Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABEF90_RS01530 Genome accession   NZ_CP156793
Coordinates   320351..322060 (-) Length   569 a.a.
NCBI ID   WP_347453472.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7914     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 315351..327060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF90_RS01500 (ABEF90_01500) - 315422..315745 (+) 324 WP_034587531.1 pyrimidine/purine nucleoside phosphorylase -
  ABEF90_RS01505 (ABEF90_01505) rlmB 315898..316647 (+) 750 WP_347474111.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABEF90_RS01510 (ABEF90_01510) - 316684..317604 (+) 921 WP_347453468.1 DMT family transporter -
  ABEF90_RS01515 (ABEF90_01515) coaE 317629..318228 (-) 600 WP_347453469.1 dephospho-CoA kinase -
  ABEF90_RS01520 (ABEF90_01520) pilD 318230..319090 (-) 861 WP_347453470.1 A24 family peptidase Machinery gene
  ABEF90_RS01525 (ABEF90_01525) pilC 319090..320319 (-) 1230 WP_347453471.1 type II secretion system F family protein Machinery gene
  ABEF90_RS01530 (ABEF90_01530) pilB 320351..322060 (-) 1710 WP_347453472.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABEF90_RS01535 (ABEF90_01535) tpiA 322344..323135 (+) 792 WP_347453473.1 triose-phosphate isomerase -
  ABEF90_RS01540 (ABEF90_01540) secG 323149..323478 (+) 330 WP_347453474.1 preprotein translocase subunit SecG -
  ABEF90_RS01570 (ABEF90_01570) rimP 324429..324953 (+) 525 WP_034587514.1 ribosome maturation factor RimP -
  ABEF90_RS01575 (ABEF90_01575) nusA 324989..326473 (+) 1485 WP_347453476.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63472.81 Da        Isoelectric Point: 4.8735

>NTDB_id=1002835 ABEF90_RS01530 WP_347453472.1 320351..322060(-) (pilB) [Acinetobacter thermotolerans strain ANC 7914]
MTAIQGSLRFTGFIRRLVDEGVISAPDMQSALVGAKKEKIDIVPYLIERHNISPTKIAETISTEFGEPLFDINAYDPALI
IREDFDEKIITKHRILPIFKSANILFVATSNPTNVEAIDAIRFATKLNIETIIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVDVDTSDPNKEEEETNTGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRIDGVLRQVATPPLQLATRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPKQSLLEMGFT
EEDLQNPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIQIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1002835 ABEF90_RS01530 WP_347453472.1 320351..322060(-) (pilB) [Acinetobacter thermotolerans strain ANC 7914]
ATGACAGCTATTCAGGGATCTTTACGATTTACGGGGTTTATTCGTCGACTTGTCGATGAAGGAGTTATCTCTGCACCAGA
TATGCAGAGTGCTTTGGTCGGCGCCAAAAAAGAAAAAATAGACATCGTTCCATATCTGATTGAACGCCATAATATTTCAC
CGACTAAAATCGCTGAAACCATTTCGACTGAATTTGGCGAACCGCTGTTTGACATCAATGCCTATGATCCTGCTCTGATC
ATTCGTGAAGACTTTGATGAAAAGATCATCACCAAACATCGCATCTTACCGATCTTTAAAAGTGCCAATATCCTGTTTGT
TGCCACCAGCAACCCAACCAATGTTGAAGCCATTGATGCGATCCGTTTTGCCACCAAGCTCAATATTGAAACCATCATTG
TCGAGCACAATAAACTCGAAAAGCTGATTGAACAGAATTTTACTGAAGAAAGTACCTTCGATTTTGATGATGACTTTGAT
CTAGATGTTGATGTTGATACCTCTGATCCGAATAAAGAGGAAGAAGAGACCAATACCGGTGATGAAGCACCGATTGTTAA
GTACATCAACAAGCTGCTAATTGATGCAATTCGTATGGGTGCATCAGACTTACACTTTGAACCGTATGAGAAAATATACC
GAGTCCGTTATCGTATTGACGGCGTATTGAGACAAGTGGCGACACCACCGCTGCAGCTTGCAACCCGTCTTTCCTCACGC
TTGAAAGTCATGTCGCAAATGGACATTTCAGAAAAACGGGTACCACAAGACGGTCGTATCAAGCTAAAACTTTCTAAAAA
TAAAGCAATTGATTTCCGTGTTAACTCGCTGCCGACCCTATTTGGTGAGAAAATTGTACTGCGTATCCTAGATCCATCCA
GTGCCATGCTTGGGATTGATGCATTGGGTTATGAACCTGATCAAAAAGCCCTGTTTATGGAAGCGCTGGACAAACCACAA
GGCATGCTGTTGATCACCGGTCCGACTGGTTCTGGTAAAACGGTTTCCTTATATACCGGTCTGAATATTCTAAATCGTGA
AGATACCAATATTTCTACCGCTGAAGACCCGGTGGAAATCAACTTACAAGGCATTAACCAGGTCAACGTTAACCCTAAAG
TCGGTTTAACCTTTGCTGCAGCCTTGAAATCTTTCCTGCGTCAGGATCCAGACATTGTCATGGTCGGGGAGATCCGAGAT
CTGGAAACTGCAGAAATCGCAGTGAAAGCTGCACAGACCGGTCACATGGTAATGTCAACACTACACACAAATAGTGCGCC
AGAAACACTAACCCGCTTACGCAATATGGGCGTACCGTCTTTTAACATTGCCACTTCAGTCAATCTGGTGATTGCCCAGC
GTCTGGCCCGCCGTTTATGTTCACAATGTAAAAAACCGGCCGATATTCCAAAGCAGAGCCTGCTAGAAATGGGCTTTACT
GAAGAAGATTTACAGAATCCGGAATTCCAGATTTATGAACCTGTAGGGTGCAATGAATGCCGTGAAGGTTATAAAGGCCG
TGTCGGTATTTATGAAGTCATGAAGGTGACACCGGAAATTTCACGCATTATTATGGAAGATGGCAACGCCATTCAGATTG
CCGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGATCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.825

100

0.8
  pilB Acinetobacter baylyi ADP1

76.667

100

0.768
  pilB Legionella pneumophila strain ERS1305867

52.963

94.903

0.503
  pilF Neisseria gonorrhoeae MS11

49.554

98.594

0.489
  pilB Vibrio cholerae strain A1552

48.936

99.121

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

49.013

97.891

0.48
  pilB Vibrio campbellii strain DS40M4

48.644

97.188

0.473
  pilF Thermus thermophilus HB27

37.5

98.418

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

33.605

100

0.362

Multiple sequence alignment