Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABHF33_RS05520 Genome accession   NZ_CP157355
Coordinates   1200984..1202690 (+) Length   568 a.a.
NCBI ID   WP_348946007.1    Uniprot ID   -
Organism   Chitinibacter sp. FCG-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1195984..1207690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHF33_RS05490 (ABHF33_05490) rplS 1196394..1196774 (-) 381 WP_348946002.1 50S ribosomal protein L19 -
  ABHF33_RS05495 (ABHF33_05495) trmD 1196813..1197574 (-) 762 WP_348946601.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ABHF33_RS05500 (ABHF33_05500) rimM 1197620..1198141 (-) 522 WP_348946003.1 ribosome maturation factor RimM -
  ABHF33_RS05505 (ABHF33_05505) rpsP 1198176..1198421 (-) 246 WP_348946004.1 30S ribosomal protein S16 -
  ABHF33_RS05510 (ABHF33_05510) ffh 1198521..1199864 (-) 1344 WP_348946005.1 signal recognition particle protein -
  ABHF33_RS05515 (ABHF33_05515) ccsA 1199946..1200752 (+) 807 WP_348946006.1 cytochrome c biogenesis protein CcsA -
  ABHF33_RS05520 (ABHF33_05520) pilB 1200984..1202690 (+) 1707 WP_348946007.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABHF33_RS05525 (ABHF33_05525) pilC 1202704..1203930 (+) 1227 WP_348946008.1 type II secretion system F family protein Machinery gene
  ABHF33_RS05530 (ABHF33_05530) pilD 1203930..1204784 (+) 855 WP_348946009.1 A24 family peptidase Machinery gene
  ABHF33_RS05535 (ABHF33_05535) coaE 1204786..1205391 (+) 606 WP_348946010.1 dephospho-CoA kinase -
  ABHF33_RS05540 (ABHF33_05540) zapD 1205427..1206185 (+) 759 WP_348946011.1 cell division protein ZapD -
  ABHF33_RS05545 (ABHF33_05545) - 1206182..1206388 (+) 207 WP_348946012.1 DNA gyrase inhibitor YacG -
  ABHF33_RS05550 (ABHF33_05550) - 1206626..1207375 (+) 750 WP_348946013.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62395.74 Da        Isoelectric Point: 5.5891

>NTDB_id=1006236 ABHF33_RS05520 WP_348946007.1 1200984..1202690(+) (pilB) [Chitinibacter sp. FCG-7]
MSTPQISGLARLLVQHGRLSETDAEALQSAASSNKTTFIEQLTQSKKLTAREVAEFSAQTFGYPLLDLDRIDSSYLPQGV
LDNNVMRAQRLVPLFKRGTKLFIGLSDITNLEAVEKVRFQTNSQVEPIIVEDNKLIVLLNQTIEATGANLKDMVIDEADL
ALEGGEQEPTAAETQMGQDIDDAPIVKYLQKVLMDAINGGASDIHFEPYEKFYRIRYRVDGVLREVAQPPLAIKEKISSR
IKVISKLDISEKRVPQDGRMKLVLSKSRAIDFRVSTLPTMHGEKIVMRILDPSSATLGIDALGYDPDQKAIILDAIQRPY
GMVLVTGPTGSGKTVSLYTCLNLLNKPDINIATAEDPCEINLPGINQVNVNDKAGLTFSAALKAFLRQDPDIIMVGEIRD
LETADIAIKAAQTGHMVFSTLHTNDAPTTLTRMLNMGIQPFNIASSVILITAQRLARRLCSCKKHVDIPNQALLDAGFNQ
EDLDGSWRPYGPTGCDICKGTGYKGRVGIYQVMPITDEINRLIMNHGNAIDIADQARREGVLSLREAGLRKVKQGLTSLE
EVMAVTNV

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1006236 ABHF33_RS05520 WP_348946007.1 1200984..1202690(+) (pilB) [Chitinibacter sp. FCG-7]
ATGAGCACCCCGCAAATTTCCGGCCTTGCCCGTTTATTGGTCCAGCACGGACGTCTAAGCGAGACGGATGCTGAAGCACT
GCAATCAGCCGCCAGTAGCAATAAAACCACTTTCATCGAACAACTGACACAAAGCAAAAAACTCACCGCCAGAGAAGTAG
CCGAGTTTTCGGCACAAACCTTTGGCTACCCTCTGCTTGACTTAGATCGCATCGACTCGAGTTACCTGCCGCAGGGCGTA
CTCGACAATAATGTCATGCGCGCACAACGCCTTGTCCCGCTTTTTAAACGTGGCACCAAGCTATTTATCGGCCTCTCTGA
CATTACCAATTTGGAAGCCGTAGAAAAAGTCCGCTTTCAAACCAATTCCCAAGTTGAACCAATTATTGTCGAAGACAATA
AGCTGATCGTTTTGCTAAACCAAACGATAGAAGCAACCGGCGCTAACCTGAAAGATATGGTCATTGACGAAGCCGACCTA
GCATTGGAGGGCGGCGAACAGGAGCCCACGGCAGCAGAAACACAAATGGGGCAAGATATTGACGATGCGCCGATTGTCAA
ATATCTGCAAAAAGTATTGATGGATGCCATCAACGGCGGCGCGTCCGATATCCACTTTGAGCCATATGAGAAATTTTACC
GGATCCGTTACCGTGTCGACGGTGTCTTGCGCGAAGTTGCTCAACCCCCACTGGCCATTAAAGAAAAAATTTCATCACGT
ATCAAAGTTATTTCCAAACTAGACATTTCGGAAAAACGCGTTCCACAAGATGGCAGGATGAAACTGGTTCTCTCCAAATC
ACGCGCGATTGATTTTCGGGTCTCAACCCTCCCCACTATGCATGGCGAGAAGATTGTAATGAGGATTTTGGACCCATCTT
CTGCCACGCTGGGGATTGATGCACTTGGCTATGATCCTGATCAAAAAGCCATCATCTTGGACGCAATCCAGCGACCTTAT
GGCATGGTGTTGGTGACAGGCCCAACGGGCTCAGGGAAGACTGTTTCGCTCTACACCTGTCTGAATTTACTCAATAAGCC
CGACATCAATATCGCAACAGCAGAAGACCCTTGCGAGATCAACTTACCTGGTATTAATCAGGTCAATGTCAACGATAAGG
CAGGGCTCACTTTTTCAGCAGCACTGAAAGCCTTTTTGCGCCAAGACCCGGACATCATCATGGTGGGCGAGATTCGCGAT
CTGGAAACTGCCGATATCGCCATTAAAGCCGCCCAAACTGGCCACATGGTTTTTTCGACGCTACATACCAACGATGCACC
AACGACACTGACCCGTATGCTTAATATGGGCATCCAGCCGTTTAATATCGCATCTTCGGTGATCTTAATCACCGCTCAGC
GCCTAGCACGGCGTCTATGCAGCTGCAAAAAGCACGTAGACATTCCAAATCAGGCCCTGCTTGATGCTGGATTTAACCAA
GAAGATCTAGATGGATCATGGCGACCTTATGGCCCAACAGGGTGTGATATTTGCAAAGGAACGGGCTACAAAGGACGGGT
AGGTATTTATCAAGTCATGCCGATTACCGACGAAATCAACCGGCTGATTATGAATCACGGCAATGCAATTGACATCGCCG
ACCAAGCTCGGCGCGAAGGTGTACTTAGCCTGCGCGAAGCCGGGTTACGTAAAGTCAAACAAGGCCTCACTTCACTCGAA
GAAGTCATGGCCGTCACCAATGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.36

100

0.555
  pilF Neisseria gonorrhoeae MS11

54.401

100

0.544
  pilB Acinetobacter baylyi ADP1

53.954

100

0.54
  pilB Legionella pneumophila strain ERS1305867

52.448

100

0.528
  pilB Vibrio cholerae strain A1552

46.726

99.472

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

44.938

99.12

0.445
  pilB Vibrio campbellii strain DS40M4

44.602

99.472

0.444
  pilF Thermus thermophilus HB27

38.313

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.021

93.134

0.382

Multiple sequence alignment