TAfinder detailed results

Overview

Job ID wrVbGpMxFf
Sequence name NZ_CP026085.1 Escherichia coli strain DH5alpha chromosome, complete genome
Predicted type II

Toxin (Protein)


Predicted family tad (T6025) Predicted domain COG4679 (Tad)
Locus tag orf1705 Length 112 a.a.
Coordinates 1819807..1820145 (-)

Antitoxin (Protein)


Predicted family ata (AT6025) Predicted domain COG5606 (Ata)
Locus tag orf1704 Length 96 a.a.
Coordinates 1819527..1819817 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
orf1701 1816285..1816926 + 642 ORF1701 Predicted ORF by PRODIGAL Version 1.20 -
orf1702 1817018..1817599 + 582 ORF1702 Predicted ORF by PRODIGAL Version 1.20 -
orf1703 1817621..1819474 + 1854 ORF1703 Predicted ORF by PRODIGAL Version 1.20 -
orf1704 1819527..1819817 - 291 ORF1704 Predicted ORF by PRODIGAL Version 1.20 Antitoxin
orf1705 1819807..1820145 - 339 ORF1705 Predicted ORF by PRODIGAL Version 1.20 Toxin
orf1706 1820220..1821803 - 1584 ORF1706 Predicted ORF by PRODIGAL Version 1.20 -
orf1707 1822462..1823601 + 1140 ORF1707 Predicted ORF by PRODIGAL Version 1.20 -
orf1708 1823607..1824050 + 444 ORF1708 Predicted ORF by PRODIGAL Version 1.20 -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin

COG4679 (Tad) (E-value: 1.3e-51, Score:171.6)

(6-116)

PF05973 (Gp49) (E-value: 4.4e-43, Score:143.6)

(4-111)

TIGR02683 (upstrm_HI1419) (E-value: 1.7e-07, Score:29.4)

(22-107)

COG3657 (E-value: 2.1e-07, Score:28.8)

(26-106)

COG2026 (RelE) (E-value: 0.0014, Score:16.8)

(3-84)

Antitoxin

COG5606 (Ata) (E-value: 4.8e-41, Score:136.7)

(2-95)

PF13744 (HTH_37) (E-value: 6.5e-12, Score:43.3)

(8-88)

TIGR03070 (couple_hipB) (E-value: 1e-05, Score:22.9)

(217-280)

PF13560 (HTH_31) (E-value: 0.00069, Score:16.9)

(25-56)

smart00530 (HTH_XRE) (E-value: 0.0014, Score:16.8)

(17-53)

cd00093 (Xre) (E-value: 0.0092, Score:13.4)

(46-107)

YdaS_antitoxin (YdaS) (E-value: 0.028, Score:12.5)

(10-35)

TIGR02607 (HigA) (E-value: 0.042, Score:11.7)

(13-49)

YdaS_antitoxin (YdaS) (E-value: 270, Score:-0.3)

(25-30)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 13251.03 Da        Isoelectric Point: 9.3193

>ORF1705 TA_14 Toxin
MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKPFSTIGPGVREIRTRDA
DGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYKELVQERKNEN

Download         Length: 339 bp

>ORF1705 TA_14 Toxin
TCAATTTTCATTTTTCCGCTCCTGAACAAGTTCTTTATAGCGTCGTTTGGCTAAATCAAT
ATCCGATTGACTGGTAGTCTGTGTTTTCTTTTGAAAACAATGCAATACGTAAACCGCTTC
TTCAAATTTTGCGACATACATGACGCGATAGATCCCATCAGCATCGCGAGTACGAATCTC
TCGCACACCAGGGCCGATAGTTGAAAAAGGTTTCCAGTCGTATGGGTTTAACCCTTGTTG
GATCCTGTCGAGTTGATAGCCAATTTCCTGTCGAGACGATTCTGGAAATGCGCGTAAATC
GTCAAGACTGGTATCAAGAAAAGCCAGCTTTTTTCTCAT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10790.35 Da        Isoelectric Point: 5.5675

>ORF1704 TA_14 Antitoxin
MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQAQAASALGVTQPRISE
LARGKIQIFSIDKLITMMAHAGLHIQRIEIQYPHAA

Download         Length: 291 bp

>ORF1704 TA_14 Antitoxin
CTAAGCAGCATGTGGATATTGAATTTCAATACGTTGAATATGCAATCCTGCATGAGCCAT
CATGGTGATCAATTTATCGATACTAAAGATCTGGATCTTACCTCTGGCAAGTTCAGATAT
GCGAGGTTGAGTGACCCCTAAAGCAGAAGCTGCCTGAGCCTGGCTAAACCCTTGTTGTTC
AATCCAGTTATTAATGATCGTTACAAGCTCGGCACGGATCCGCATATTTTCCGCTTGTTC
AGGGGTGTCACTCACCGCATCCCACACACTATCAAAGGTTTCAATTTTCAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6025 Pseudomonas sp. LM13

40

92

0.411
T6038 Paracoccus aminophilus

37.607

93.6

0.393
T10037 Burkholderia pseudomallei K96243

32.967

82.727

0.268
T325 Streptococcus pneumoniae TIGR4

37.5

39.669

0.161
T1067 Haemophilus influenzae Rd KW20

31.169

64.706

0.214
Protein Organism Identities (%) Coverage (%) Ha-value
AT6025 Pseudomonas sp. LM13

31.343

60.36

0.219
AT10075 Caulobacter crescentus NA1000

33.333

20.07

0.198
AT127 Mycobacterium tuberculosis H37Rv

32.558

39.45

0.146