Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABEF82_RS01515 Genome accession   NZ_CP156787
Coordinates   320533..322242 (-) Length   569 a.a.
NCBI ID   WP_347456141.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7924     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 315533..327242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF82_RS01485 (ABEF82_01485) - 315593..315916 (+) 324 WP_034587531.1 pyrimidine/purine nucleoside phosphorylase -
  ABEF82_RS01490 (ABEF82_01490) rlmB 316070..316819 (+) 750 WP_347456136.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABEF82_RS01495 (ABEF82_01495) - 316853..317743 (+) 891 WP_347456137.1 DMT family transporter -
  ABEF82_RS01500 (ABEF82_01500) coaE 317793..318392 (-) 600 WP_347456138.1 dephospho-CoA kinase -
  ABEF82_RS01505 (ABEF82_01505) pilD 318394..319254 (-) 861 WP_347456139.1 A24 family peptidase Machinery gene
  ABEF82_RS01510 (ABEF82_01510) pilC 319269..320501 (-) 1233 WP_347456140.1 type II secretion system F family protein Machinery gene
  ABEF82_RS01515 (ABEF82_01515) pilB 320533..322242 (-) 1710 WP_347456141.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABEF82_RS01520 (ABEF82_01520) tpiA 322525..323316 (+) 792 WP_347456142.1 triose-phosphate isomerase -
  ABEF82_RS01525 (ABEF82_01525) secG 323330..323659 (+) 330 WP_034587516.1 preprotein translocase subunit SecG -
  ABEF82_RS01555 (ABEF82_01555) rimP 324610..325134 (+) 525 WP_034587514.1 ribosome maturation factor RimP -
  ABEF82_RS01560 (ABEF82_01560) nusA 325170..326654 (+) 1485 WP_347456143.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63291.53 Da        Isoelectric Point: 4.8198

>NTDB_id=1002752 ABEF82_RS01515 WP_347456141.1 320533..322242(-) (pilB) [Acinetobacter thermotolerans strain ANC 7924]
MTAIQGSLRFTGFIRRLVDEGVISAPDMQSALAGAKQEKIDIVPYLIERHNISPTKIAETISTEFGEPLFDINAYDPALI
IREDFDEKIITKHRILPIFKSANILFVATSNPTNVEAIDAIRFATKLNIETIIVEHNKLEKLIEQNFAEESTFDFDDDFD
LDVGVDASDSNTEEEDTNTGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRIDGVLRQVATPPLQLATRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKLADIPKQSLLEMGFT
EEDLQNPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIQIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1002752 ABEF82_RS01515 WP_347456141.1 320533..322242(-) (pilB) [Acinetobacter thermotolerans strain ANC 7924]
ATGACAGCTATTCAGGGATCTTTACGATTTACGGGGTTTATTCGTCGACTTGTCGATGAAGGGGTTATCTCTGCACCAGA
TATGCAGAGTGCTTTGGCCGGTGCAAAGCAAGAAAAAATCGACATCGTTCCATATCTGATTGAACGCCATAATATTTCAC
CAACTAAAATCGCTGAAACCATTTCGACTGAATTTGGCGAACCGCTGTTTGACATCAATGCCTACGATCCCGCCTTGATT
ATCCGTGAAGACTTTGATGAAAAGATCATCACCAAACATCGTATTTTGCCGATCTTTAAAAGTGCCAATATCCTGTTTGT
TGCGACCAGCAACCCGACCAATGTCGAAGCAATTGATGCGATCCGTTTTGCCACCAAGCTCAATATTGAAACCATCATTG
TCGAGCATAATAAACTCGAAAAATTGATCGAACAGAATTTTGCTGAAGAAAGTACATTCGATTTTGATGATGATTTCGAC
CTTGATGTAGGTGTGGACGCGTCTGATTCAAATACTGAAGAGGAAGACACCAATACCGGTGATGAAGCACCAATTGTTAA
GTACATTAACAAGCTGTTAATTGATGCAATTCGGATGGGTGCATCAGACTTACACTTTGAACCGTATGAAAAAATATACC
GGGTACGTTACCGTATTGATGGTGTATTAAGACAAGTAGCAACACCACCGCTTCAGCTTGCAACCCGTCTATCTTCACGT
CTCAAAGTCATGTCTCAGATGGACATTTCGGAAAAACGTGTTCCTCAGGATGGCCGTATCAAGTTAAAACTGTCCAAAAA
CAAGGCCATTGATTTCCGTGTTAACTCATTACCCACGTTGTTTGGTGAAAAAATTGTATTGCGTATCCTGGATCCGTCCA
GTGCCATGTTAGGTATTGATGCATTAGGTTATGAACCCGATCAAAAAGCCCTGTTTATGGAAGCCTTGGACAAACCACAG
GGTATGCTATTGATCACGGGTCCGACTGGTTCTGGTAAAACCGTCTCCTTATATACCGGCCTGAATATTCTGAATCGTGA
AGATACCAATATTTCTACCGCCGAAGACCCAGTGGAAATCAACTTACAGGGCATTAACCAGGTGAACGTTAACCCGAAAG
TAGGATTAACCTTCGCTGCAGCACTGAAATCATTCCTGCGTCAAGATCCGGATATCGTCATGGTCGGTGAGATCCGAGAC
TTGGAAACAGCTGAAATTGCGGTTAAAGCAGCGCAGACTGGTCACATGGTGATGTCGACACTACACACCAACAGTGCTCC
AGAAACACTGACCCGTTTACGCAATATGGGTGTACCATCATTTAACATTGCCACCTCAGTCAATCTGGTGATTGCCCAGC
GTCTGGCGCGTCGTCTGTGTTCACAATGCAAAAAACTGGCTGATATTCCAAAGCAGAGTCTGCTGGAAATGGGCTTTACC
GAAGAAGATTTACAGAACCCAGAATTCCAGATTTATGAACCTGTAGGGTGCAATGAATGCCGTGAAGGTTATAAGGGCCG
TGTCGGCATTTATGAAGTCATGAAGGTGACACCGGAAATTTCACGCATTATTATGGAAGATGGCAACGCCATTCAGATTG
CCGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGATCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.649

100

0.798
  pilB Acinetobacter baylyi ADP1

76.491

100

0.766
  pilB Legionella pneumophila strain ERS1305867

53.235

95.079

0.506
  pilB Vibrio cholerae strain A1552

49.381

99.297

0.49
  pilF Neisseria gonorrhoeae MS11

49.554

98.594

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.198

98.594

0.485
  pilB Vibrio campbellii strain DS40M4

48.282

97.188

0.469
  pilF Thermus thermophilus HB27

37.567

98.243

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

33.768

100

0.364

Multiple sequence alignment