Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   ABEF79_RS14115 Genome accession   NZ_CP156810
Coordinates   2837366..2837821 (-) Length   151 a.a.
NCBI ID   WP_347454119.1    Uniprot ID   -
Organism   Acinetobacter sp. ANC 7454     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2821164..2841430 2837366..2837821 within 0


Gene organization within MGE regions


Location: 2821164..2841430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF79_RS14035 (ABEF79_14035) - 2821164..2821658 (-) 495 WP_347453519.1 type IV pilin protein -
  ABEF79_RS14040 (ABEF79_14040) comE 2821652..2822098 (-) 447 WP_347453518.1 type IV pilin protein Machinery gene
  ABEF79_RS14045 (ABEF79_14045) - 2822109..2825894 (-) 3786 WP_347453517.1 pilus assembly protein PilY -
  ABEF79_RS14050 (ABEF79_14050) - 2825910..2826677 (-) 768 WP_347453516.1 pilus assembly protein PilX -
  ABEF79_RS14055 (ABEF79_14055) - 2826674..2827696 (-) 1023 WP_347453515.1 PilW family protein -
  ABEF79_RS14060 (ABEF79_14060) pilV 2827698..2828207 (-) 510 WP_180016635.1 type IV pilus modification protein PilV -
  ABEF79_RS14065 (ABEF79_14065) - 2828207..2828653 (-) 447 WP_347453514.1 GspH/FimT family pseudopilin -
  ABEF79_RS14070 (ABEF79_14070) ispH 2828818..2829768 (-) 951 WP_347453513.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ABEF79_RS14075 (ABEF79_14075) gmk 2829903..2830526 (+) 624 WP_347453512.1 guanylate kinase -
  ABEF79_RS14080 (ABEF79_14080) rpoZ 2830601..2830882 (+) 282 WP_034587719.1 DNA-directed RNA polymerase subunit omega -
  ABEF79_RS14085 (ABEF79_14085) - 2831095..2833197 (+) 2103 WP_347453511.1 HD domain-containing protein -
  ABEF79_RS14090 (ABEF79_14090) - 2833273..2833656 (+) 384 WP_347453510.1 RidA family protein -
  ABEF79_RS14095 (ABEF79_14095) - 2833845..2834039 (+) 195 WP_347453509.1 bacterioferritin-associated ferredoxin -
  ABEF79_RS14100 (ABEF79_14100) bfr 2834281..2834745 (+) 465 WP_347453508.1 bacterioferritin -
  ABEF79_RS14105 (ABEF79_14105) - 2834800..2836437 (-) 1638 WP_347453507.1 Wzy polymerase domain-containing protein -
  ABEF79_RS14110 (ABEF79_14110) tfpZ 2836525..2837262 (-) 738 WP_347460788.1 TfpX/TfpZ family type IV pilin accessory protein -
  ABEF79_RS14115 (ABEF79_14115) pilA/pilA1 2837366..2837821 (-) 456 WP_347454119.1 pilin Machinery gene
  ABEF79_RS14120 (ABEF79_14120) - 2838026..2838586 (-) 561 WP_347453505.1 TPM domain-containing protein -
  ABEF79_RS14125 (ABEF79_14125) - 2838580..2839635 (-) 1056 WP_347454118.1 TPM domain-containing protein -
  ABEF79_RS14130 (ABEF79_14130) - 2839670..2840260 (-) 591 WP_347454117.1 LemA family protein -
  ABEF79_RS14135 (ABEF79_14135) - 2840468..2841430 (+) 963 WP_075174500.1 IS30-like element IS18 family transposase -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15593.80 Da        Isoelectric Point: 5.0920

>NTDB_id=1003015 ABEF79_RS14115 WP_347454119.1 2837366..2837821(-) (pilA/pilA1) [Acinetobacter sp. ANC 7454]
MQKGFTLIELMIVVAIIGILAAIALPAYQDYTARAQASEGFKATSGLQTDIGTYLADKGALPDKADQAAGKPAESLADQA
AKLEGKYFAAGGVTLLDDGVIDIQFSAGSNANKGMKLTPNLSTAGTQIKSWTCAPSATNPIDAKRLPTSCQ

Nucleotide


Download         Length: 456 bp        

>NTDB_id=1003015 ABEF79_RS14115 WP_347454119.1 2837366..2837821(-) (pilA/pilA1) [Acinetobacter sp. ANC 7454]
ATGCAAAAGGGTTTTACCCTTATTGAATTAATGATCGTAGTTGCGATCATCGGTATTCTGGCTGCAATCGCACTTCCAGC
TTATCAAGATTATACAGCTCGTGCTCAAGCATCTGAAGGCTTTAAAGCTACTTCAGGATTGCAAACAGACATTGGTACTT
ATTTAGCAGATAAGGGAGCATTACCAGATAAAGCTGATCAAGCTGCAGGTAAACCTGCTGAATCATTAGCTGATCAGGCT
GCAAAGCTGGAAGGTAAATATTTTGCTGCTGGTGGTGTAACTTTACTTGATGATGGCGTTATCGATATTCAGTTTTCAGC
TGGTTCAAACGCAAATAAAGGTATGAAGCTCACACCTAATCTTTCAACAGCAGGAACTCAAATTAAAAGCTGGACATGTG
CTCCAAGTGCAACCAATCCTATCGATGCTAAACGCTTACCAACTAGCTGTCAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(33-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

63.816

100

0.642
  pilE Neisseria gonorrhoeae strain FA1090

38.922

100

0.43
  pilE Neisseria gonorrhoeae MS11

38.182

100

0.417
  pilA2 Legionella pneumophila str. Paris

40

99.338

0.397
  pilA2 Legionella pneumophila strain ERS1305867

40

99.338

0.397
  pilA Ralstonia pseudosolanacearum GMI1000

32.948

100

0.377
  comP Acinetobacter baylyi ADP1

36.667

99.338

0.364

Multiple sequence alignment