Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABLB95_RS01545 Genome accession   NZ_CP157451
Coordinates   324075..325784 (-) Length   569 a.a.
NCBI ID   WP_348908584.1    Uniprot ID   -
Organism   Acinetobacter radioresistens strain XH1688     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 319075..330784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLB95_RS01515 (ABLB95_01515) - 319207..319530 (+) 324 WP_005016998.1 pyrimidine/purine nucleoside phosphorylase -
  ABLB95_RS01520 (ABLB95_01520) rlmB 319634..320389 (+) 756 WP_017400663.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABLB95_RS01525 (ABLB95_01525) - 320414..321349 (+) 936 WP_348908585.1 DMT family transporter -
  ABLB95_RS01530 (ABLB95_01530) coaE 321346..321957 (-) 612 WP_005016991.1 dephospho-CoA kinase -
  ABLB95_RS01535 (ABLB95_01535) pilD 321959..322819 (-) 861 WP_005016989.1 A24 family peptidase Machinery gene
  ABLB95_RS01540 (ABLB95_01540) pilC 322819..324045 (-) 1227 WP_005016988.1 type II secretion system F family protein Machinery gene
  ABLB95_RS01545 (ABLB95_01545) pilB 324075..325784 (-) 1710 WP_348908584.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABLB95_RS01550 (ABLB95_01550) tpiA 326073..326867 (+) 795 WP_005023075.1 triose-phosphate isomerase -
  ABLB95_RS01555 (ABLB95_01555) secG 326878..327207 (+) 330 WP_005023077.1 preprotein translocase subunit SecG -
  ABLB95_RS01575 (ABLB95_01575) rimP 327834..328358 (+) 525 WP_026444081.1 ribosome maturation factor RimP -
  ABLB95_RS01580 (ABLB95_01580) nusA 328399..329883 (+) 1485 WP_005016980.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63653.94 Da        Isoelectric Point: 5.0245

>NTDB_id=1006966 ABLB95_RS01545 WP_348908584.1 324075..325784(-) (pilB) [Acinetobacter radioresistens strain XH1688]
MTVNFTPKFSGFIRRLVEDNHVSAAEMQNAVDTAKRNRQDIVPYLIDHFKLSPLLIAEFISIEFGEALFDLAVYDTVQIP
RDVVDEKLILKHRVLPLIKRGHTLFVATSNPTNIEALEAVRFNSKLNIEVVIVEHDKLQRIIEQNFSEESNFDFGDEELN
IDVSTSNLDEDDSQDNTPQEEEAPIVKYINKLLVDAIRIGASDLHFEPYEKYYRVRYRVDGVLRQVATPPLQLATRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFIEALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLSFSAALKSFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVAAFNIATSVNLVIAQRLARRLCNQCKVPAEIPRNSLLEMGFT
EDDLANPDFQVYQPVGCPECRDGYRGRVGIYEVMKVTPEISKIIMEDGNALEIAATAARLGFDNLRRSGLKKVMQGVTSL
QEINRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1006966 ABLB95_RS01545 WP_348908584.1 324075..325784(-) (pilB) [Acinetobacter radioresistens strain XH1688]
ATGACAGTTAATTTTACTCCAAAGTTTTCCGGTTTTATTCGCCGTCTGGTTGAGGATAACCACGTGTCTGCTGCTGAAAT
GCAGAATGCGGTCGATACTGCTAAAAGAAACCGGCAGGATATCGTGCCTTATCTGATCGATCATTTTAAGCTTTCACCCT
TACTGATTGCAGAATTTATCTCGATTGAATTTGGTGAAGCTTTATTCGATTTGGCTGTTTACGATACTGTCCAGATTCCA
CGAGATGTAGTTGATGAGAAACTGATACTCAAGCATCGGGTCTTGCCCCTCATTAAGCGTGGTCATACTCTGTTTGTTGC
TACCAGTAATCCCACCAATATTGAAGCTTTAGAAGCTGTCCGGTTTAATAGTAAACTGAATATTGAAGTTGTTATTGTCG
AGCATGACAAACTGCAGCGTATTATTGAGCAGAATTTTTCTGAAGAAAGCAATTTTGATTTTGGTGATGAAGAACTCAAT
ATTGATGTTTCAACCAGCAATTTAGATGAGGACGACAGCCAAGATAATACGCCTCAGGAAGAAGAGGCTCCAATTGTTAA
ATATATTAACAAGTTATTGGTAGATGCCATTCGTATTGGTGCATCAGACTTACATTTCGAGCCTTATGAAAAATATTACC
GGGTACGTTACCGGGTCGATGGCGTGTTAAGACAGGTTGCAACACCACCTTTACAGCTGGCTACCCGACTGGCTTCACGT
TTAAAGGTTATGTCACAGATGGATATTTCCGAAAAGCGCGTTCCCCAAGATGGCCGGATCAAATTAAAACTCTCTAAGAC
CAAAGCTATTGATTTCCGTGTGAACTCACTGCCTACACTATTTGGAGAAAAACTGGTTCTGCGTATTTTGGATCCGTCCA
GCGCCATGCTGGGTATTGATGCATTAGGCTATGAACCCGAGCAAAAAGCCCTGTTTATAGAAGCTTTAGAAAAGCCGCAA
GGTATGTTACTCATTACCGGGCCTACCGGTTCCGGTAAAACCGTATCGCTTTATACAGGCCTCAATATTTTAAACCGTGA
GGATACCAATATTTCAACTGCCGAAGACCCGGTAGAAATTAATCTTGAAGGTATTAACCAGGTCAATGTGAATAATAAAG
TCGGGTTAAGCTTCTCTGCCGCGCTAAAGTCATTCCTGCGTCAGGATCCAGATATTATTATGGTAGGTGAGATTCGTGAC
TTGGAAACAGCAGAGATTGCCATTAAAGCAGCTCAGACCGGTCACATGGTTATGTCTACCCTGCATACCAACAGCGCTCC
TGAAACATTAACACGCCTAAGAAATATGGGCGTAGCAGCATTTAATATTGCCACTTCAGTTAATCTGGTCATTGCACAGC
GTCTAGCTCGACGTTTATGCAACCAGTGCAAGGTACCAGCAGAGATTCCTAGAAATAGTCTGCTTGAGATGGGCTTTACC
GAAGATGACTTGGCCAACCCTGATTTTCAGGTTTATCAACCTGTCGGTTGCCCGGAATGTCGTGATGGTTATCGGGGTCG
TGTGGGTATCTATGAAGTAATGAAAGTTACACCGGAAATTTCAAAAATTATTATGGAAGACGGTAACGCCCTTGAAATAG
CAGCAACAGCAGCACGCCTGGGTTTTGATAACTTGCGTCGTTCTGGCCTAAAGAAAGTCATGCAAGGTGTAACCTCTTTA
CAAGAAATTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.028

99.121

0.803
  pilB Acinetobacter baylyi ADP1

78.153

98.946

0.773
  pilB Legionella pneumophila strain ERS1305867

53.493

95.606

0.511
  pilF Neisseria gonorrhoeae MS11

49.644

98.77

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

51.908

92.091

0.478
  pilB Vibrio cholerae strain A1552

50.478

91.916

0.464
  pilB Vibrio campbellii strain DS40M4

50

92.443

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

34.355

100

0.374
  pilF Thermus thermophilus HB27

36.702

99.121

0.364

Multiple sequence alignment