Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tad-ata/COG5606-RelE |
| Location | 3309..4002 | Replicon | plasmid pAMI2 |
| Accession | NC_010847 | ||
| Organism | Paracoccus aminophilus | ||
Toxin (Protein)
| Gene name | tad | Uniprot ID | A2T3W3 |
| Locus tag | G7C88_RS00030 | Protein ID | WP_012476881.1 |
| Coordinates | 3309..3686 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | ata | Uniprot ID | - |
| Locus tag | G7C88_RS00035 | Protein ID | WP_012476882.1 |
| Coordinates | 3667..4002 (+) | Length | 112 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G7C88_RS00005 (pAMI2_p01) | 67..630 | + | 564 | WP_012476877.1 | recombinase family protein | - |
| G7C88_RS00010 | 721..936 | + | 216 | WP_168550538.1 | ribbon-helix-helix protein, CopG family | - |
| G7C88_RS00015 (pAMI2_p02) | 1127..2002 | + | 876 | WP_012476878.1 | helix-turn-helix domain-containing protein | - |
| G7C88_RS00020 (pAMI2_p03) | 2231..2890 | + | 660 | WP_012476879.1 | AAA family ATPase | - |
| G7C88_RS00025 (pAMI2_p04) | 2887..3225 | + | 339 | WP_012476880.1 | chromosome partitioning protein ParB | - |
| G7C88_RS00030 (pAMI2_p05) | 3309..3686 | + | 378 | WP_012476881.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| G7C88_RS00035 (pAMI2_p06) | 3667..4002 | + | 336 | WP_012476882.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| G7C88_RS00040 | 4089..4205 | + | 117 | Protein_7 | transcriptional regulator | - |
| G7C88_RS00045 | 4422..4646 | - | 225 | WP_238829642.1 | hypothetical protein | - |
| G7C88_RS00050 (pAMI2_p08) | 4597..5538 | - | 942 | Protein_9 | type IV secretory system conjugative DNA transfer family protein | - |
| G7C88_RS00055 | 5472..6152 | - | 681 | WP_168550539.1 | hypothetical protein | - |
| G7C88_RS00060 (pAMI2_p10) | 6157..6486 | - | 330 | WP_012476886.1 | hypothetical protein | - |
| G7C88_RS00065 | 6467..6667 | - | 201 | WP_168550540.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..18563 | 18563 | |
| - | flank | IS/Tn | - | - | 67..630 | 563 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13884.94 Da Isoelectric Point: 7.4733
>T6038 WP_012476881.1 NC_010847:3309-3686 [Paracoccus aminophilus JCM 7686]
MGQDERTLEWIGSSYKDLMELPADVRSFIGFALHFAQHGEQHPAAKVLKGFGGAGVLEVVENDVGGTYRAVYTVKFEEAV
FVLHCFQKKSKSGIATPKEDMDVIRARLKVAEALAKEMKNAHPHR
MGQDERTLEWIGSSYKDLMELPADVRSFIGFALHFAQHGEQHPAAKVLKGFGGAGVLEVVENDVGGTYRAVYTVKFEEAV
FVLHCFQKKSKSGIATPKEDMDVIRARLKVAEALAKEMKNAHPHR
Download Length: 378 bp
>T6038 NC_010847:3309-3686 [Paracoccus aminophilus JCM 7686]
ATGGGTCAAGACGAACGAACACTGGAATGGATTGGCAGTAGCTACAAAGACTTGATGGAGCTTCCGGCCGATGTGCGGAG
TTTCATAGGGTTCGCGTTGCATTTCGCGCAGCACGGCGAGCAGCACCCAGCGGCGAAGGTGCTCAAAGGCTTCGGCGGCG
CTGGCGTTCTGGAAGTGGTTGAGAACGACGTGGGCGGCACATACCGAGCTGTTTATACGGTCAAGTTTGAGGAAGCGGTC
TTCGTGCTTCATTGCTTCCAGAAAAAGAGCAAGAGCGGCATCGCCACGCCGAAGGAAGACATGGACGTTATCCGGGCGAG
GCTCAAGGTGGCCGAGGCTCTGGCAAAGGAGATGAAAAATGCGCACCCGCATCGTTGA
ATGGGTCAAGACGAACGAACACTGGAATGGATTGGCAGTAGCTACAAAGACTTGATGGAGCTTCCGGCCGATGTGCGGAG
TTTCATAGGGTTCGCGTTGCATTTCGCGCAGCACGGCGAGCAGCACCCAGCGGCGAAGGTGCTCAAAGGCTTCGGCGGCG
CTGGCGTTCTGGAAGTGGTTGAGAACGACGTGGGCGGCACATACCGAGCTGTTTATACGGTCAAGTTTGAGGAAGCGGTC
TTCGTGCTTCATTGCTTCCAGAAAAAGAGCAAGAGCGGCATCGCCACGCCGAAGGAAGACATGGACGTTATCCGGGCGAG
GCTCAAGGTGGCCGAGGCTCTGGCAAAGGAGATGAAAAATGCGCACCCGCATCGTTGA
Antitoxin
Download Length: 112 a.a. Molecular weight: 12144.15 Da Isoelectric Point: 9.8592
>AT6038 WP_012476882.1 NC_010847:3667-4002 [Paracoccus aminophilus JCM 7686]
MRTRIVDGIEVTTTSGNVFADLVLPDAEKLKVKSGLMIEITKAVRRLDLTQEEAGRRMGLPQPKVSSLLRGDFSNLSERK
LMDALNRLGYDIEITVRPTAGPVGHLTLAVA
MRTRIVDGIEVTTTSGNVFADLVLPDAEKLKVKSGLMIEITKAVRRLDLTQEEAGRRMGLPQPKVSSLLRGDFSNLSERK
LMDALNRLGYDIEITVRPTAGPVGHLTLAVA
Download Length: 336 bp
>AT6038 NC_010847:3667-4002 [Paracoccus aminophilus JCM 7686]
ATGCGCACCCGCATCGTTGATGGGATCGAGGTCACGACCACCTCCGGCAATGTATTTGCTGATCTGGTGTTGCCCGACGC
TGAAAAGCTGAAAGTGAAATCTGGCCTGATGATCGAAATCACCAAGGCTGTGCGGCGGCTTGATCTCACGCAGGAGGAAG
CCGGGCGGCGCATGGGGCTTCCGCAGCCAAAGGTGTCGTCGCTTCTGCGCGGGGATTTCAGTAACCTGTCCGAGCGCAAG
CTAATGGACGCTCTGAACCGCCTCGGCTATGACATTGAAATCACTGTGCGGCCCACCGCTGGTCCGGTCGGGCATCTGAC
GCTTGCTGTCGCGTGA
ATGCGCACCCGCATCGTTGATGGGATCGAGGTCACGACCACCTCCGGCAATGTATTTGCTGATCTGGTGTTGCCCGACGC
TGAAAAGCTGAAAGTGAAATCTGGCCTGATGATCGAAATCACCAAGGCTGTGCGGCGGCTTGATCTCACGCAGGAGGAAG
CCGGGCGGCGCATGGGGCTTCCGCAGCCAAAGGTGTCGTCGCTTCTGCGCGGGGATTTCAGTAACCTGTCCGAGCGCAAG
CTAATGGACGCTCTGAACCGCCTCGGCTATGACATTGAAATCACTGTGCGGCCCACCGCTGGTCCGGTCGGGCATCTGAC
GCTTGCTGTCGCGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6025 | Pseudomonas sp. LM13 |
75 |
99.2 |
0.744 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6025 | Pseudomonas sp. LM13 |
70.27 |
100 |
0.703 |
Multiple sequence alignment
References
(1) Lukasz Dziewit et al. (2007) The SXT conjugative element and linear prophage N15 encode toxin-antitoxin-stabilizing systems homologous to the tad-ata module of the Paracoccus aminophilus plasmid pAMI2. Journal of Bacteriology 189(5):1983-97. [PubMed:17158670]