Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ABI122_RS20065 Genome accession   NZ_CP157205
Coordinates   3943850..3944284 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain 9.1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3938850..3949284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABI122_RS20050 (ABI122_20050) - 3940026..3940583 (+) 558 WP_000062061.1 PadR family transcriptional regulator -
  ABI122_RS20055 (ABI122_20055) metE 3941097..3943385 (+) 2289 WP_001007608.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  ABI122_RS20060 (ABI122_20060) comJ 3943424..3943813 (-) 390 WP_048655569.1 competence protein ComJ -
  ABI122_RS20065 (ABI122_20065) nucA/comI 3943850..3944284 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  ABI122_RS20070 (ABI122_20070) - 3944511..3945260 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  ABI122_RS20075 (ABI122_20075) kinB 3945299..3946573 (-) 1275 WP_000420370.1 sporulation sensor histidine kinase KinB -
  ABI122_RS20080 (ABI122_20080) - 3946884..3947084 (+) 201 WP_000929280.1 hypothetical protein -
  ABI122_RS20085 (ABI122_20085) - 3947207..3948370 (+) 1164 WP_000434578.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=1004644 ABI122_RS20065 WP_000811494.1 3943850..3944284(-) (nucA/comI) [Bacillus thuringiensis strain 9.1]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1004644 ABI122_RS20065 WP_000811494.1 3943850..3944284(-) (nucA/comI) [Bacillus thuringiensis strain 9.1]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGTTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment