Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ABFU50_RS05650 Genome accession   NZ_CP155989
Coordinates   1321344..1322600 (-) Length   418 a.a.
NCBI ID   WP_011038215.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain LMG 08006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1263210..1321637 1321344..1322600 flank -293


Gene organization within MGE regions


Location: 1263210..1322600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU50_RS05365 (ABFU50_05350) - 1263210..1265090 (+) 1881 WP_019237331.1 integrase arm-type DNA-binding domain-containing protein -
  ABFU50_RS05370 (ABFU50_05355) - 1265275..1265694 (+) 420 WP_019237332.1 hypothetical protein -
  ABFU50_RS05375 (ABFU50_05360) - 1265727..1266728 (+) 1002 WP_011038253.1 XVIPCD domain-containing protein -
  ABFU50_RS05380 (ABFU50_05365) - 1266799..1267449 (-) 651 WP_011038252.1 LysR substrate-binding domain-containing protein -
  ABFU50_RS05385 (ABFU50_05370) - 1267552..1267845 (+) 294 WP_011038251.1 helix-turn-helix domain-containing protein -
  ABFU50_RS05390 (ABFU50_05375) - 1267873..1268481 (-) 609 Protein_1042 LysR family substrate-binding domain-containing protein -
  ABFU50_RS05395 (ABFU50_05380) - 1268568..1269935 (-) 1368 WP_011035783.1 IS5-like element IS1478 family transposase -
  ABFU50_RS05400 (ABFU50_05385) - 1270051..1270269 (-) 219 Protein_1044 LysR family transcriptional regulator -
  ABFU50_RS05405 (ABFU50_05390) - 1271059..1272426 (+) 1368 WP_011035783.1 IS5-like element IS1478 family transposase -
  ABFU50_RS05410 (ABFU50_05395) - 1272534..1275062 (-) 2529 WP_254813458.1 hypothetical protein -
  ABFU50_RS05415 (ABFU50_05400) - 1275163..1276143 (-) 981 WP_011035385.1 IS5-like element IS1646 family transposase -
  ABFU50_RS05420 (ABFU50_05405) - 1276196..1277722 (-) 1527 WP_216581696.1 helix-turn-helix domain-containing protein -
  ABFU50_RS05425 (ABFU50_05410) - 1278049..1278381 (-) 333 Protein_1049 integrase core domain-containing protein -
  ABFU50_RS05430 (ABFU50_05415) - 1278412..1279514 (-) 1103 Protein_1050 IS3-like element IS1404 family transposase -
  ABFU50_RS05435 (ABFU50_05420) - 1279565..1279933 (-) 369 Protein_1051 DDE-type integrase/transposase/recombinase -
  ABFU50_RS05440 (ABFU50_05425) - 1279991..1281099 (+) 1109 WP_087942070.1 IS3 family transposase -
  ABFU50_RS05445 (ABFU50_05430) - 1281115..1281590 (-) 476 Protein_1053 IS3-like element ISXca1 family transposase -
  ABFU50_RS05450 (ABFU50_05435) - 1281915..1285178 (-) 3264 WP_407366218.1 AAA domain-containing protein -
  ABFU50_RS05455 - 1285239..1285459 (+) 221 Protein_1055 transposase -
  ABFU50_RS05460 (ABFU50_05445) - 1285639..1285959 (-) 321 WP_053051518.1 hypothetical protein -
  ABFU50_RS05465 (ABFU50_05450) - 1286368..1287906 (+) 1539 WP_011038243.1 MASE1 domain-containing protein -
  ABFU50_RS05470 (ABFU50_05455) - 1288929..1289576 (+) 648 WP_016848927.1 hypothetical protein -
  ABFU50_RS05475 (ABFU50_05460) - 1289608..1290747 (+) 1140 WP_011038241.1 type IV secretion system protein -
  ABFU50_RS05480 (ABFU50_05465) - 1290825..1291364 (+) 540 WP_225443876.1 DUF4189 domain-containing protein -
  ABFU50_RS05485 (ABFU50_05470) - 1291762..1292292 (+) 531 WP_254813456.1 hypothetical protein -
  ABFU50_RS05490 (ABFU50_05475) - 1292289..1292948 (+) 660 WP_011038238.1 hypothetical protein -
  ABFU50_RS05495 (ABFU50_05480) - 1292945..1293202 (+) 258 Protein_1063 hypothetical protein -
  ABFU50_RS05500 (ABFU50_05485) - 1293266..1293397 (+) 132 Protein_1064 transposase -
  ABFU50_RS05505 (ABFU50_05490) - 1293517..1294884 (+) 1368 WP_011035783.1 IS5-like element IS1478 family transposase -
  ABFU50_RS05510 (ABFU50_05495) - 1294874..1295613 (+) 740 Protein_1066 IS3 family transposase -
  ABFU50_RS05515 (ABFU50_05500) - 1295630..1296064 (-) 435 Protein_1067 integrase core domain-containing protein -
  ABFU50_RS05520 (ABFU50_05505) - 1296184..1297551 (+) 1368 WP_011035783.1 IS5-like element IS1478 family transposase -
  ABFU50_RS05525 (ABFU50_05510) - 1297574..1298250 (-) 677 Protein_1069 transposase -
  ABFU50_RS05530 (ABFU50_05515) - 1298325..1298624 (+) 300 Protein_1070 transposase -
  ABFU50_RS05535 (ABFU50_05520) mobH 1298649..1299647 (-) 999 Protein_1071 MobH family relaxase -
  ABFU50_RS05540 (ABFU50_05525) - 1300098..1301054 (+) 957 WP_228442279.1 GGDEF domain-containing protein -
  ABFU50_RS05545 (ABFU50_05530) - 1301604..1302530 (-) 927 WP_011038232.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
  ABFU50_RS05550 (ABFU50_05535) - 1302523..1303281 (-) 759 WP_011038231.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein -
  ABFU50_RS05555 (ABFU50_05540) - 1303506..1303865 (+) 360 WP_029628872.1 DUF3742 family protein -
  ABFU50_RS05560 (ABFU50_05545) - 1303877..1305400 (-) 1524 WP_029628873.1 conjugal transfer protein TraG N-terminal domain-containing protein -
  ABFU50_RS05565 (ABFU50_05550) - 1305416..1305763 (-) 348 WP_011269554.1 hypothetical protein -
  ABFU50_RS05570 (ABFU50_05555) - 1305760..1307097 (-) 1338 WP_309545238.1 integrating conjugative element protein -
  ABFU50_RS05575 (ABFU50_05560) - 1307171..1308109 (-) 939 WP_011038228.1 TIGR03756 family integrating conjugative element protein -
  ABFU50_RS05585 (ABFU50_05570) - 1309549..1309890 (+) 342 WP_228422811.1 hypothetical protein -
  ABFU50_RS05590 (ABFU50_05575) - 1310008..1310163 (+) 156 WP_011038226.1 hypothetical protein -
  ABFU50_RS05595 (ABFU50_05580) - 1310340..1310735 (+) 396 WP_011038225.1 hypothetical protein -
  ABFU50_RS05600 (ABFU50_05585) - 1310826..1311218 (+) 393 WP_011038224.1 H-NS family nucleoid-associated regulatory protein -
  ABFU50_RS05605 (ABFU50_05590) glgX 1311757..1313886 (-) 2130 WP_011038223.1 glycogen debranching protein GlgX -
  ABFU50_RS05610 (ABFU50_05595) rimK 1314379..1315254 (+) 876 WP_011038222.1 30S ribosomal protein S6--L-glutamate ligase -
  ABFU50_RS05615 (ABFU50_05600) - 1315904..1316581 (+) 678 WP_003490678.1 response regulator transcription factor -
  ABFU50_RS05620 (ABFU50_05605) - 1316574..1317908 (+) 1335 WP_019237347.1 HAMP domain-containing sensor histidine kinase -
  ABFU50_RS05625 (ABFU50_05610) - 1318051..1318542 (-) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  ABFU50_RS05630 (ABFU50_05615) - 1318539..1318829 (-) 291 WP_011038219.1 DUF1778 domain-containing protein -
  ABFU50_RS05635 (ABFU50_05620) - 1318904..1319305 (-) 402 WP_019237348.1 SymE family type I addiction module toxin -
  ABFU50_RS05640 (ABFU50_05625) coaE 1319837..1320460 (-) 624 WP_011038217.1 dephospho-CoA kinase -
  ABFU50_RS05645 (ABFU50_05630) - 1320474..1321337 (-) 864 WP_011038216.1 A24 family peptidase -
  ABFU50_RS05650 (ABFU50_05635) pilC 1321344..1322600 (-) 1257 WP_011038215.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45907.37 Da        Isoelectric Point: 10.3359

>NTDB_id=1000808 ABFU50_RS05650 WP_011038215.1 1321344..1322600(-) (pilC) [Xanthomonas campestris pv. campestris strain LMG 08006]
MSIARSSMKKQPIDRNVNEMVPFVWEGTDKRGIKMKGEQPARNANMLRAELRRQGITPLVVKTKPKPLFGAAGKKISSKD
IAFFSRQMATMMKSGVPIVGSLEIIGEGHKNPRMKKMVGQIRTDIEGGSSLHEAISRHPVQFDDLYRNLVRAGEGAGVLE
TVLDTVANYKENIEALKGKIKKALFYPAMVMAVAIIVSGILLVFVVPQFEDVFKGFGAELPAFTQMIVAASRFMVSYWWL
MLLGSIAAIAGFIFAYKRSPRMRHGMDRLVLKVPVIGQIMHNSSIARFARTTAVTFKAGVPLVEALSIVAGATGNKVYEE
AVLRMRDDVSVGYPVNMAMKQVNLFPHMVVQMTSIGEEAGALDAMLFKVAEYFEQEVNNAVDALSSLLEPLIMVFIGTIV
GGMVIGMYLPIFKLGSVV

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=1000808 ABFU50_RS05650 WP_011038215.1 1321344..1322600(-) (pilC) [Xanthomonas campestris pv. campestris strain LMG 08006]
ATGTCAATCGCTCGTAGCAGCATGAAGAAGCAGCCGATAGACCGGAACGTAAACGAGATGGTCCCATTTGTCTGGGAGGG
TACGGACAAGCGCGGCATCAAGATGAAGGGGGAACAACCTGCTCGCAACGCGAACATGCTCCGCGCTGAGCTTCGGCGGC
AGGGCATCACACCTTTAGTTGTAAAGACGAAGCCTAAGCCGCTTTTCGGCGCCGCTGGCAAGAAAATTTCTTCAAAAGAT
ATCGCATTTTTCAGTCGCCAGATGGCAACAATGATGAAGTCGGGCGTACCTATTGTTGGATCGCTTGAAATCATCGGAGA
GGGGCACAAAAATCCCCGCATGAAAAAAATGGTTGGTCAAATTAGGACCGACATTGAAGGAGGGTCTTCACTCCATGAAG
CAATTTCTAGGCATCCAGTCCAATTCGACGACCTCTATCGAAATTTGGTACGAGCAGGCGAAGGTGCCGGCGTTCTAGAA
ACCGTTTTAGACACAGTTGCAAATTACAAAGAAAACATTGAAGCACTGAAGGGAAAAATAAAGAAAGCTTTATTTTATCC
TGCAATGGTAATGGCCGTTGCAATTATTGTGAGCGGAATATTACTTGTATTTGTTGTCCCGCAATTTGAAGACGTATTCA
AAGGATTCGGAGCAGAACTACCAGCTTTCACTCAGATGATCGTCGCGGCGTCGCGTTTCATGGTGTCTTATTGGTGGCTT
ATGCTACTTGGTAGCATCGCAGCAATTGCAGGATTCATTTTTGCTTACAAGCGCTCGCCTCGCATGCGACATGGAATGGA
TAGATTGGTCTTGAAGGTCCCAGTCATTGGCCAGATCATGCATAATAGCTCTATCGCCCGATTTGCGCGAACCACTGCCG
TTACGTTCAAAGCTGGCGTTCCTTTAGTTGAAGCCTTGAGCATTGTGGCTGGCGCCACCGGTAATAAAGTTTATGAGGAA
GCTGTGCTTCGTATGCGCGATGATGTCTCGGTAGGCTACCCTGTCAACATGGCGATGAAGCAGGTCAATCTTTTCCCACA
CATGGTGGTTCAGATGACCTCGATCGGCGAAGAAGCTGGCGCCCTGGATGCCATGTTGTTCAAAGTGGCTGAATATTTCG
AGCAAGAAGTTAATAACGCCGTGGATGCATTGAGCAGCTTACTGGAACCATTGATCATGGTTTTTATTGGCACAATTGTG
GGCGGCATGGTCATTGGCATGTATCTTCCCATCTTCAAACTTGGCTCAGTGGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(84-207)

Type II secretion system (T2SS), protein F (T2SSF)

(288-410)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.788

94.737

0.51
  pilC Acinetobacter baylyi ADP1

53.401

94.976

0.507
  pilC Acinetobacter baumannii D1279779

51.741

96.172

0.498
  pilC Legionella pneumophila strain ERS1305867

50.493

97.129

0.49
  pilG Neisseria gonorrhoeae MS11

44.836

94.976

0.426
  pilG Neisseria meningitidis 44/76-A

44.332

94.976

0.421
  pilC Vibrio cholerae strain A1552

42.172

94.737

0.4
  pilC Vibrio campbellii strain DS40M4

39.141

94.737

0.371

Multiple sequence alignment