Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFG87_RS19995 Genome accession   NZ_CP156074
Coordinates   4318097..4319803 (+) Length   568 a.a.
NCBI ID   WP_136773655.1    Uniprot ID   A0A4U0PWZ3
Organism   Chitiniphilus eburneus strain YS-30     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 4309597..4327758 4318097..4319803 within 0


Gene organization within MGE regions


Location: 4309597..4327758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFG87_RS19950 - 4311109..4311951 (-) 843 WP_136773661.1 mechanosensitive ion channel family protein -
  ABFG87_RS19955 xerD 4312023..4312931 (-) 909 WP_136773660.1 site-specific tyrosine recombinase XerD -
  ABFG87_RS19960 - 4312928..4313422 (-) 495 WP_246038617.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  ABFG87_RS19965 rplS 4313508..4313888 (-) 381 WP_136773659.1 50S ribosomal protein L19 -
  ABFG87_RS19970 trmD 4313894..4314709 (-) 816 WP_373279535.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ABFG87_RS19975 rimM 4314709..4315191 (-) 483 WP_136773675.1 ribosome maturation factor RimM -
  ABFG87_RS19980 rpsP 4315258..4315506 (-) 249 WP_136773658.1 30S ribosomal protein S16 -
  ABFG87_RS19985 ffh 4315611..4316960 (-) 1350 WP_136773657.1 signal recognition particle protein -
  ABFG87_RS19990 - 4317044..4317847 (+) 804 WP_169307375.1 inner membrane protein YpjD -
  ABFG87_RS19995 pilB 4318097..4319803 (+) 1707 WP_136773655.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFG87_RS20000 pilC 4319820..4321055 (+) 1236 WP_136773654.1 type II secretion system F family protein Machinery gene
  ABFG87_RS20005 pilD 4321052..4321924 (+) 873 WP_308418438.1 A24 family peptidase Machinery gene
  ABFG87_RS20010 coaE 4321932..4322537 (+) 606 WP_136773653.1 dephospho-CoA kinase -
  ABFG87_RS20015 zapD 4322614..4323372 (+) 759 WP_136773652.1 cell division protein ZapD -
  ABFG87_RS20020 - 4323369..4323551 (+) 183 WP_211243442.1 DNA gyrase inhibitor YacG -
  ABFG87_RS20025 - 4323712..4324461 (+) 750 WP_136773650.1 GNAT family N-acetyltransferase -
  ABFG87_RS20030 - 4324655..4325335 (+) 681 WP_375591878.1 lysophospholipid acyltransferase family protein -
  ABFG87_RS20035 - 4325314..4326138 (-) 825 WP_136773648.1 symmetrical bis(5'-nucleosyl)-tetraphosphatase -
  ABFG87_RS20040 - 4326186..4327070 (+) 885 WP_136773647.1 HDOD domain-containing protein -
  ABFG87_RS20045 rplQ 4327147..4327539 (-) 393 WP_136773646.1 50S ribosomal protein L17 -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 61852.12 Da        Isoelectric Point: 5.1115

>NTDB_id=1001646 ABFG87_RS19995 WP_136773655.1 4318097..4319803(+) (pilB) [Chitiniphilus eburneus strain YS-30]
MSTTTVPISGLARLLVQHGRLVESDAEALQQSANNNKTSFIEQLISSRKISAKELAELGAQAFGYPLFDLDKIDSSYIPT
GVLDPKLMQANRLVPLFKRGSKLFVGISDITNLQGLEEVRFQSGLQVEPIVVEDGKLAALMGKLFEQSGASLKDLNVDEA
DLDIRTGEEEQPDAINADVDDAPVVKYLQKILLDAINAGASDIHFEPYEKFYRIRYRQDGVLREVAQPPLAIKEKLASRI
KVISKLDISEKRIPQDGRMKLVLSKSRAIDFRVSTLPTLHGEKICIRILDPTSATLGIDALGYDPDQKAALMAAITRPYG
MVLVTGPTGSGKTVSLYTCLNILNEPGINISTAEDPAEINLPGVNQVNVNDKAGLTFSVALKAFLRQDPDIIMVGEIRDL
ETADIAIKAAQTGHMVFSTLHTNDAPTTLTRMLNMGVAPFNIASSVILITAQRLARRLCSQCKAPADIPPEALLDAGYTE
ADLDGTWQPYRPVGCDVCKGTGYKGRLGIYQVMPISDEMNRIIMKNGTAIDIADQARAEGVRDLRHSGLLKVKQGLTSLE
EVMAVTNE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1001646 ABFG87_RS19995 WP_136773655.1 4318097..4319803(+) (pilB) [Chitiniphilus eburneus strain YS-30]
ATGTCGACCACCACCGTTCCAATTTCTGGTCTTGCACGTTTGTTGGTGCAACATGGCCGCCTGGTCGAATCGGACGCCGA
AGCACTTCAGCAGTCCGCCAACAACAACAAAACCAGCTTCATTGAACAATTGATCAGCAGCCGCAAGATTTCCGCGAAGG
AACTTGCCGAGCTGGGTGCCCAGGCATTCGGTTACCCGCTGTTCGATCTGGACAAGATCGACTCCAGCTATATCCCCACG
GGGGTGCTCGATCCCAAGCTGATGCAGGCCAATCGCCTGGTGCCGCTGTTCAAGCGGGGCAGCAAGTTGTTCGTCGGCAT
TTCGGACATCACCAATCTGCAAGGCCTGGAGGAAGTGCGCTTCCAGAGCGGCCTTCAGGTGGAACCGATCGTGGTGGAGG
ATGGCAAGCTCGCCGCCTTGATGGGCAAGCTGTTCGAGCAATCCGGCGCCAGCCTGAAAGACCTGAATGTCGATGAGGCG
GATCTCGACATTCGTACCGGCGAAGAAGAGCAACCGGATGCGATCAACGCCGATGTCGATGACGCGCCGGTCGTCAAATA
TCTGCAGAAAATCCTGCTCGACGCCATCAATGCCGGCGCCTCCGATATCCATTTCGAACCGTACGAGAAGTTCTACCGCA
TTCGCTATCGGCAGGACGGCGTCTTGCGCGAGGTCGCGCAGCCGCCATTGGCGATCAAGGAAAAGCTCGCCTCCCGTATC
AAGGTGATCTCCAAGCTGGATATTTCGGAAAAGCGGATTCCACAGGATGGCCGGATGAAGCTGGTGCTCTCCAAGAGCCG
CGCCATCGATTTCCGGGTATCCACTTTGCCCACGCTGCATGGCGAAAAAATCTGTATCCGGATTCTGGACCCGACCTCCG
CCACGCTGGGGATCGACGCACTGGGTTATGACCCGGACCAGAAGGCGGCCTTGATGGCCGCCATCACCCGTCCTTACGGC
ATGGTGCTGGTGACCGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTCTATACCTGCCTGAACATCCTGAACGAACCCGG
GATCAATATTTCGACCGCCGAAGACCCGGCCGAAATCAATTTGCCTGGCGTCAACCAGGTCAACGTGAATGACAAGGCGG
GCCTGACTTTTTCTGTCGCACTGAAGGCATTCCTGCGGCAGGATCCGGACATCATCATGGTCGGGGAAATCCGGGACCTG
GAAACGGCGGATATCGCGATCAAGGCCGCGCAAACCGGCCACATGGTCTTCTCCACGCTTCACACCAATGATGCGCCGAC
CACGCTGACGCGGATGCTGAACATGGGGGTCGCGCCGTTCAATATCGCCTCGTCGGTCATCCTGATCACGGCGCAGCGGT
TGGCGCGTCGTCTCTGTTCGCAATGCAAGGCCCCCGCGGATATCCCGCCGGAAGCCCTGCTCGATGCGGGCTACACGGAA
GCAGACCTGGATGGGACTTGGCAACCGTATCGCCCCGTGGGCTGTGACGTCTGCAAGGGAACCGGCTATAAGGGCCGACT
GGGTATCTATCAGGTCATGCCGATATCGGATGAAATGAACCGCATCATCATGAAGAACGGCACCGCCATCGATATCGCGG
ATCAGGCACGCGCGGAAGGGGTTCGCGACCTGCGCCATTCCGGCCTGCTGAAGGTGAAGCAGGGGCTCACTTCCCTCGAA
GAAGTGATGGCAGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U0PWZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.491

100

0.567
  pilF Neisseria gonorrhoeae MS11

57.219

98.768

0.565
  pilB Acinetobacter baylyi ADP1

55.497

100

0.56
  pilB Legionella pneumophila strain ERS1305867

52.098

100

0.525
  pilB Vibrio cholerae strain A1552

47.359

100

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

46.892

99.12

0.465
  pilB Vibrio campbellii strain DS40M4

46.975

98.944

0.465
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.535

98.415

0.389
  pilF Thermus thermophilus HB27

38.188

99.12

0.379

Multiple sequence alignment