Detailed information of TA system TA049227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1812616..1813273
• Replicon: chromosome
• Accession: NZ_CP009538
• Organism: Burkholderia pseudomallei K96243

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0F6L6G8
• Locus tag: AQ15_RS21110
• Protein ID: WP_009910242.1
• Coordinates: 1812616..1812948 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AQ15_RS36865
• Protein ID: WP_009938955.1
• Coordinates: 1812956..1813273 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQ15_RS21090	1808334..1809710	+	1377	WP_011205301.1	bacteriophage-related lipoprotein	-
AQ15_RS21095	1809810..1810403	+	594	WP_009938959.1	hypothetical protein	-
AQ15_RS21100	1810860..1811639	+	780	WP_011205299.1	bacteriophage-like protein	-
AQ15_RS21105	1811831..1812574	+	744	WP_162486671.1	site-specific integrase	-
AQ15_RS21110	1812616..1812948	+	333	WP_009910242.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AQ15_RS36865	1812956..1813273	+	318	WP_009938955.1	helix-turn-helix domain-containing protein	Antitoxin
AQ15_RS21120	1813354..1814916	+	1563	WP_009938954.1	ATP-binding protein	-
AQ15_RS36870	1815198..1815422	-	225	WP_004195757.1	hypothetical protein	-
AQ15_RS21130	1815639..1815956	+	318	WP_004266306.1	hypothetical protein	-
AQ15_RS21135	1816463..1817107	+	645	WP_004195754.1	lytic polysaccharide monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_006350 Burkholderia pseudomallei K96243 Prophage TA010037 3963415..3974993 0	inside	Prophage	-	-	1804378..1815956	11578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-100)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12849.92 Da
• Isoelectric Point: 10.3619

>T49227 WP_009910242.1 NZ_CP009538:1812616-1812948 [Burkholderia pseudomallei K96243]
MKFDWTVVYYNERVKRDVFAFPAGILADYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPKGREGIGRVFYCTHVGRQVV
VLHSFVKKTQETPQHELRIAQARLREVRNG

Nucleotide

• Length: 333 bp

>T49227 NZ_CP009538:1812616-1812948 [Burkholderia pseudomallei K96243]
ATGAAATTCGATTGGACGGTTGTCTATTACAACGAACGGGTCAAGCGCGACGTTTTCGCGTTCCCGGCTGGGATTCTTGC
GGACTACCTCCGGCTGTTGGATCTGATGCAGGAGTTCGGGGCGGATCTACGGATGCCGCACTCCCGGGCAATGGGTAACG
GGTTGTTTGAGTTGCGTCCAAAGGGTAGGGAAGGTATCGGGCGGGTGTTCTATTGCACGCATGTGGGGCGGCAAGTCGTC
GTGCTGCACTCCTTCGTCAAGAAAACACAGGAAACGCCGCAACATGAATTGCGGATCGCCCAAGCACGTTTGAGAGAGGT
GCGTAATGGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11250.76 Da
• Isoelectric Point: 9.5977

>AT49227 WP_009938955.1 NZ_CP009538:1812956-1813273 [Burkholderia pseudomallei K96243]
VKRVRAEGFNPIPHTADDTARLLADSKVKAAYDALEDEYSALRAILAARQEAGLTQAQVAERMGTTASAVSRLEASLTSE
KHSPSFATLRKYAAACGKRLVISFA

Nucleotide

• Length: 318 bp

>AT49227 NZ_CP009538:1812956-1813273 [Burkholderia pseudomallei K96243]
GTGAAGCGTGTTCGTGCTGAGGGATTCAATCCAATTCCGCATACCGCAGACGACACAGCTCGTCTGCTCGCAGATAGTAA
GGTCAAGGCGGCCTACGATGCGCTGGAAGATGAATACTCGGCATTGCGCGCTATATTGGCCGCTCGGCAAGAGGCTGGAT
TGACTCAAGCGCAGGTCGCTGAGCGTATGGGTACGACGGCGTCAGCCGTGTCGCGCCTTGAAGCATCGCTAACTAGCGAA
AAGCATTCGCCTTCGTTTGCGACGCTGCGAAAGTATGCGGCTGCATGTGGTAAGAGGCTTGTGATTTCGTTCGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0F6L6G8