119028

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 572354..572856 Replicon chromosome
Accession NZ_CP035884
Organism Escherichia coli strain LSN03-016011/A

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EW995_RS02935 Protein ID WP_000767829.1
Coordinates 572354..572608 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EW995_RS02940 Protein ID WP_001259255.1
Coordinates 572605..572856 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW995_RS02910 (568190) 568190..568648 - 459 WP_000526113.1 IS200/IS605-like element IS200C family transposase -
EW995_RS02915 (568847) 568847..570205 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EW995_RS02920 (570195) 570195..570257 - 63 WP_010723108.1 membrane protein YoeI -
EW995_RS02925 (570472) 570472..571401 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
EW995_RS02930 (571447) 571447..572271 - 825 WP_000754758.1 SDR family oxidoreductase -
EW995_RS02935 (572354) 572354..572608 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EW995_RS02940 (572605) 572605..572856 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EW995_RS02945 (573139) 573139..573189 + 51 WP_001364200.1 his operon leader peptide -
EW995_RS02950 (573335) 573335..574234 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EW995_RS02955 (574240) 574240..575544 + 1305 WP_001774536.1 histidinol dehydrogenase -
EW995_RS02960 (575541) 575541..576611 + 1071 WP_001362957.1 histidinol-phosphate transaminase -
EW995_RS02965 (576611) 576611..577678 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 568190..568648 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T119028 WP_000767829.1 NZ_CP035884:c572608-572354 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T119028 NZ_CP035884:c572608-572354 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT119028 WP_001259255.1 NZ_CP035884:c572856-572605 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT119028 NZ_CP035884:c572856-572605 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References