Detailed information NTG1005444

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ABKA22_RS09120
• Genome accession: NZ_CP157287
• Coordinates: 1838311..1838958 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis strain BIM B-1834
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1833311..1843958

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABKA22_RS09095 (ABKA22_09095)	atpF	1833381..1833887 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
ABKA22_RS09100 (ABKA22_09100)	atpB	1833902..1834615 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
ABKA22_RS09105 (ABKA22_09105)	-	1834660..1834875 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
ABKA22_RS09110 (ABKA22_09110)	-	1835059..1835835 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
ABKA22_RS09115 (ABKA22_09115)	comEC	1836120..1838330 (-)	2211	WP_251906613.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABKA22_RS09120 (ABKA22_09120)	comEA	1838311..1838958 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
ABKA22_RS09125 (ABKA22_09125)	-	1839018..1840379 (-)	1362	WP_021214597.1	ABC transporter permease	-
ABKA22_RS09130 (ABKA22_09130)	-	1840376..1841308 (-)	933	WP_348618757.1	ABC transporter ATP-binding protein	-
ABKA22_RS09135 (ABKA22_09135)	-	1841413..1841811 (-)	399	WP_004255236.1	hypothetical protein	-
ABKA22_RS09140 (ABKA22_09140)	-	1841915..1842478 (-)	564	WP_251906615.1	GNAT family N-acetyltransferase	-
ABKA22_RS09145 (ABKA22_09145)	-	1842651..1843829 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=1005444 ABKA22_RS09120 WP_004255240.1 1838311..1838958(-) (comEA) [Lactococcus lactis strain BIM B-1834]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=1005444 ABKA22_RS09120 WP_004255240.1 1838311..1838958(-) (comEA) [Lactococcus lactis strain BIM B-1834]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367