Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   ABEF88_RS01940 Genome accession   NZ_CP156804
Coordinates   405717..406172 (+) Length   151 a.a.
NCBI ID   WP_347454119.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7881     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 402108..430201 405717..406172 within 0


Gene organization within MGE regions


Location: 402108..430201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF88_RS01920 (ABEF88_01920) - 402108..403070 (-) 963 WP_075174500.1 IS30-like element IS18 family transposase -
  ABEF88_RS01925 (ABEF88_01925) - 403278..403868 (+) 591 WP_347454117.1 LemA family protein -
  ABEF88_RS01930 (ABEF88_01930) - 403903..404958 (+) 1056 WP_347454118.1 TPM domain-containing protein -
  ABEF88_RS01935 (ABEF88_01935) - 404952..405512 (+) 561 WP_347453505.1 TPM domain-containing protein -
  ABEF88_RS01940 (ABEF88_01940) pilA/pilA1 405717..406172 (+) 456 WP_347454119.1 pilin Machinery gene
  ABEF88_RS01945 (ABEF88_01945) tfpZ 406258..407013 (+) 756 WP_347453506.1 TfpX/TfpZ family type IV pilin accessory protein -
  ABEF88_RS01950 (ABEF88_01950) - 407101..408738 (+) 1638 WP_347453507.1 Wzy polymerase domain-containing protein -
  ABEF88_RS01955 (ABEF88_01955) bfr 408793..409257 (-) 465 WP_347453508.1 bacterioferritin -
  ABEF88_RS01960 (ABEF88_01960) - 409499..409693 (-) 195 WP_347453509.1 bacterioferritin-associated ferredoxin -
  ABEF88_RS01965 (ABEF88_01965) - 409882..410265 (-) 384 WP_347453510.1 RidA family protein -
  ABEF88_RS01970 (ABEF88_01970) - 410341..412443 (-) 2103 WP_347453511.1 HD domain-containing protein -
  ABEF88_RS01975 (ABEF88_01975) rpoZ 412656..412937 (-) 282 WP_034587719.1 DNA-directed RNA polymerase subunit omega -
  ABEF88_RS01980 (ABEF88_01980) gmk 413012..413635 (-) 624 WP_347453512.1 guanylate kinase -
  ABEF88_RS01985 (ABEF88_01985) ispH 413770..414720 (+) 951 WP_347453513.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ABEF88_RS01990 (ABEF88_01990) - 414885..415331 (+) 447 WP_347453514.1 GspH/FimT family pseudopilin -
  ABEF88_RS01995 (ABEF88_01995) pilV 415331..415840 (+) 510 WP_180016635.1 type IV pilus modification protein PilV -
  ABEF88_RS02000 (ABEF88_02000) - 415842..416864 (+) 1023 WP_347453515.1 PilW family protein -
  ABEF88_RS02005 (ABEF88_02005) - 416861..417628 (+) 768 WP_347453516.1 pilus assembly protein PilX -
  ABEF88_RS02010 (ABEF88_02010) - 417644..421429 (+) 3786 WP_347453517.1 pilus assembly protein PilY -
  ABEF88_RS02015 (ABEF88_02015) comE 421440..421886 (+) 447 WP_347453518.1 type IV pilin protein Machinery gene
  ABEF88_RS02020 (ABEF88_02020) - 421880..422374 (+) 495 WP_347453519.1 type IV pilin protein -
  ABEF88_RS02025 (ABEF88_02025) rpsP 422500..422757 (+) 258 WP_034587736.1 30S ribosomal protein S16 -
  ABEF88_RS02030 (ABEF88_02030) rimM 422787..423335 (+) 549 WP_347453520.1 ribosome maturation factor RimM -
  ABEF88_RS02035 (ABEF88_02035) trmD 423377..424126 (+) 750 WP_347453521.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ABEF88_RS02040 (ABEF88_02040) rplS 424303..424674 (+) 372 WP_004787066.1 50S ribosomal protein L19 -
  ABEF88_RS02045 (ABEF88_02045) - 424736..425719 (-) 984 WP_034587797.1 triacylglycerol lipase -
  ABEF88_RS02050 (ABEF88_02050) - 426255..427286 (-) 1032 WP_347453522.1 lipase secretion chaperone -
  ABEF88_RS02055 (ABEF88_02055) truB 427442..428347 (+) 906 WP_347453523.1 tRNA pseudouridine(55) synthase TruB -
  ABEF88_RS02060 (ABEF88_02060) - 428370..429137 (+) 768 WP_347453524.1 TSUP family transporter -
  ABEF88_RS02065 (ABEF88_02065) - 429266..429733 (+) 468 WP_347453525.1 hemerythrin domain-containing protein -
  ABEF88_RS02070 (ABEF88_02070) - 429788..430201 (-) 414 WP_347453526.1 hypothetical protein -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15593.80 Da        Isoelectric Point: 5.0920

>NTDB_id=1002963 ABEF88_RS01940 WP_347454119.1 405717..406172(+) (pilA/pilA1) [Acinetobacter thermotolerans strain ANC 7881]
MQKGFTLIELMIVVAIIGILAAIALPAYQDYTARAQASEGFKATSGLQTDIGTYLADKGALPDKADQAAGKPAESLADQA
AKLEGKYFAAGGVTLLDDGVIDIQFSAGSNANKGMKLTPNLSTAGTQIKSWTCAPSATNPIDAKRLPTSCQ

Nucleotide


Download         Length: 456 bp        

>NTDB_id=1002963 ABEF88_RS01940 WP_347454119.1 405717..406172(+) (pilA/pilA1) [Acinetobacter thermotolerans strain ANC 7881]
ATGCAAAAGGGTTTTACCCTTATTGAATTAATGATCGTAGTTGCGATCATCGGTATTCTGGCTGCAATCGCACTTCCAGC
TTATCAAGATTATACAGCTCGTGCTCAAGCATCTGAAGGCTTTAAAGCTACTTCAGGATTGCAAACAGACATTGGTACTT
ATTTAGCAGATAAGGGAGCATTACCAGATAAAGCTGATCAAGCTGCAGGTAAACCTGCTGAATCATTAGCTGATCAGGCT
GCAAAGCTGGAAGGTAAATATTTTGCTGCTGGTGGTGTAACTTTACTTGATGATGGCGTTATCGATATTCAGTTTTCAGC
TGGTTCAAACGCAAATAAAGGTATGAAGCTCACACCTAATCTTTCAACAGCAGGAACTCAAATTAAAAGCTGGACATGTG
CTCCAAGTGCAACCAATCCTATCGATGCTAAACGCTTACCAACTAGCTGTCAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(33-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

63.816

100

0.642
  pilE Neisseria gonorrhoeae strain FA1090

38.922

100

0.43
  pilE Neisseria gonorrhoeae MS11

38.182

100

0.417
  pilA2 Legionella pneumophila str. Paris

40

99.338

0.397
  pilA2 Legionella pneumophila strain ERS1305867

40

99.338

0.397
  pilA Ralstonia pseudosolanacearum GMI1000

32.948

100

0.377
  comP Acinetobacter baylyi ADP1

36.667

99.338

0.364

Multiple sequence alignment