Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hipBA/- |
| Location | 497352..498855 | Replicon | chromosome |
| Accession | NC_011916 | ||
| Organism | Caulobacter crescentus NA1000 | ||
Toxin (Protein)
| Gene name | HipA1 | Uniprot ID | A0A0H3C5Z1 |
| Locus tag | CCNA_00482 | Protein ID | YP_002515855.1 |
| Coordinates | 497584..498855 (+) | Length | 424 a.a. |
Antitoxin (Protein)
| Gene name | HipB1 | Uniprot ID | A0A0H3C5I2 |
| Locus tag | CCNA_00481 | Protein ID | YP_002515854.3 |
| Coordinates | 497352..497597 (+) | Length | 82 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CCNA_00476 | 493326..493709 | - | 384 | YP_002515849.1 | hypothetical protein | - |
| CCNA_00477 | 494090..494284 | - | 195 | YP_002515850.1 | excisionase | - |
| CCNA_00478 | 494406..495143 | - | 738 | YP_002515851.1 | hypothetical protein | - |
| CCNA_00479 | 495332..495655 | + | 324 | YP_002515852.1 | Helix-turn-helix DNA-binding protein | - |
| CCNA_00480 | 495724..497022 | - | 1299 | YP_002515853.2 | bacteriophage P4 integrase | - |
| CCNA_00481 | 497352..497597 | + | 246 | YP_002515854.3 | HipB transcriptional regulator | Antitoxin |
| CCNA_00482 | 497584..498855 | + | 1272 | YP_002515855.1 | toxin protein module HipA | Toxin |
| CCNA_00483 | 499379..500224 | - | 846 | YP_002515856.1 | Zn-dependent hydrolase, glyoxalase II family | - |
| CCNA_00484 | 500314..501015 | - | 702 | YP_002515857.2 | hypothetical protein | - |
| CCNA_00485 | 501114..502142 | - | 1029 | YP_002515858.1 | ribosomal large subunit pseudouridine synthase D | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 424 a.a. Molecular weight: 45484.22 Da Isoelectric Point: 7.3296
>T10104 YP_002515855.1 NC_011916:497584-498855 [Caulobacter vibrioides NA1000]
VAMTRVLTVWWGDGVVGSLALDETGDIRFAYDAAWLADEACPAVSVSLPKQPGAFSRRKTRSFFAGLLPDDLQRDNVARA
LGVSRQNDFGLLERLGGDVAGALTLWPEGEAPPKRQGGRTAEPLNDKRLLAILDELPRRPFLAGEDGVRLSLAGAQEKLP
VVLVDGAVALPALGQPSTHILKPANARWPAMTENEALAMRLAAAVGLPTAGVEPRRIGERTFLLVTRYDRVVSSDGAVRR
LHQEDFCQALGVSPEHKYAAEGGPIFPDCFNLVRNVCQPSAPAVLALLDAAIFNVIVGNADAHGKNYSLLHQAGAIVMAP
LYDLMCTAAYPEVHAKLAMKIAGRAQLEAFKADTWRDFGRDIGMGPAFVERRANAQARRVLDQVGPVADAIAACGFDREA
LRGLRDLIAERARAVLKLGQGER
VAMTRVLTVWWGDGVVGSLALDETGDIRFAYDAAWLADEACPAVSVSLPKQPGAFSRRKTRSFFAGLLPDDLQRDNVARA
LGVSRQNDFGLLERLGGDVAGALTLWPEGEAPPKRQGGRTAEPLNDKRLLAILDELPRRPFLAGEDGVRLSLAGAQEKLP
VVLVDGAVALPALGQPSTHILKPANARWPAMTENEALAMRLAAAVGLPTAGVEPRRIGERTFLLVTRYDRVVSSDGAVRR
LHQEDFCQALGVSPEHKYAAEGGPIFPDCFNLVRNVCQPSAPAVLALLDAAIFNVIVGNADAHGKNYSLLHQAGAIVMAP
LYDLMCTAAYPEVHAKLAMKIAGRAQLEAFKADTWRDFGRDIGMGPAFVERRANAQARRVLDQVGPVADAIAACGFDREA
LRGLRDLIAERARAVLKLGQGER
Download Length: 1272 bp
>T10104 NC_011916:497584-498855 [Caulobacter vibrioides NA1000]
GTGGCGATGACTAGAGTGCTGACGGTCTGGTGGGGTGACGGCGTTGTCGGTTCGCTCGCGCTCGACGAGACCGGCGACAT
CCGTTTTGCCTATGACGCTGCCTGGCTGGCCGATGAGGCCTGTCCGGCCGTGTCGGTGTCCTTGCCTAAACAACCTGGCG
CCTTCAGCCGCCGCAAGACGCGCTCGTTCTTCGCGGGCCTGCTGCCTGACGACTTGCAGCGGGACAATGTCGCTCGGGCC
CTCGGTGTTTCCAGGCAGAACGACTTTGGCCTGCTGGAGCGCCTGGGCGGCGACGTCGCCGGCGCGCTCACACTCTGGCC
GGAGGGGGAGGCGCCTCCAAAGCGCCAAGGGGGGCGGACCGCTGAGCCGCTAAACGACAAGCGCCTGCTCGCCATTCTCG
ATGAGCTGCCCCGCCGCCCCTTCCTGGCCGGCGAAGACGGCGTTCGCTTGTCCCTCGCGGGCGCCCAGGAGAAGCTGCCA
GTCGTTCTGGTCGACGGAGCTGTTGCTCTGCCCGCGCTCGGCCAGCCCAGCACCCATATCCTCAAGCCAGCGAACGCACG
GTGGCCGGCCATGACGGAGAACGAGGCCCTTGCCATGCGGCTGGCGGCCGCTGTTGGGCTGCCGACGGCGGGTGTAGAGC
CGCGCAGGATCGGTGAGCGCACCTTTCTGCTGGTCACCCGCTATGACCGGGTCGTCAGCTCAGACGGTGCGGTGCGTCGC
CTGCACCAAGAAGACTTCTGCCAAGCGCTTGGCGTCTCGCCGGAACACAAATACGCGGCCGAGGGCGGGCCGATCTTTCC
GGACTGCTTCAACCTCGTCCGCAACGTCTGTCAGCCGTCCGCGCCGGCGGTGCTGGCGCTGCTCGATGCGGCGATTTTCA
ACGTCATCGTCGGCAACGCCGACGCTCACGGCAAGAACTACAGCCTGCTCCACCAAGCCGGCGCAATCGTCATGGCGCCG
CTCTATGACCTGATGTGCACGGCCGCCTATCCCGAGGTCCACGCCAAGCTGGCCATGAAGATCGCTGGGCGCGCCCAACT
GGAGGCGTTCAAGGCCGACACCTGGCGTGACTTCGGGCGCGATATCGGCATGGGTCCGGCGTTCGTTGAGCGTCGCGCGA
ACGCCCAAGCGCGACGGGTGCTCGACCAGGTGGGGCCCGTCGCCGACGCCATCGCCGCTTGCGGCTTTGACCGCGAGGCG
CTGCGCGGCTTGCGCGACCTGATCGCGGAGCGGGCGAGAGCCGTCCTGAAACTGGGTCAGGGCGAGCGCTAG
GTGGCGATGACTAGAGTGCTGACGGTCTGGTGGGGTGACGGCGTTGTCGGTTCGCTCGCGCTCGACGAGACCGGCGACAT
CCGTTTTGCCTATGACGCTGCCTGGCTGGCCGATGAGGCCTGTCCGGCCGTGTCGGTGTCCTTGCCTAAACAACCTGGCG
CCTTCAGCCGCCGCAAGACGCGCTCGTTCTTCGCGGGCCTGCTGCCTGACGACTTGCAGCGGGACAATGTCGCTCGGGCC
CTCGGTGTTTCCAGGCAGAACGACTTTGGCCTGCTGGAGCGCCTGGGCGGCGACGTCGCCGGCGCGCTCACACTCTGGCC
GGAGGGGGAGGCGCCTCCAAAGCGCCAAGGGGGGCGGACCGCTGAGCCGCTAAACGACAAGCGCCTGCTCGCCATTCTCG
ATGAGCTGCCCCGCCGCCCCTTCCTGGCCGGCGAAGACGGCGTTCGCTTGTCCCTCGCGGGCGCCCAGGAGAAGCTGCCA
GTCGTTCTGGTCGACGGAGCTGTTGCTCTGCCCGCGCTCGGCCAGCCCAGCACCCATATCCTCAAGCCAGCGAACGCACG
GTGGCCGGCCATGACGGAGAACGAGGCCCTTGCCATGCGGCTGGCGGCCGCTGTTGGGCTGCCGACGGCGGGTGTAGAGC
CGCGCAGGATCGGTGAGCGCACCTTTCTGCTGGTCACCCGCTATGACCGGGTCGTCAGCTCAGACGGTGCGGTGCGTCGC
CTGCACCAAGAAGACTTCTGCCAAGCGCTTGGCGTCTCGCCGGAACACAAATACGCGGCCGAGGGCGGGCCGATCTTTCC
GGACTGCTTCAACCTCGTCCGCAACGTCTGTCAGCCGTCCGCGCCGGCGGTGCTGGCGCTGCTCGATGCGGCGATTTTCA
ACGTCATCGTCGGCAACGCCGACGCTCACGGCAAGAACTACAGCCTGCTCCACCAAGCCGGCGCAATCGTCATGGCGCCG
CTCTATGACCTGATGTGCACGGCCGCCTATCCCGAGGTCCACGCCAAGCTGGCCATGAAGATCGCTGGGCGCGCCCAACT
GGAGGCGTTCAAGGCCGACACCTGGCGTGACTTCGGGCGCGATATCGGCATGGGTCCGGCGTTCGTTGAGCGTCGCGCGA
ACGCCCAAGCGCGACGGGTGCTCGACCAGGTGGGGCCCGTCGCCGACGCCATCGCCGCTTGCGGCTTTGACCGCGAGGCG
CTGCGCGGCTTGCGCGACCTGATCGCGGAGCGGGCGAGAGCCGTCCTGAAACTGGGTCAGGGCGAGCGCTAG
Antitoxin
Download Length: 82 a.a. Molecular weight: 8434.82 Da Isoelectric Point: 10.6826
>AT10104 YP_002515854.3 NC_011916:497352-497597 [Caulobacter vibrioides NA1000]
MTLLRQGLDAAALGAQVRRARRAMGLRQDELAAAAGVGLRFLVELERGKPTVQLGKVLDVLAAAGLDLQLVSRAPVSGGD
D
MTLLRQGLDAAALGAQVRRARRAMGLRQDELAAAAGVGLRFLVELERGKPTVQLGKVLDVLAAAGLDLQLVSRAPVSGGD
D
Download Length: 246 bp
>AT10104 NC_011916:497352-497597 [Caulobacter vibrioides NA1000]
ATGACCCTCCTCCGGCAAGGCCTGGACGCCGCAGCGCTAGGGGCGCAGGTGCGTCGGGCCCGTAGGGCTATGGGGCTGCG
ACAGGACGAGCTGGCCGCTGCTGCGGGCGTTGGCCTCCGGTTCCTCGTCGAGCTCGAAAGAGGCAAGCCGACGGTTCAGT
TGGGCAAGGTCTTGGATGTCCTGGCCGCCGCTGGCCTGGATCTGCAACTGGTCAGCCGTGCTCCGGTGAGCGGTGGCGAT
GACTAG
ATGACCCTCCTCCGGCAAGGCCTGGACGCCGCAGCGCTAGGGGCGCAGGTGCGTCGGGCCCGTAGGGCTATGGGGCTGCG
ACAGGACGAGCTGGCCGCTGCTGCGGGCGTTGGCCTCCGGTTCCTCGTCGAGCTCGAAAGAGGCAAGCCGACGGTTCAGT
TGGGCAAGGTCTTGGATGTCCTGGCCGCCGCTGGCCTGGATCTGCAACTGGTCAGCCGTGCTCCGGTGAGCGGTGGCGAT
GACTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10075 | Caulobacter crescentus NA1000 |
35.351 |
97.636 |
0.345 |
| T6108 | Burkholderia pseudomallei K96243 |
32.039 |
97.4 |
0.312 |
| T10106 | Xenorhabdus nematophila ATCC 19061 |
31.477 |
97.636 |
0.307 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10075 | Caulobacter crescentus NA1000 |
62.903 |
76.543 |
0.481 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3C5Z1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3C5I2 |
References
(1) Charlie Y Huang et al. (2020) hipBA toxin-antitoxin systems mediate persistence in Caulobacter crescentus. Scientific Reports 10(1):2865. [PubMed:32071324]