATGGCGGCAGCGGCGACTTCGAGTTCGGCGACCTCAAGCCTGATGAGGTGGCAGAGGAAAAGGACAACTC
GGCCGCAGCAGACGTCGAAGATGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACTTGCATTTCGAACCCTACGAGCACCAGTACCGGGTGCGCTTTCGCATTGACGGCGAGCTGCGTGAAATTGCATCG
CCGCCCATTGCCATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCATGGACATCTCGGAAAAGCGTGTCCC
GCAGGACGGGCGCATGAAGCTCAAGGTCGGCCCGGACCGCGTCATCGACTTTCGCGTCAGCACGCTGCCCACGCTTTTCG
GCGAGAAGATCGTGATCCGTATCCTGGACCCGAGCTCGGCCAGGATGGGCATCGACGCCCTGGGCTATGAACCCGAGGAG
AAGGCACGCCTGCTTGAAGCCATCAGCCGCCCCTACGGAATGATTCTGGTCACAGGCCCCACCGGCTCGGGCAAGACAGT
GTCGCTGTACACCTGCCTGAACCTGCTGAACAAGGCCGGCGTGAACATCGCCACGGCGGAAGATCCTTCGGAAATCAACC
TGCCCGGCGTCAACCAGGTCAATGTGAACGAAAAGGCCGGCCTCACCTTTGCCACTGCGCTCAAGGCTTTTCTGCGCCAG
GATCCCGACATCATCATGGTCGGTGAAATCCGCGATCTGGAAACGGCCGACATCTCGATCAAGGCAGCGCAAACCGGCCA
CTTGGTGCTGTCCACCCTGCACACCAATGACGCACCGACCACGTTGACGCGCATGCGCAACATGGGCATTGCGCCCTTCA
ACATCGCATCGAGCGTGATCCTGATCACCGCCCAGCGCCTGGCCAGGCGCCTGTGCCCTCAATGCAAGGAGCCGGCTGAC
ATTCCGCGGGAGGCCCTGATCGATGCAGGCTACGACGATGCGGACATTGACGGCAGCTGGGTCACCTACAGGCCCGTCGG
CTGTCCGGCATGCAACAACGGCTACAAGGGCCGCGTAGGCATCTATCAGGTGATGCCTATCAGCGAGGAAATCCAGCGCA
TCATCCTGCAGGACGGCAGTGCGCTGGAGATCGCCGCACAGGCCAAGCGCGAAGGCGTGCGGTCGCTGCGTGGCTCGGGT
TTGCACAAGGCCAGACAGGGACTGACCTCGCTCGAAGAAGTGCTGGCAGTCACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(57-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.206

97.241

0.547
  pilB Acinetobacter baylyi ADP1

55.712

98.103

0.547
  pilF Neisseria gonorrhoeae MS11

52.12

97.586

0.509
  pilB Legionella pneumophila strain ERS1305867

49.736

98.103

0.488
  pilB Vibrio cholerae strain A1552

46.679

96.034

0.448
  pilB Vibrio campbellii strain DS40M4

44.248

97.414

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

47.036

90.172

0.424
  pilF Thermus thermophilus HB27

38.889

99.31

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

83.793

0.362

Multiple sequence alignment