Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ABFT82_RS19710 Genome accession   NZ_CP156749
Coordinates   4010587..4012671 (+) Length   694 a.a.
NCBI ID   WP_347507615.1    Uniprot ID   -
Organism   Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4005587..4017671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFT82_RS19690 (ABFT82_19690) pilM 4007757..4008821 (+) 1065 WP_347505747.1 pilus assembly protein PilM Machinery gene
  ABFT82_RS19695 (ABFT82_19695) pilN 4008821..4009393 (+) 573 WP_090380561.1 type 4a pilus biogenesis protein PilN -
  ABFT82_RS19700 (ABFT82_19700) pilO 4009390..4010013 (+) 624 WP_090380564.1 type 4a pilus biogenesis protein PilO -
  ABFT82_RS19705 (ABFT82_19705) pilP 4010013..4010531 (+) 519 WP_347507613.1 type 4a pilus biogenesis lipoprotein PilP -
  ABFT82_RS19710 (ABFT82_19710) pilQ 4010587..4012671 (+) 2085 WP_347507615.1 type IV pilus secretin PilQ Machinery gene
  ABFT82_RS19715 (ABFT82_19715) aroK 4012676..4013194 (+) 519 WP_090380567.1 shikimate kinase AroK -
  ABFT82_RS19720 (ABFT82_19720) aroB 4013246..4014349 (+) 1104 WP_090380570.1 3-dehydroquinate synthase -
  ABFT82_RS19725 (ABFT82_19725) - 4014360..4015940 (+) 1581 WP_090380573.1 AAA family ATPase -

Sequence

Protein


Download         Length: 694 a.a.        Molecular weight: 74604.72 Da        Isoelectric Point: 5.0479

>NTDB_id=1002529 ABFT82_RS19710 WP_347507615.1 4010587..4012671(+) (pilQ) [Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass]
MNISPSFIGLPLLAALLSPSLMAADLMALDVAALPGDRVELKLTFDEPVLAPRGYTIEQPARIALDLPGVSSKLGVKNKE
LGVGSARSVTVVEAKDRTRLVINLSSLSPYHTRAEGNSLFVVVGESSKIQAAATGSAAKPVVSAKKYSPQVRGINNIDFQ
RGEQGEGNVVITLSDASISPDIQDQGGKIRLDFAKTQLPESLRVKLDVRDFATPVHYVSTTSASDKVTVVIESSGVYDYL
AYQTDNKLTLSVKPLTQSEAEKRKADSFAYAGEKLSLNFQDVDVRSVLQLIADFTDLNLVASDTVTGNITLRLQNVPWDQ
ALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKASDMAKLFQSVTSADGTADDR
GSITVDDRTNSIIAYQTQARLDELRRIVSQLDVAVRQVMIEARIVEANVDYDKALGVNWRGARVGNSNFVVGGSGGDRVG
APGYGQNPGVQNPAPSLGNFVDLGVEGASSGIGIGFITDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGSEI
PYQEASSSGASTTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPNFDRALNGVPPIDKNEVNAKVLVSDGETIVIGGVF
SNTQAKSVDKVPFLGELPYLGRLFRRDTVKEQKSELLVFLTPRIMNNQAVAVSR

Nucleotide


Download         Length: 2085 bp        

>NTDB_id=1002529 ABFT82_RS19710 WP_347507615.1 4010587..4012671(+) (pilQ) [Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass]
ATGAACATATCCCCTTCTTTTATTGGTCTCCCTCTTCTTGCCGCGCTTCTTTCTCCGTCGTTGATGGCTGCTGATTTAAT
GGCGCTTGATGTTGCGGCTTTGCCGGGGGATCGCGTTGAGTTGAAGTTGACTTTTGATGAGCCTGTGCTTGCCCCTCGTG
GTTATACAATCGAGCAGCCTGCACGTATTGCGCTTGACTTGCCTGGTGTTTCGAGCAAGTTAGGTGTGAAAAATAAAGAG
CTCGGTGTTGGTAGCGCTCGTAGTGTCACCGTGGTTGAGGCGAAAGATCGTACTCGGCTTGTAATTAACCTATCGAGTCT
TTCTCCGTACCACACTCGCGCAGAGGGCAACAGTCTCTTTGTTGTGGTGGGCGAGAGCTCTAAAATTCAGGCTGCGGCGA
CCGGTTCTGCTGCTAAGCCCGTTGTTTCCGCGAAGAAATACTCTCCACAAGTGAGGGGAATTAACAATATAGATTTTCAG
CGTGGCGAGCAGGGCGAGGGGAATGTGGTTATAACCTTGTCTGATGCGTCCATAAGTCCTGATATACAAGATCAAGGTGG
TAAAATTCGCTTAGATTTTGCCAAGACACAGTTGCCGGAGTCTCTTCGTGTGAAGCTTGACGTGAGGGACTTTGCTACTC
CAGTACATTATGTAAGTACTACTAGTGCGTCTGATAAGGTTACTGTTGTCATTGAATCGTCTGGTGTTTATGACTACTTG
GCATACCAGACGGATAATAAGTTAACTTTAAGTGTTAAGCCGTTGACTCAGTCTGAGGCTGAGAAGCGTAAAGCCGACAG
CTTTGCTTATGCCGGTGAGAAACTTTCCCTTAACTTTCAAGATGTCGATGTTCGATCAGTTTTGCAGTTAATTGCGGATT
TCACCGACTTGAACTTGGTTGCTAGTGATACTGTTACTGGAAATATTACACTTCGGTTGCAGAATGTTCCGTGGGATCAG
GCTTTGGATCTTGTCCTAAAAACGAAAGGTTTAGATAAGCGGAAAATAGGTAACGTGCTTCTTGTAGCTCCTGCGGATGA
AATAGCTGCGCGTGAGAGGCAGGAGCTTGAATCGCAAAAGCAAATTGCTGAGCTGGCGCCTTTGCGTCGTGAGCTGATTC
AGGTGAATTATGCCAAGGCATCCGATATGGCAAAGCTTTTTCAGTCCGTAACAAGTGCAGATGGCACTGCGGATGATCGC
GGATCCATAACGGTTGATGATAGAACTAACAGCATTATTGCCTATCAAACACAAGCCCGTTTAGATGAGCTCCGTCGTAT
AGTTTCCCAGTTGGATGTCGCTGTTCGTCAGGTAATGATTGAGGCAAGAATTGTTGAGGCGAACGTTGATTACGATAAGG
CCCTTGGTGTTAATTGGCGTGGTGCGCGCGTAGGTAACAGTAATTTTGTGGTTGGCGGTAGTGGTGGTGATCGCGTTGGT
GCTCCTGGCTATGGTCAGAACCCAGGCGTTCAGAATCCTGCGCCATCTCTTGGTAATTTTGTGGACCTTGGCGTTGAAGG
TGCTAGTTCTGGGATTGGCATTGGCTTTATAACAGATAATACTATTTTGGATCTTGAACTCTCTGCCATGGAAAAAACTG
GTAACGGTGAAATCGTCTCTCAGCCTAAAGTGGTGACTTCTGACAAGGAAACGGCCAAGATTCTAAAAGGCTCAGAGATT
CCTTATCAAGAGGCTAGTTCTAGTGGCGCCAGTACTACATCTTTCAAAGAGGCAGCGCTTTCACTGGAGGTTACGCCTCA
GATTACGCCTGACAATCGTATAATTATGGAAGTAAAAGTAACAAAAGATGCGCCTAATTTTGATCGGGCGCTAAACGGAG
TCCCGCCCATAGATAAGAATGAGGTGAATGCGAAGGTTTTGGTTAGTGATGGTGAGACCATTGTTATTGGTGGTGTTTTC
TCTAATACGCAGGCAAAGTCTGTAGATAAAGTTCCTTTCTTGGGTGAGTTGCCTTATTTGGGCAGGTTGTTCAGGCGCGA
TACTGTTAAAGAGCAAAAGTCTGAACTACTTGTTTTCCTGACGCCGCGCATCATGAACAATCAAGCCGTTGCGGTGAGTC
GTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II and III secretion system protein (Secretin)

(529-685)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.853

100

0.765
  pilQ Acinetobacter baumannii D1279779

41.926

100

0.427
  pilQ Acinetobacter baumannii strain A118

41.926

100

0.427
  comQ Acinetobacter baylyi ADP1

42.94

98.991

0.425
  pilQ Legionella pneumophila strain ERS1305867

37.967

96.398

0.366
  pilQ Legionella pneumophila strain Lp02

37.967

96.398

0.366

Multiple sequence alignment