Detailed information NTG1008055

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ABLV49_RS17210
• Genome accession: NZ_CP157675
• Coordinates: 3567233..3568969 (-)
• Length: 578 a.a.
• NCBI ID: WP_349278348.1
• Uniprot ID: A0AAU7LPP8
• Organism: Polaromonas hydrogenivorans strain DSM 17735
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3562233..3573969

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABLV49_RS17175 (ABLV49_17175)	grxD	3562685..3563014 (+)	330	WP_011800187.1	Grx4 family monothiol glutaredoxin	-
ABLV49_RS17180 (ABLV49_17180)	-	3563054..3563314 (-)	261	WP_349278339.1	hypothetical protein	-
ABLV49_RS17185 (ABLV49_17185)	-	3563388..3563594 (-)	207	WP_349278340.1	DNA gyrase inhibitor YacG	-
ABLV49_RS17190 (ABLV49_17190)	zapD	3563653..3564408 (-)	756	WP_349278342.1	cell division protein ZapD	-
ABLV49_RS17195 (ABLV49_17195)	coaE	3564448..3565059 (-)	612	WP_349278344.1	dephospho-CoA kinase	-
ABLV49_RS17200 (ABLV49_17200)	pilD	3565064..3565915 (-)	852	WP_349281762.1	A24 family peptidase	Machinery gene
ABLV49_RS17205 (ABLV49_17205)	pilC	3565930..3567147 (-)	1218	WP_349278346.1	type II secretion system F family protein	Machinery gene
ABLV49_RS17210 (ABLV49_17210)	pilB	3567233..3568969 (-)	1737	WP_349278348.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ABLV49_RS17215 (ABLV49_17215)	-	3569196..3569510 (-)	315	WP_349278350.1	hypothetical protein	-
ABLV49_RS17220 (ABLV49_17220)	gspG	3569669..3570118 (+)	450	WP_041376506.1	type II secretion system major pseudopilin GspG	-
ABLV49_RS17225 (ABLV49_17225)	-	3570246..3570659 (+)	414	WP_349278352.1	type II secretion system protein GspH	-
ABLV49_RS17230 (ABLV49_17230)	gspI	3570659..3571036 (+)	378	WP_349278354.1	type II secretion system minor pseudopilin GspI	-
ABLV49_RS17235 (ABLV49_17235)	-	3571039..3571758 (+)	720	WP_349278356.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
ABLV49_RS17240 (ABLV49_17240)	gspK	3571863..3572786 (+)	924	WP_349281764.1	type II secretion system minor pseudopilin GspK	-

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62698.36 Da
• Isoelectric Point: 5.4632

>NTDB_id=1008055 ABLV49_RS17210 WP_349278348.1 3567233..3568969(-) (pilB) [Polaromonas hydrogenivorans strain DSM 17735]
MSATDTAIKPPVSMALPGLGRALIAAGKLGQKAAEEIFLKAQASRTSFIAELTGSGAVSAFDLAHLMSTSFAAPLVDMDA
IDNQRLPKGLLDSKICLTCRIVVLGKRNNRLIVATADPSDQGAAEKIKFSTQLGVDWVIAEYDKLSRLVDVSNASAAEAI
ENIVGSDFEFDEEMADSPGAENTEASVSEVEDAPVVKFLQKMLIDAFNMRASDLHFEPFEHSYRVRFRVDGELREISSPP
VAIKEKLAARIKVISKLDISEKRVPQDGKMKLKIGPDRVIDFRVSTLPTMFGEKIVIRILDPSSAKLGIDALGYEPAEKE
RLLEAIKRPYGMVLVTGPTGSGKTVSLYTCLNILNTAGVNIATAEDPAEITLPGVNQVSMNEKAGMTFPVALKAFLRQDP
DIIMVGEIRDVETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPHCKTPIDIP
RETLLEAGFKEDEVDGSWTPYHPVGCDKCNNGYKGRLGIYQVMPISEEIQRIVLRDGSAMEIAAQAQAEGVRSLRQSGLY
KVKLGMTSMEEILGCTNV

Nucleotide

• Length: 1737 bp

>NTDB_id=1008055 ABLV49_RS17210 WP_349278348.1 3567233..3568969(-) (pilB) [Polaromonas hydrogenivorans strain DSM 17735]
ATGTCCGCCACTGATACCGCAATAAAACCCCCAGTTTCCATGGCATTGCCTGGCTTGGGCCGGGCCTTGATCGCTGCCGG
CAAGCTGGGCCAAAAGGCGGCCGAGGAAATTTTTCTCAAGGCGCAAGCCAGTCGAACCAGTTTCATTGCCGAGCTGACAG
GCTCAGGGGCGGTGTCGGCATTCGATCTGGCGCACCTCATGTCAACGTCCTTCGCCGCGCCCCTTGTGGACATGGATGCC
ATCGACAACCAGCGTTTGCCCAAGGGCCTGCTCGACAGCAAGATATGCCTGACCTGCCGCATTGTTGTACTGGGCAAACG
CAACAACCGGCTGATCGTCGCAACCGCTGATCCATCCGATCAAGGCGCTGCGGAAAAAATCAAGTTTTCTACCCAGCTGG
GCGTGGACTGGGTCATTGCCGAATACGACAAGCTGTCCCGGCTGGTGGATGTTTCCAACGCCTCGGCTGCCGAAGCGATT
GAAAACATCGTGGGCTCGGATTTTGAATTTGATGAGGAGATGGCGGATTCGCCCGGTGCAGAAAACACTGAAGCCAGCGT
GTCTGAAGTTGAAGACGCGCCGGTTGTCAAATTCCTGCAGAAAATGCTGATTGACGCTTTCAACATGCGTGCTTCCGATC
TGCACTTTGAACCTTTCGAGCATTCCTACCGGGTCCGGTTTCGGGTCGATGGGGAGTTGCGTGAAATTTCTTCTCCGCCG
GTCGCCATCAAGGAAAAGCTGGCAGCGCGCATCAAGGTGATTTCCAAGCTGGACATTTCCGAGAAACGGGTGCCGCAGGA
CGGAAAGATGAAGCTCAAGATTGGTCCTGACCGGGTGATCGACTTCCGGGTCAGTACCCTGCCGACCATGTTTGGCGAAA
AAATCGTGATTCGTATCCTGGATCCGAGCAGCGCCAAACTGGGGATTGATGCGCTGGGCTATGAGCCGGCTGAAAAGGAG
CGCTTGCTGGAGGCCATCAAACGGCCTTACGGCATGGTTCTGGTCACCGGGCCGACGGGTTCGGGCAAAACCGTGTCGCT
CTACACCTGTCTGAATATTCTGAATACCGCCGGCGTCAACATCGCCACCGCAGAAGATCCGGCTGAAATCACTTTGCCCG
GCGTGAACCAGGTCAGCATGAACGAGAAGGCCGGAATGACTTTTCCGGTGGCCCTGAAGGCCTTTTTGCGCCAGGATCCG
GACATCATCATGGTGGGCGAAATCCGCGATGTCGAAACCGCCGACATTGCCATCAAGGCCGCGCAGACCGGCCATCTGGT
TCTGTCCACGCTGCATACCAACGATGCGCCGACGACCCTGACACGCATGCGCAATATGGGCATTGCGCCTTTCAACATCG
CGTCGAGCGTCATTCTGATTACGGCACAGAGGCTGGCGCGGCGCCTGTGCCCGCACTGCAAAACCCCGATAGACATTCCG
CGTGAAACATTGCTTGAGGCCGGCTTTAAGGAAGATGAAGTCGATGGCTCATGGACGCCTTATCATCCTGTGGGCTGCGA
CAAGTGCAACAACGGCTACAAGGGCCGCTTGGGCATTTACCAGGTCATGCCAATTTCCGAGGAAATCCAGCGCATTGTCC
TGCGTGATGGCAGCGCCATGGAAATTGCAGCCCAGGCCCAGGCTGAAGGTGTCAGGAGCCTGCGCCAGTCCGGTTTGTAC
AAAGTTAAACTGGGAATGACTTCAATGGAAGAAATTCTGGGCTGCACCAATGTTTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (55-150)

• Type II/IV secretion system protein (T2SSE) (201-468)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	52.182	99.135	0.517
pilB	Acinetobacter baumannii D1279779	52.182	99.135	0.517
pilF	Neisseria gonorrhoeae MS11	50.266	97.405	0.49
pilB	Legionella pneumophila strain ERS1305867	49.02	97.059	0.476
pilB	Vibrio parahaemolyticus RIMD 2210633	44.17	97.924	0.433
pilB	Vibrio campbellii strain DS40M4	44.207	97.059	0.429
pilB	Vibrio cholerae strain A1552	45.238	94.464	0.427
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.15	92.215	0.37
pilF	Thermus thermophilus HB27	37.9	97.232	0.369