Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/- |
| Location | 3966098..3966755 | Replicon | chromosome |
| Accession | NC_006350 | ||
| Organism | Burkholderia pseudomallei K96243 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A0F6L6G8 |
| Locus tag | BPS_RS18030 | Protein ID | WP_009910242.1 |
| Coordinates | 3966423..3966755 (-) | Length | 111 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | BPS_RS18025 | Protein ID | WP_009938955.1 |
| Coordinates | 3966098..3966415 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BPS_RS18000 (BPSL3340) | 3962264..3962908 | - | 645 | WP_004195754.1 | lytic polysaccharide monooxygenase | - |
| BPS_RS18005 (BPSL3341) | 3963415..3963732 | - | 318 | WP_004266306.1 | hypothetical protein | - |
| BPS_RS18010 | 3963949..3964173 | + | 225 | WP_004195757.1 | hypothetical protein | - |
| BPS_RS18020 (BPSL3342) | 3964455..3966017 | - | 1563 | WP_009938954.1 | ATP-binding protein | - |
| BPS_RS18025 | 3966098..3966415 | - | 318 | WP_009938955.1 | helix-turn-helix domain-containing protein | Antitoxin |
| BPS_RS18030 (BPSL3343) | 3966423..3966755 | - | 333 | WP_009910242.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| BPS_RS18035 (BPSL3344) | 3966797..3967540 | - | 744 | WP_162486671.1 | site-specific integrase | - |
| BPS_RS18040 (BPSL3345) | 3967732..3968511 | - | 780 | WP_011205299.1 | bacteriophage-like protein | - |
| BPS_RS18045 (BPSL3346) | 3968968..3969561 | - | 594 | WP_009938959.1 | hypothetical protein | - |
| BPS_RS18050 (BPSL3347) | 3969661..3971037 | - | 1377 | WP_011205301.1 | bacteriophage-related lipoprotein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Prophage | - | - | 3963415..3974993 | 11578 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 111 a.a. Molecular weight: 12849.92 Da Isoelectric Point: 10.3619
>T10037 WP_009910242.1 NC_006350:c3966755-3966423 [Burkholderia pseudomallei K96243]
MKFDWTVVYYNERVKRDVFAFPAGILADYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPKGREGIGRVFYCTHVGRQVV
VLHSFVKKTQETPQHELRIAQARLREVRNG
MKFDWTVVYYNERVKRDVFAFPAGILADYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPKGREGIGRVFYCTHVGRQVV
VLHSFVKKTQETPQHELRIAQARLREVRNG
Download Length: 333 bp
>T10037 NC_006350:c3966755-3966423 [Burkholderia pseudomallei K96243]
ATGAAATTCGATTGGACGGTTGTCTATTACAACGAACGGGTCAAGCGCGACGTTTTCGCGTTCCCGGCTGGGATTCTTGC
GGACTACCTCCGGCTGTTGGATCTGATGCAGGAGTTCGGGGCGGATCTACGGATGCCGCACTCCCGGGCAATGGGTAACG
GGTTGTTTGAGTTGCGTCCAAAGGGTAGGGAAGGTATCGGGCGGGTGTTCTATTGCACGCATGTGGGGCGGCAAGTCGTC
GTGCTGCACTCCTTCGTCAAGAAAACACAGGAAACGCCGCAACATGAATTGCGGATCGCCCAAGCACGTTTGAGAGAGGT
GCGTAATGGCTAA
ATGAAATTCGATTGGACGGTTGTCTATTACAACGAACGGGTCAAGCGCGACGTTTTCGCGTTCCCGGCTGGGATTCTTGC
GGACTACCTCCGGCTGTTGGATCTGATGCAGGAGTTCGGGGCGGATCTACGGATGCCGCACTCCCGGGCAATGGGTAACG
GGTTGTTTGAGTTGCGTCCAAAGGGTAGGGAAGGTATCGGGCGGGTGTTCTATTGCACGCATGTGGGGCGGCAAGTCGTC
GTGCTGCACTCCTTCGTCAAGAAAACACAGGAAACGCCGCAACATGAATTGCGGATCGCCCAAGCACGTTTGAGAGAGGT
GCGTAATGGCTAA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11250.76 Da Isoelectric Point: 9.5977
>AT10037 WP_009938955.1 NC_006350:c3966415-3966098 [Burkholderia pseudomallei K96243]
VKRVRAEGFNPIPHTADDTARLLADSKVKAAYDALEDEYSALRAILAARQEAGLTQAQVAERMGTTASAVSRLEASLTSE
KHSPSFATLRKYAAACGKRLVISFA
VKRVRAEGFNPIPHTADDTARLLADSKVKAAYDALEDEYSALRAILAARQEAGLTQAQVAERMGTTASAVSRLEASLTSE
KHSPSFATLRKYAAACGKRLVISFA
Download Length: 318 bp
>AT10037 NC_006350:c3966415-3966098 [Burkholderia pseudomallei K96243]
GTGAAGCGTGTTCGTGCTGAGGGATTCAATCCAATTCCGCATACCGCAGACGACACAGCTCGTCTGCTCGCAGATAGTAA
GGTCAAGGCGGCCTACGATGCGCTGGAAGATGAATACTCGGCATTGCGCGCTATATTGGCCGCTCGGCAAGAGGCTGGAT
TGACTCAAGCGCAGGTCGCTGAGCGTATGGGTACGACGGCGTCAGCCGTGTCGCGCCTTGAAGCATCGCTAACTAGCGAA
AAGCATTCGCCTTCGTTTGCGACGCTGCGAAAGTATGCGGCTGCATGTGGTAAGAGGCTTGTGATTTCGTTCGCTTGA
GTGAAGCGTGTTCGTGCTGAGGGATTCAATCCAATTCCGCATACCGCAGACGACACAGCTCGTCTGCTCGCAGATAGTAA
GGTCAAGGCGGCCTACGATGCGCTGGAAGATGAATACTCGGCATTGCGCGCTATATTGGCCGCTCGGCAAGAGGCTGGAT
TGACTCAAGCGCAGGTCGCTGAGCGTATGGGTACGACGGCGTCAGCCGTGTCGCGCCTTGAAGCATCGCTAACTAGCGAA
AAGCATTCGCCTTCGTTTGCGACGCTGCGAAAGTATGCGGCTGCATGTGGTAAGAGGCTTGTGATTTCGTTCGCTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Itziar Chapartegui-González et al. (2022) Evaluating the Contribution of the Predicted Toxin-Antitoxin System HigBA to Persistence, Biofilm Formation, and Virulence in Burkholderia pseudomallei. Infection And Immunity 90(7):e0003522. [PubMed:35695502]