Detailed information of TA system TA210401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2994401..2996586
• Replicon: chromosome
• Accession: NZ_CP079926
• Organism: Caulobacter vibrioides strain CB S2B lysogen

Toxin (Protein)

• Gene name: HipA2
• Uniprot ID: Q9A4U2
• Locus tag: KZH45_RS14530
• Protein ID: WP_010920579.1
• Coordinates: 2994401..2995735 (-)
• Length: 445 a.a.

Antitoxin (Protein)

• Gene name: HipB2
• Uniprot ID: -
• Locus tag: KZH45_RS14535
• Protein ID: WP_010920580.1
• Coordinates: 2995732..2996586 (-)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZH45_RS14505 (KZH45_14500)	2990460..2990831	-	372	WP_010920575.1	hypothetical protein	-
KZH45_RS14510 (KZH45_14505)	2990902..2991105	-	204	WP_024265842.1	hypothetical protein	-
KZH45_RS14515 (KZH45_14510)	2991784..2991897	+	114	WP_012640570.1	hypothetical protein	-
KZH45_RS14520 (KZH45_14515)	2992123..2992278	-	156	WP_012640571.1	hypothetical protein	-
KZH45_RS14525 (KZH45_14520)	2992288..2994177	+	1890	WP_010920578.1	ubiquitin-activating E1 FCCH domain-containing protein	-
KZH45_RS14530 (KZH45_14525)	2994401..2995735	-	1335	WP_010920579.1	type II toxin-antitoxin system HipA family toxin	Toxin
KZH45_RS14535 (KZH45_14530)	2995732..2996586	-	855	WP_010920580.1	helix-turn-helix transcriptional regulator	Antitoxin
KZH45_RS14540 (KZH45_14535)	2996593..2996715	-	123	WP_233279858.1	hypothetical protein	-
KZH45_RS20185	2996664..2996834	+	171	WP_233279859.1	hypothetical protein	-
KZH45_RS14545 (KZH45_14540)	2996835..2996960	-	126	WP_012640572.1	hypothetical protein	-
KZH45_RS14550 (KZH45_14545)	2997145..2997498	+	354	WP_010920582.1	hypothetical protein	-
KZH45_RS14555 (KZH45_14550)	2997524..2997901	+	378	WP_233279860.1	DDE-type integrase/transposase/recombinase	-
KZH45_RS14560 (KZH45_14555)	2998032..2999054	+	1023	WP_012640573.1	IS110-like element ISCc4 family transposase	-
KZH45_RS14565 (KZH45_14560)	2999179..3000365	+	1187	WP_096053990.1	IS3-like element IS511 family transposase	-
KZH45_RS14570 (KZH45_14565)	3000337..3001521	+	1185	WP_010920585.1	S8 family serine peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002696 Caulobacter crescentus CB15 Genomic island TA020378 2941644..2956542 0 View NC_011916 Caulobacter crescentus NA1000 Genomic island TA010075 2967624..2982522 0	inside	Genomic island	-	-	2984547..2999445	14898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-387)

Antitoxin

• Cro/C1-type helix-turn-helix domain (225-279)

Sequences

Toxin

Protein

• Length: 445 a.a.
• Molecular weight: 48396.41 Da
• Isoelectric Point: 5.2025

>T210401 WP_010920579.1 NZ_CP079926:c2995735-2994401 [Caulobacter vibrioides]
MSGPAGLIVRMDGFNLPAGYLASDEARAISFAYDDRYIAAGGPPLSLSMPLEQVSFGDVTARAFFDNLLPENDQMQRVMD
REGLARDDIVGLLSHLGADCSGAISCLPIGADPIKVPGVLSEDYELLAPGAIAEIARSLAERQRLPDTITDPSPVAGVQR
KIALTHTPQGFAKPRPGRKVPTTHILKVPETRLRRDARLEAAAARLASALGLDVSIPEAIVIDGVDALLITRFDRVVRDG
VVYRLHQEDFAQAMGLPATLKYQRNGAPGRQFDAQAIARVLDQTEAPALSRTAFLSATIFNLLIGNTDNHAKNHGLLYRQ
GRAPILAPLYDLLPSRMNLDFNDQLSFNIGAADHPDAITFDDMMAFFEVFGMRRAAAARFIENVIKPMIEALERATLGLA
GERLKDMDDLIGRETEQLVEVLGLDVAVRERDYYPKVRHALAPS

Nucleotide

• Length: 1335 bp

>T210401 NZ_CP104516:924554-924661 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGGACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 28530.71 Da
• Isoelectric Point: 10.0342

>AT210401 WP_010920580.1 NZ_CP079926:c2996586-2995732 [Caulobacter vibrioides]
MLLSGSENVSDDNKGKLGIGREASVPSAADLLKRAMGGSLARDALKIAGVSGATSELLASIGRIGVASDALKMAGITSVA
SDALKIAGVGAASDALRVVSAGGLKPEFLASIGKISAASDALKLASVTSAADILKNADVGRLNAIAPPSISLASAAGGAR
QGGELSPLSDILDATRHLTNERYAVTAPSPSAADSLKQTDGGAQYNLPIARMQVPQPIKSPRALGQLVRQARERMGLTQA
AFADAAGVGRRFVSELENGKPTLELGKVMAVCAAAGLDLMAAPR

Nucleotide

• Length: 855 bp

>AT210401 NZ_CP104516:c924504-924441 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: Q9A4U2