155107

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 47284..47809 Replicon plasmid pSCU-111-1
Accession NZ_CP051728
Organism Escherichia coli strain SCU-111

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HHJ28_RS23035 Protein ID WP_001159871.1
Coordinates 47504..47809 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag HHJ28_RS23030 Protein ID WP_000813639.1
Coordinates 47284..47502 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ28_RS23005 42353..43375 + 1023 WP_000255956.1 IS21-like element IS100 family transposase -
HHJ28_RS23010 43372..44154 + 783 WP_097748551.1 IS21-like element IS100kyp family helper ATPase IstB -
HHJ28_RS23015 44198..44446 - 249 WP_169062082.1 Dabb family protein -
HHJ28_RS23020 44458..45519 - 1062 WP_106907065.1 NAD(P)-dependent alcohol dehydrogenase -
HHJ28_RS23025 45668..46627 + 960 WP_106907067.1 LysR family transcriptional regulator -
HHJ28_RS23030 47284..47502 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HHJ28_RS23035 47504..47809 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HHJ28_RS23040 47810..48616 + 807 WP_077781165.1 site-specific integrase -
HHJ28_RS23045 49174..49929 + 756 WP_169062083.1 replication initiation protein RepE -
HHJ28_RS23050 50777..51409 + 633 WP_000312330.1 ParA family protein -
HHJ28_RS23055 51409..51783 + 375 WP_042021187.1 hypothetical protein -
HHJ28_RS23060 51961..52419 + 459 WP_000526115.1 IS200/IS605-like element IS200C family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - acrB / pet / etpB / etpB / cdtC / cdtB / cdtA / etpA / etpB 1..189717 189717
flank IS/Tn - - 51961..52419 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T155107 WP_001159871.1 NZ_CP051728:47504-47809 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T155107 NZ_CP068650:c51891-51622 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTACTGTCGTGGACTGATGACGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAACAAACTCATCAATGATATTAAGCGTTCCTCATTTGAGGGCATCGGTAAACCAGAGCCATTAA
AAGAGAACTTATCTGGCTTTTGGTCTCGCCGTATTGATGACACTAATAGGCTTGTTTACGCAGTCGATGACCAAGCTATA
ACGATAATTTCGTGTCGTTATCACTACTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT155107 WP_000813639.1 NZ_CP051728:47284-47502 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT155107 NZ_CP068650:c52138-51884 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTTAAAGCTGTTTTAGACGGTGTAGTTAATGACGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAAGATGCCGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACAGTTCACT
TACTACGCTCTCCTCAAAACGCTGAACATCTAAATCGCTCGATTGCACAGTACCGTGCTGGTAAAACAACAGCACGAGAG
TTAATCGATGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References