Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABLB96_RS04325 Genome accession   NZ_CP157428
Coordinates   888187..889899 (-) Length   570 a.a.
NCBI ID   WP_348896282.1    Uniprot ID   -
Organism   Acinetobacter sp. XH1741     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 883187..894899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLB96_RS04295 (ABLB96_04300) - 883350..883676 (+) 327 WP_348896289.1 pyrimidine/purine nucleoside phosphorylase -
  ABLB96_RS04300 (ABLB96_04305) rlmB 883779..884528 (+) 750 WP_348896288.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABLB96_RS04305 (ABLB96_04310) - 884564..885478 (+) 915 WP_348896287.1 DMT family transporter -
  ABLB96_RS04310 (ABLB96_04315) coaE 885475..886071 (-) 597 WP_348896286.1 dephospho-CoA kinase -
  ABLB96_RS04315 (ABLB96_04320) pilD 886073..886933 (-) 861 WP_348896285.1 A24 family peptidase Machinery gene
  ABLB96_RS04320 (ABLB96_04325) pilC 886933..888159 (-) 1227 WP_348896283.1 type II secretion system F family protein Machinery gene
  ABLB96_RS04325 (ABLB96_04330) pilB 888187..889899 (-) 1713 WP_348896282.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABLB96_RS04330 (ABLB96_04335) tpiA 890190..890984 (+) 795 WP_348896280.1 triose-phosphate isomerase -
  ABLB96_RS04335 (ABLB96_04340) secG 890997..891326 (+) 330 WP_348896279.1 preprotein translocase subunit SecG -
  ABLB96_RS04355 (ABLB96_04360) rimP 891963..892487 (+) 525 WP_348896278.1 ribosome maturation factor RimP -
  ABLB96_RS04360 (ABLB96_04365) nusA 892525..894009 (+) 1485 WP_263612804.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63418.85 Da        Isoelectric Point: 5.0917

>NTDB_id=1006763 ABLB96_RS04325 WP_348896282.1 888187..889899(-) (pilB) [Acinetobacter sp. XH1741]
MSALQTSPKFTGFFRRLVEEKHVSAATMQTALDAAKRAKQDIVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEYHKLEKVLGQHFAEESGFEFSDEEF
DLDVSLDGPTTQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLTHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1006763 ABLB96_RS04325 WP_348896282.1 888187..889899(-) (pilB) [Acinetobacter sp. XH1741]
ATGTCAGCATTACAAACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAACTGCATTAGATGCAGCAAAAAGAGCTAAGCAGGATATAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTGGCTGAAACAATTTCTGCTGAGTTCGCCGAACCATATTTCGATCTGGATGTTTATGACACTTCTCAGATT
CCCAAAGATTTGGTCGATCAAAAACTGGTTTTAAAGCATCGAGTTTTGCCCCTGATTCAAAGAGGGCAAATTCTATATGT
CGCGACGAGCAATCCAAGTAATATTGAAGCGATTGATGCAATCCGTTTTAACAGTAAATTGCTCGTTGAACCTGTCATTG
TTGAGTACCATAAACTTGAAAAAGTTTTAGGTCAGCATTTTGCAGAAGAAAGCGGCTTTGAATTTAGTGATGAAGAGTTT
GATCTCGACGTCAGTCTTGATGGTCCTACCACTCAAGAAGATGAAGAAGAAACGCCACAAGGCGATGAAGCACCGATCGT
AAAATATATTAATAAGTTATTGATTGATGCCATTCGTATGGGTGCTTCGGATTTACATTTTGAACCGTATGAAAAGTCTT
ATCGGGTACGTTATCGTGTCGATGGGGTATTACGTCAAATTGCTAATCCCCCTTTACAACTGGCAAACCGCTTGGCTTCG
CGTTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAACGTGTTCCACAAGATGGTCGTATCAAGCTTAAGCTATCAAA
AACCAAGGCCATTGATTTTCGTGTGAACTCACTCCCTACTTTATTTGGTGAAAAGTTAGTTCTACGTATTCTTGATCCAT
CTAGTGCAATGCTGGGTATTGATGCCTTGGGCTATGAAGAAGAACAAAAAGCTTTGTTTATGGAGGCTTTAGATAAGCCA
CAAGGCATGCTTTTAATTACTGGACCCACAGGTTCCGGTAAAACCGTATCTTTATATACGGGCTTGAATATTTTAAATAC
TGAAAGTTCCAATATTTCTACTGCCGAAGATCCGGTAGAAATTAACTTAGAAGGTATTAATCAGGTTAACGTGAACCCAA
AAGTTGGCCTTACTTTTGCTGCCGCACTTAAATCTTTTTTACGTCAAGACCCCGACATTATTATGGTGGGTGAGATTCGT
GACTTAGAAACAGCAGAAATCGCAATTAAGGCTGCGCAAACGGGTCACATGGTCATGTCAACACTGCATACGAACAGTGC
TCCAGAAACCTTGACACGCCTACGTAATATGGGGGTTCCGTCTTTCAATATTGCAACATCAGTCAACTTGGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGTAAGATACCGGCTGATATTCCTAAACAAAGTCTATTAGAAATGGGCTTT
ACCGAACAAGACTTAACACATCCAGACTTTCGGGTTTTTCAACCTGTTGGCTGTCCTGAATGTCGTGAAGGCTATAAAGG
TCGTGTGGGTATTTATGAGGTAATGAAAGTTACTCCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTCTAGAAA
TCGCGGCTGCCTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGTGTGACTTCC
TTACAGGAAATTAACCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

97.193

100

0.972
  pilB Acinetobacter baylyi ADP1

79.649

100

0.796
  pilB Legionella pneumophila strain ERS1305867

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

51.509

92.982

0.479
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.48

91.404

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365

Multiple sequence alignment