169224

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 231132..232450 Replicon chromosome
Accession NZ_CP059840
Organism Escherichia coli strain E2348/69

Toxin (Protein)


Gene name HipT Uniprot ID B7UL96
Locus tag H4J01_RS01170 Protein ID WP_001262465.1
Coordinates 231443..232450 (+) Length 336 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID B7UL97
Locus tag H4J01_RS01165 Protein ID WP_001346664.1
Coordinates 231132..231443 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4J01_RS01140 226664..227683 + 1020 WP_000938855.1 dipeptide ABC transporter permease DppB -
H4J01_RS01145 227693..228595 + 903 WP_000084677.1 dipeptide ABC transporter permease DppC -
H4J01_RS01150 228606..229589 + 984 WP_001196477.1 dipeptide ABC transporter ATP-binding protein -
H4J01_RS01155 229586..230599 + 1014 WP_000103582.1 dipeptide ABC transporter ATP-binding subunit DppF -
H4J01_RS01160 230824..231147 + 324 WP_000563102.1 helix-turn-helix transcriptional regulator -
H4J01_RS01165 231132..231443 + 312 WP_001346664.1 HipA N-terminal domain-containing protein Antitoxin
H4J01_RS01170 231443..232450 + 1008 WP_001262465.1 HipA domain-containing protein Toxin
H4J01_RS01175 232467..233738 - 1272 WP_001339917.1 amino acid permease -
- 234100..234165 - 66 NuclAT_19 - -
- 234100..234165 - 66 NuclAT_19 - -
- 234100..234165 - 66 NuclAT_19 - -
- 234100..234165 - 66 NuclAT_19 - -
- 234100..234165 - 66 NuclAT_25 - -
- 234100..234165 - 66 NuclAT_25 - -
- 234100..234165 - 66 NuclAT_25 - -
- 234100..234165 - 66 NuclAT_25 - -
H4J01_RS01180 234214..234321 + 108 WP_000170747.1 type I toxin-antitoxin system toxin Ldr family protein -
- 234583..234648 - 66 NuclAT_18 - -
- 234583..234648 - 66 NuclAT_18 - -
- 234583..234648 - 66 NuclAT_18 - -
- 234583..234648 - 66 NuclAT_18 - -
- 234583..234648 - 66 NuclAT_24 - -
- 234583..234648 - 66 NuclAT_24 - -
- 234583..234648 - 66 NuclAT_24 - -
- 234583..234648 - 66 NuclAT_24 - -
H4J01_RS01185 234697..234804 + 108 WP_001295224.1 type I toxin-antitoxin system toxin Ldr family protein -
H4J01_RS01190 235028..235486 + 459 WP_000526113.1 IS200/IS605-like element IS200C family transposase -
H4J01_RS01195 235601..237280 - 1680 WP_000191555.1 cellulose biosynthesis protein BcsG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 235028..235486 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(56-302)

Antitoxin

HipA, N-terminal subdomain 1

(6-99)


Sequences

Toxin        


Download         Length: 336 a.a.        Molecular weight: 38329.55 Da        Isoelectric Point: 5.4135

>T169224 WP_001262465.1 NZ_CP059840:231443-232450 [Escherichia coli]
MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLNPRLGFTRTQFVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQ
GNFILKPSPADFPGLAENEHATMTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDNKGLPVHQEQLDGAMQITD
KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGLVYSDGLTPALAPVYDFVSVAPY
PEYFYSNYLALPLLTREEGGRELAPGFHSDYGEYIGQDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDH
IQAVLQCYRHRLGLL

Download         Length: 1008 bp

>T169224 NZ_CP076305:c3803084-3802788 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11831.41 Da        Isoelectric Point: 6.3215

>AT169224 WP_001346664.1 NZ_CP059840:231132-231443 [Escherichia coli]
MHRRVKVLLYGQVVGELSQNDSGFLFQYAHDYHGPAISISLPVAQRQFPSETLHPYFASLAPEGWLRQRYSQIQHRDEND
LLGMLIDNGKNLLGAIQILPWEE

Download         Length: 312 bp

>AT169224 NZ_CP076305:c3802786-3802391 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AB4
PDB 7AB5
PDB 7AB3

Antitoxin

Source ID Structure
PDB 7AB4
PDB 7AB5
PDB 7AB3
AlphaFold DB A0A0K5V9L6

References