Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-RelE
Location 3840..4533 Replicon transposon
Accession KJ920396
Organism Pseudomonas sp. LM13

Toxin (Protein)


Gene name tad Uniprot ID -
Locus tag Locus_2 Protein ID AIL83788.1
Coordinates 3840..4217 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID -
Locus tag Locus_3 Protein ID AIL83789.1
Coordinates 4198..4533 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Locus_0 33..3062 - 3030 AIL83786.1 putative transposase -
Locus_1 3046..3681 - 636 AIL83787.1 putative resolvase -
Locus_2 3840..4217 + 378 AIL83788.1 putative toxin of addiction system Toxin
Locus_3 4198..4533 + 336 AIL83789.1 putative antitoxin of addiction system Antitoxin
Locus_4 4548..4883 + 336 AIL83790.1 hypothetical protein -
Locus_5 4907..5233 + 327 AIL83791.1 hypothetical protein -
Locus_6 5230..5610 + 381 AIL83792.1 hypothetical protein -
Locus_7 5767..7029 + 1263 AIL83793.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T6025 AIL83788.1 KJ920396:3840-4217 [Pseudomonas sp. LM13]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T6025 KJ920396:3840-4217 [Pseudomonas sp. LM13]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT6025 AIL83789.1 KJ920396:4198-4533 [Pseudomonas sp. LM13]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT6025 KJ920396:4198-4533 [Pseudomonas sp. LM13]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6038 Paracoccus aminophilus

75

99.2

0.744

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT6038 Paracoccus aminophilus

70.27

100

0.703

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References

(1) Magdalena Szuplewska et al. (2014) Mobility and generation of mosaic non-autonomous transposons by Tn3-derived inverted-repeat miniature elements (TIMEs). PloS One 9(8):e105010. [PubMed:25121765] in_silico


in_silico in silico analysis literature