Detailed information of TA system TA086478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 2779071..2779885
• Replicon: chromosome
• Accession: NZ_CP023960
• Organism: Escherichia coli strain FDAARGOS_448

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: CRN68_RS15555
• Protein ID: WP_001054376.1
• Coordinates: 2779071..2779328 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: CRN68_RS15560
• Protein ID: WP_001309181.1
• Coordinates: 2779340..2779885 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN68_RS15520	2774359..2775465	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
CRN68_RS15525	2775530..2776510	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
CRN68_RS15535	2777093..2778333	-	1241	Protein_2618	helicase YjhR	-
CRN68_RS15545	2778449..2778694	+	246	Protein_2619	GNAT family N-acetyltransferase	-
CRN68_RS15555	2779071..2779328	+	258	WP_001054376.1	hypothetical protein	Toxin
CRN68_RS15560	2779340..2779885	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
CRN68_RS15565	2779941..2780687	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
CRN68_RS15570	2780856..2781074	+	219	Protein_2623	hypothetical protein	-
CRN68_RS29490	2781112..2781228	+	117	Protein_2624	VOC family protein	-
CRN68_RS15580	2781473..2782594	+	1122	WP_000010829.1	M42 family metallopeptidase	-
CRN68_RS15585	2782591..2782869	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
CRN68_RS15590	2782881..2784194	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020089 4419085..4430625 0.007 View NC_000913 Escherichia coli str. K-12 substr. MG1655 Genomic island TA006279 4533239..4545029 0.002 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021460 4537919..4549709 0.002 View NC_012759 Escherichia coli BW2952 Genomic island TA023876 4469997..4481537 0.007 View NC_017638 Escherichia coli DH1 Genomic island TA027426 4513009..4524807 0.003 View NZ_CP009273 Escherichia coli BW25113 Genomic island TA048705 4523056..4534846 0.002 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Genomic island TA068445 4432698..4444488 0.002 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285034 4419085..4430625 0.007	inside	Genomic island	-	fimI / fimA / fimE / fimB	2768858..2779885	11027

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T86478 WP_001054376.1 NZ_CP023960:2779071-2779328 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T86478 NZ_CP023960:2779071-2779328 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT86478 WP_001309181.1 NZ_CP023960:2779340-2779885 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT86478 NZ_CP023960:2779340-2779885 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8