Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ABLU27_RS01120 Genome accession   NZ_CP157500
Coordinates   140182..140829 (-) Length   215 a.a.
NCBI ID   WP_058223210.1    Uniprot ID   A0AAP3Z0X8
Organism   Lactococcus lactis strain 2B-9     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 135182..145829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLU27_RS01095 (ABLU27_01095) atpF 135251..135757 (-) 507 WP_010906128.1 F0F1 ATP synthase subunit B -
  ABLU27_RS01100 (ABLU27_01100) atpB 135772..136485 (-) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  ABLU27_RS01105 (ABLU27_01105) - 136530..136745 (-) 216 WP_004255250.1 F0F1 ATP synthase subunit C -
  ABLU27_RS01110 (ABLU27_01110) - 136930..137706 (-) 777 WP_058220836.1 alpha/beta hydrolase family protein -
  ABLU27_RS01115 (ABLU27_01115) comEC 137991..140201 (-) 2211 WP_406834732.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ABLU27_RS01120 (ABLU27_01120) comEA 140182..140829 (-) 648 WP_058223210.1 ComEA family DNA-binding protein Machinery gene
  ABLU27_RS01125 (ABLU27_01125) - 140889..142250 (-) 1362 WP_029344292.1 ABC transporter permease -
  ABLU27_RS01130 (ABLU27_01130) - 142247..143179 (-) 933 WP_406834733.1 ABC transporter ATP-binding protein -
  ABLU27_RS01135 (ABLU27_01135) - 143283..143681 (-) 399 WP_004255236.1 hypothetical protein -
  ABLU27_RS01140 (ABLU27_01140) - 143785..144348 (-) 564 WP_153241535.1 GNAT family N-acetyltransferase -
  ABLU27_RS01145 (ABLU27_01145) - 144521..145699 (-) 1179 WP_012898345.1 SLC13 family permease -

Sequence

Protein


Download         Length: 215 a.a.        Molecular weight: 23610.74 Da        Isoelectric Point: 4.8659

>NTDB_id=1007264 ABLU27_RS01120 WP_058223210.1 140182..140829(-) (comEA) [Lactococcus lactis strain 2B-9]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
VTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide


Download         Length: 648 bp        

>NTDB_id=1007264 ABLU27_RS01120 WP_058223210.1 140182..140829(-) (comEA) [Lactococcus lactis strain 2B-9]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(151-212)

SLBB domain (SLBB)

(70-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

75.688

100

0.767
  comEA/celA/cilE Streptococcus mitis SK321

44.444

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

43.119

100

0.437
  comEA/celA/cilE Streptococcus pneumoniae Rx1

41.096

100

0.419
  comEA/celA/cilE Streptococcus pneumoniae D39

41.096

100

0.419
  comEA/celA/cilE Streptococcus pneumoniae R6

41.096

100

0.419
  comEA/celA/cilE Streptococcus mitis NCTC 12261

41.284

100

0.419
  comEA Latilactobacillus sakei subsp. sakei 23K

36.564

100

0.386
  comEA Staphylococcus aureus MW2

37.559

99.07

0.372

Multiple sequence alignment