Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) HipBST/-
Location 3948463..3950089 Replicon chromosome
Accession NC_011601
Organism Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)


Gene name HipT Uniprot ID B7UL96
Locus tag E2348C_RS20315 Protein ID WP_001262465.1
Coordinates 3948463..3949470 (-) Length 336 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID B7UL97
Locus tag E2348C_RS20320 Protein ID WP_001346664.1
Coordinates 3949470..3949781 (-) Length 104 a.a.

Regulator (Protein)


Gene name HipB Uniprot ID B7UL98
Locus tag E2348C_RS20325 Protein ID WP_000563102.1
Coordinates 3949766..3950089 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2348C_RS20285 (E2348_C_3780) 3943633..3945312 + 1680 WP_000191555.1 cellulose biosynthesis protein BcsG -
E2348C_RS20290 (E2348_C_3781) 3945427..3945885 - 459 WP_000526113.1 IS200/IS605-like element IS200C family transposase -
E2348C_RS27740 (E2348C_3782) 3946109..3946216 - 108 WP_001295224.1 type I toxin-antitoxin system toxin Ldr family protein -
E2348C_RS28545 (E2348C_3783) 3946592..3946699 - 108 WP_000170747.1 type I toxin-antitoxin system toxin Ldr family protein -
E2348C_RS20310 (E2348_C_3784) 3947175..3948446 + 1272 WP_001339917.1 amino acid permease -
E2348C_RS20315 (E2348_C_3785) 3948463..3949470 - 1008 WP_001262465.1 HipA domain-containing protein Toxin
E2348C_RS20320 (E2348_C_3786) 3949470..3949781 - 312 WP_001346664.1 HipA N-terminal domain-containing protein Antitoxin
E2348C_RS20325 (E2348_C_3787) 3949766..3950089 - 324 WP_000563102.1 helix-turn-helix transcriptional regulator Regulator
E2348C_RS20330 (E2348_C_3788) 3950314..3951327 - 1014 WP_000103582.1 dipeptide ABC transporter ATP-binding subunit DppF -
E2348C_RS20335 (E2348_C_3789) 3951324..3952307 - 984 WP_001196477.1 dipeptide ABC transporter ATP-binding protein -
E2348C_RS20340 (E2348_C_3790) 3952318..3953220 - 903 WP_000084677.1 dipeptide ABC transporter permease DppC -
E2348C_RS20345 (E2348_C_3791) 3953230..3954249 - 1020 WP_000938855.1 dipeptide ABC transporter permease DppB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3945427..3945885 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(56-302)

Antitoxin

HipA, N-terminal subdomain 1

(6-99)


Sequences

Toxin        


Download         Length: 336 a.a.        Molecular weight: 38329.55 Da        Isoelectric Point: 5.4135

>T10121 WP_001262465.1 NC_011601:c3949470-3948463 [Escherichia coli O127:H6 str. E2348/69]
MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLNPRLGFTRTQFVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQ
GNFILKPSPADFPGLAENEHATMTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDNKGLPVHQEQLDGAMQITD
KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGLVYSDGLTPALAPVYDFVSVAPY
PEYFYSNYLALPLLTREEGGRELAPGFHSDYGEYIGQDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDH
IQAVLQCYRHRLGLL

Download         Length: 1008 bp

>T10121 NC_011601:c3949470-3948463 [Escherichia coli O127:H6 str. E2348/69]
ATGGCGAATTGTCGTATTCTGTTAACGCCGTTGAATGAACGTGACGAACAGCGCGGATATTCGACACAGGGGTTGAAGCG
ACTGAGCGGCACTGCAAAATTGAATCCTCGGCTGGGGTTTACGCGAACTCAGTTTGTGCAGGAACTTCCGCGTCAACAAA
AAGGGATGAGTATTTCCGGTTACCAGCCCAAATTGCAATTAGTGCTGGATGAAGGAGAGTTCCGCGTCGTAGATCATCAG
GGAAATTTTATCCTTAAGCCCTCTCCAGCCGACTTTCCCGGTTTGGCCGAAAATGAACACGCAACAATGACATTGATGTC
TCGTTTGGGATTTGACGTACCTGTGCATGGCCTGTTGAGTTTTGCACCACAATCAGAGGAAGAGCTTGAATACGCATTTG
TTATCCGGCGCTATGACCGGGATAACAAGGGGCTGCCAGTGCATCAGGAACAGCTTGATGGCGCGATGCAGATTACAGAT
AAATATGGCAAAACGGGGAATGATAACGAACAATATGTCAGCTATGAAACGCTGGCCCGATTTTTAGTTGCCCATGTTAA
CGACAACATCGCATTTAAAATCGATCTCTTTCGTCGCATTGTTTACGCCTGGTTGCTGGGTAATAACGACATGCATTTAC
GAAACTTTGGCTTAGTGTACTCCGATGGTTTGACGCCTGCGTTAGCGCCGGTGTATGACTTTGTTTCTGTCGCTCCCTAT
CCGGAATATTTTTACTCAAACTATCTGGCGCTGCCGTTATTGACGCGAGAAGAAGGCGGTCGGGAACTGGCTCCTGGATT
TCACAGTGATTATGGCGAGTATATCGGGCAGGACTTTTTGCTGTTGGGTGAATCAATGGGGTTAGCGCCCAGATTGCTGG
AAAAACTCTTTCAGGATATACGTAAAGAAAATGCCATTGTTATGGAAACGTATGAGCAGTCCTTTATGACTCAGGATCAT
ATCCAGGCTGTGCTGCAATGCTATCGACATCGTCTGGGGCTGCTGTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11831.41 Da        Isoelectric Point: 6.3215

>AT10121 WP_001346664.1 NC_011601:c3949781-3949470 [Escherichia coli O127:H6 str. E2348/69]
MHRRVKVLLYGQVVGELSQNDSGFLFQYAHDYHGPAISISLPVAQRQFPSETLHPYFASLAPEGWLRQRYSQIQHRDEND
LLGMLIDNGKNLLGAIQILPWEE

Download         Length: 312 bp

>AT10121 NC_011601:c3949781-3949470 [Escherichia coli O127:H6 str. E2348/69]
ATGCATCGGCGAGTGAAAGTGTTGCTCTATGGTCAGGTAGTGGGTGAACTCTCTCAGAACGACAGTGGTTTTTTGTTCCA
GTACGCCCACGATTACCACGGACCGGCAATCTCAATCAGTCTGCCCGTCGCACAGCGTCAGTTTCCCAGTGAGACGTTGC
ATCCTTATTTCGCCTCACTGGCGCCAGAAGGATGGCTGCGTCAACGCTACAGCCAGATACAGCATCGTGACGAAAACGAT
CTGCTGGGAATGCTTATTGATAACGGCAAGAATCTGCTGGGGGCCATCCAGATTTTACCTTGGGAGGAATAA

Regulator


Download         Length: 108 a.a.        Molecular weight: 11837.81 Da        Isoelectric Point: 8.5240

>RE10121 WP_000563102.1 NC_011601:c3950089-3949766 [Escherichia coli O127:H6 str. E2348/69]
MICSGPQNLAQELKAIGDQLQELQKKLIQGPNISQPRPLLTIETPRHLGEQLNARRKELGIDLYTLELQTGISTSTLKRL
FKDPEQVKFGSVFAVANVLGVKLCIGE

Download         Length: 324 bp

>RE10121 NC_011601:c3950089-3949766 [Escherichia coli O127:H6 str. E2348/69]
ATGATCTGCTCAGGACCACAAAATCTGGCTCAAGAGTTGAAAGCGATCGGCGACCAACTCCAGGAGTTGCAGAAGAAACT
TATTCAGGGGCCGAATATTAGTCAACCACGCCCCCTCCTGACCATTGAGACTCCCCGTCATTTGGGGGAACAACTGAACG
CACGTCGCAAAGAATTGGGCATTGATTTATACACACTTGAACTACAAACGGGGATCTCAACGTCCACGTTAAAGCGACTT
TTTAAGGATCCTGAACAGGTTAAATTCGGCAGTGTGTTCGCGGTGGCGAATGTTCTTGGAGTGAAACTATGCATCGGCGA
GTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10036 Legionella pneumophila subsp. pneumophila str. Philadelphia 1

29.326

100

0.321

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AB4
PDB 7AB5
PDB 7AB3

Antitoxin

Source ID Structure
PDB 7AB4
PDB 7AB5
PDB 7AB3
AlphaFold DB A0A0K5V9L6

References

(1) Stine Vang Nielsen et al. (2019) Serine-Threonine Kinases Encoded by Split hipA Homologs Inhibit Tryptophanyl-tRNA Synthetase. MBio 10(3):e01138-19. [PubMed:31213559] experimental


experimental experimental literature