Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) hipBA/-
Location 2977478..2979663 Replicon chromosome
Accession NC_011916
Organism Caulobacter crescentus NA1000

Toxin (Protein)


Gene name HipA2 Uniprot ID A0A0H3CA27
Locus tag CCNA_02821 Protein ID YP_002518194.1
Coordinates 2977478..2978812 (-) Length 445 a.a.

Antitoxin (Protein)


Gene name HipB2 Uniprot ID A0A0H3CAT0
Locus tag CCNA_02822 Protein ID YP_002518195.1
Coordinates 2978809..2979663 (-) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCNA_02816 2973537..2973908 - 372 YP_002518189.1 hypothetical protein -
CCNA_02817 2973979..2974182 - 204 YP_002518190.2 hypothetical protein -
CCNA_02818 2974861..2974974 + 114 YP_002518191.1 hypothetical protein -
CCNA_02819 2975200..2975355 - 156 YP_002518192.1 hypothetical protein -
CCNA_02820 2975365..2977254 + 1890 YP_002518193.1 TadG-family protein -
CCNA_02821 2977478..2978812 - 1335 YP_002518194.1 hipA-related phosphatidylinositol 3/4-kinase Toxin
CCNA_02822 2978809..2979663 - 855 YP_002518195.1 MerR-family transcriptional regulator Antitoxin
CCNA_03978 2979670..2979816 - 147 YP_009020550.1 hypothetical protein -
CCNA_03979 2979690..2979911 + 222 YP_009020551.1 hypothetical protein -
CCNA_02823 2979912..2980037 - 126 YP_002518196.1 hypothetical protein -
CCNA_02824 2980222..2980575 + 354 YP_002518197.1 hypothetical protein -
CCNA_02825 2980586..2980978 + 393 YP_002518198.1 transposase -
CCNA_02826 2981109..2982131 + 1023 YP_002518199.1 transposase -
CCNA_02827 2982256..2982522 + 267 YP_002518200.1 transposase -
CCNA_02828 2982516..2983442 + 927 YP_002518201.1 transposase -
CCNA_02829 2983414..2984598 + 1185 YP_002518202.1 peptidase S8 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2967624..2982522 14898


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(155-387)

Antitoxin

Cro/C1-type helix-turn-helix domain

(225-279)


Sequences

Toxin        


Download         Length: 445 a.a.        Molecular weight: 48396.41 Da        Isoelectric Point: 5.2025

>T10075 YP_002518194.1 NC_011916:c2978812-2977478 [Caulobacter vibrioides NA1000]
MSGPAGLIVRMDGFNLPAGYLASDEARAISFAYDDRYIAAGGPPLSLSMPLEQVSFGDVTARAFFDNLLPENDQMQRVMD
REGLARDDIVGLLSHLGADCSGAISCLPIGADPIKVPGVLSEDYELLAPGAIAEIARSLAERQRLPDTITDPSPVAGVQR
KIALTHTPQGFAKPRPGRKVPTTHILKVPETRLRRDARLEAAAARLASALGLDVSIPEAIVIDGVDALLITRFDRVVRDG
VVYRLHQEDFAQAMGLPATLKYQRNGAPGRQFDAQAIARVLDQTEAPALSRTAFLSATIFNLLIGNTDNHAKNHGLLYRQ
GRAPILAPLYDLLPSRMNLDFNDQLSFNIGAADHPDAITFDDMMAFFEVFGMRRAAAARFIENVIKPMIEALERATLGLA
GERLKDMDDLIGRETEQLVEVLGLDVAVRERDYYPKVRHALAPS

Download         Length: 1335 bp

>T10075 NC_011916:c2978812-2977478 [Caulobacter vibrioides NA1000]
ATGAGTGGGCCCGCAGGCCTGATCGTCCGCATGGACGGTTTTAATCTACCTGCTGGCTACCTCGCGTCGGACGAGGCGCG
GGCCATCAGCTTCGCTTACGACGACCGTTACATTGCTGCGGGCGGGCCGCCTTTGTCGCTATCCATGCCGCTTGAACAGG
TAAGCTTCGGCGATGTGACAGCGCGGGCCTTTTTCGACAACCTTCTCCCTGAAAACGATCAGATGCAGCGGGTGATGGAT
CGTGAGGGGCTGGCGCGGGACGATATCGTCGGCCTGTTAAGCCATCTAGGGGCTGACTGCTCAGGAGCGATTTCCTGTCT
GCCGATTGGCGCTGATCCCATCAAGGTTCCCGGCGTGTTGTCGGAGGACTACGAATTGCTGGCGCCGGGCGCCATCGCGG
AAATCGCCCGCAGTCTCGCCGAGCGTCAACGCCTTCCCGACACCATAACCGATCCATCCCCGGTCGCCGGAGTCCAGCGC
AAGATTGCTTTGACCCATACCCCTCAGGGGTTCGCCAAGCCTAGGCCGGGCCGCAAGGTGCCCACCACTCATATTCTAAA
GGTGCCTGAGACACGGCTGCGCCGCGATGCTCGTTTGGAGGCCGCCGCCGCGAGGTTGGCCAGTGCTCTTGGTCTGGACG
TGTCGATCCCCGAGGCCATTGTCATTGATGGCGTGGATGCTTTGCTAATCACACGTTTTGATCGAGTGGTGCGAGACGGC
GTGGTCTACCGCCTTCATCAGGAAGACTTTGCTCAGGCTATGGGGTTGCCTGCGACTTTGAAGTATCAGCGTAACGGAGC
GCCCGGTCGGCAGTTTGACGCGCAGGCCATCGCGCGCGTCCTTGACCAAACGGAGGCGCCTGCGCTGTCGCGCACGGCCT
TTCTGTCGGCGACGATCTTCAATCTTCTGATTGGCAATACTGACAACCACGCCAAGAACCATGGATTGCTCTACCGGCAA
GGTCGGGCTCCGATTCTCGCGCCGCTCTACGACCTGCTGCCCAGTCGCATGAATCTGGATTTCAATGACCAGCTGTCTTT
CAACATCGGCGCGGCTGATCACCCCGATGCGATCACCTTTGATGACATGATGGCGTTTTTTGAGGTGTTCGGCATGAGGC
GAGCGGCGGCGGCGCGCTTCATCGAAAACGTCATCAAACCCATGATCGAGGCGTTGGAACGCGCCACCTTGGGGTTGGCG
GGCGAGCGCCTCAAGGACATGGACGACCTAATCGGTCGGGAAACCGAGCAACTCGTCGAAGTGTTGGGTCTGGATGTCGC
TGTCCGCGAGAGAGACTATTACCCCAAGGTCCGCCACGCCTTGGCGCCGAGTTGA

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 28530.71 Da        Isoelectric Point: 10.0342

>AT10075 YP_002518195.1 NC_011916:c2979663-2978809 [Caulobacter vibrioides NA1000]
MLLSGSENVSDDNKGKLGIGREASVPSAADLLKRAMGGSLARDALKIAGVSGATSELLASIGRIGVASDALKMAGITSVA
SDALKIAGVGAASDALRVVSAGGLKPEFLASIGKISAASDALKLASVTSAADILKNADVGRLNAIAPPSISLASAAGGAR
QGGELSPLSDILDATRHLTNERYAVTAPSPSAADSLKQTDGGAQYNLPIARMQVPQPIKSPRALGQLVRQARERMGLTQA
AFADAAGVGRRFVSELENGKPTLELGKVMAVCAAAGLDLMAAPR

Download         Length: 855 bp

>AT10075 NC_011916:c2979663-2978809 [Caulobacter vibrioides NA1000]
ATGCTCCTGAGCGGGAGCGAAAACGTGTCTGACGATAACAAAGGAAAGCTCGGCATTGGTCGTGAGGCGAGCGTGCCTAG
TGCTGCTGACCTGCTCAAGCGCGCCATGGGAGGTTCACTCGCCCGTGACGCCCTGAAAATCGCAGGCGTTAGTGGCGCGA
CGTCCGAACTCCTGGCGTCCATCGGTAGGATCGGCGTCGCGTCCGACGCACTGAAGATGGCCGGGATTACGTCCGTGGCC
AGCGATGCGTTGAAAATCGCAGGTGTTGGCGCTGCATCCGACGCCTTGAGGGTCGTCAGCGCCGGTGGGTTAAAGCCTGA
GTTTCTGGCGTCCATCGGTAAAATCAGCGCCGCTTCCGATGCGCTCAAGCTTGCTAGCGTCACCTCTGCCGCCGACATTC
TCAAGAACGCGGACGTGGGGCGATTGAACGCTATCGCGCCTCCGTCGATCAGTTTGGCCAGCGCCGCGGGTGGCGCGCGT
CAGGGGGGGGAATTATCGCCCCTAAGCGACATTCTGGACGCAACGCGTCACCTGACCAACGAGCGCTACGCCGTGACCGC
CCCATCGCCGAGTGCGGCCGACAGCCTCAAGCAGACCGACGGGGGGGCGCAGTACAACCTGCCCATAGCGCGCATGCAGG
TGCCGCAGCCGATCAAGTCGCCGCGAGCTTTGGGTCAACTGGTCCGCCAGGCGCGCGAAAGGATGGGTTTAACCCAAGCG
GCTTTCGCTGACGCCGCAGGCGTCGGGCGTCGGTTCGTGTCGGAGCTTGAAAACGGCAAGCCGACCTTGGAGCTGGGCAA
GGTGATGGCCGTCTGCGCTGCGGCCGGCCTAGATCTGATGGCGGCGCCCAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10104 Caulobacter crescentus NA1000

35.351

97.636

0.345

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10104 Caulobacter crescentus NA1000

62.903

76.543

0.481

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3CA27

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3CAT0

References

(1) Charlie Y Huang et al. (2020) hipBA toxin-antitoxin systems mediate persistence in Caulobacter crescentus. Scientific Reports 10(1):2865. [PubMed:32071324] experimental

(2) Xiaofeng Zhou et al. (2021) A Bacterial Toxin Perturbs Intracellular Amino Acid Balance To Induce Persistence. MBio 12(1):e03020-20. [PubMed:33622732] experimental


experimental experimental literature