TAfinder detailed results

Overview

Job ID wrVbGpMxFf
Sequence name NZ_CP026085.1 Escherichia coli strain DH5alpha chromosome, complete genome
Predicted type II

Toxin (Protein)


Predicted family hicA (T10189) Predicted domain COG1724 (HicA)
Locus tag orf2345 Length 58 a.a.
Coordinates 2483743..2483919 (-)

Antitoxin (Protein)


Predicted family hicB (AT10189) Predicted domain PF03681 (UPF0150)
Locus tag orf2344 Length 145 a.a.
Coordinates 2483281..2483718 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
orf2340 2478433..2479374 - 942 ORF2340 Predicted ORF by PRODIGAL Version 1.20 -
orf2341 2479375..2480388 - 1014 ORF2341 Predicted ORF by PRODIGAL Version 1.20 -
orf2342 2480406..2481551 - 1146 ORF2342 Predicted ORF by PRODIGAL Version 1.20 -
orf2343 2481796..2483202 - 1407 ORF2343 Predicted ORF by PRODIGAL Version 1.20 -
orf2344 2483281..2483718 - 438 ORF2344 Predicted ORF by PRODIGAL Version 1.20 Antitoxin
orf2345 2483743..2483919 - 177 ORF2345 Predicted ORF by PRODIGAL Version 1.20 Toxin
orf2346 2484201..2484371 + 171 ORF2346 Predicted ORF by PRODIGAL Version 1.20 -
orf2347 2484463..2486424 - 1962 ORF2347 Predicted ORF by PRODIGAL Version 1.20 -
orf2348 2486497..2487033 - 537 ORF2348 Predicted ORF by PRODIGAL Version 1.20 -
orf2349 2487125..2488300 + 1176 ORF2349 Predicted ORF by PRODIGAL Version 1.20 -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin

COG1724 (HicA) (E-value: 0.0055, Score:14.8)

(7-66)

PF07927 (HicA) (E-value: 0.011, Score:13.8)

(20-78)

Antitoxin

PF03681 (UPF0150) (E-value: 1.2e-14, Score:52.4)

(11-88)

COG1598 (HicB) (E-value: 6.5e-10, Score:37.1)

(5-128)

HicB_lk_antitox (HicB) (E-value: 2.7e-08, Score:32)

(1-65)

PF13744 (HTH_37) (E-value: 0.0035, Score:15.3)

(38-88)

PRK01381 (E-value: 0.0051, Score:14.9)

(40-74)

HicB-like_2 (HicB) (E-value: 0.017, Score:13.3)

(12-78)

PRK09726 (HipB) (E-value: 0.05, Score:11.6)

(10-44)

COG3093 (VapI) (E-value: 0.023, Score:11.5)

(14-64)

PF03681 (UPF0150) (E-value: 320, Score:-0.6)

(84-110)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>ORF2345 TA_18 Toxin
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>ORF2345 TA_18 Toxin
TTAACTCAAACCGAGTTGTTTCAGGATTGCTTTACGCAATGGTTCTTTAATCTCATCGCA
GGGGTGACGCGGCATGACACTGCGCCTCCCATGAAACCTGAGTTTCAAATGGTTGCTGCC
ATTCGCTACATCGACGCCCTGAGATTCGAGCCAACGTCTGAACTCGCTTTGTTTCAC

Antitoxin


Download         Length: 145 a.a.        Molecular weight: 16034.56 Da        Isoelectric Point: 4.6420

>ORF2344 TA_18 Antitoxin
MRGTVEIMRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFED
NELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGKPKQEITRLFDL
HHATKIDAVQLAAKALGKELSLVMV

Download         Length: 438 bp

>ORF2344 TA_18 Antitoxin
TTAAACCATCACCAGCGATAACTCTTTGCCAAGCGCCTTTGCCGCGAGCTGGACGGCGTC
GATTTTTGTCGCATGATGCAAGTCAAATAGGCGAGTAATCTCCTGTTTAGGTTTGCCAAT
TCGCCTGGCTAACTCTTGCTGAGTGATTTCTGACTGTAAAAAAGCATTTAACAGCAATAC
CTTAGAGGCGACGCTCAAAGGTACTTCAATAAAGTGATCATGACTATTTAATGGCGAAGG
TAAAGGGATAAGCTCGTTATCTTCAAAATAAAAATCAAATGCGGTCAGTAAAGCATCTTT
TGCCGCTTCCATCGCTTCAGCGACAGTTTCGCCCTGCGTCAACGCTTCAGGGATATCCAC
AAAAGAAACCATATAACCGCCTTCCGGCGCGGGTGTAAGAGTGACGGGATAACGCATAAT
TTCGACTGTCCCTCGCAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10189 Escherichia coli strain DH5alpha

100

100

1
T1039 Escherichia coli str. K-12 substr. MG1655

100

100

1
T6283 Pseudomonas aeruginosa PA1

47.458

98.333

0.483
Protein Organism Identities (%) Coverage (%) Ha-value
AT10189 Escherichia coli strain DH5alpha

99.275

100

0.945
AT1039 Escherichia coli str. K-12 substr. MG1655

99.275

100

0.945
AT6283 Pseudomonas aeruginosa PA1

30.833

50.209

0.255
AT3593 Acinetobacter baumannii ATCC 17978

28.302

73.611

0.207
AT10168 Streptococcus pneumoniae TIGR4

38.983

39.333

0.159
AT875 Sinorhizobium meliloti 1021

38.462

33.987

0.138
AT6107 Burkholderia pseudomallei K96243

27.273

31.884

0.083