Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Xre-RelE
Location 1071285..1071933 Replicon chromosome
Accession NC_003028
Organism Streptococcus pneumoniae TIGR4

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag SP_1143 Protein ID 15901009
Coordinates 1071285..1071650 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag SP_1144 Protein ID 15901010
Coordinates 1071640..1071933 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SP_RS05615 (SP_1134) 1066524..1067141 + 618 WP_000424676.1 Rha family transcriptional regulator -
SP_RS05620 (SP_1135) 1067176..1067628 + 453 WP_000141102.1 hypothetical protein -
SP_RS05625 (SP_1136) 1067625..1068071 + 447 WP_000171242.1 DnaD domain protein -
SP_RS05630 (SP_1137) 1068083..1068967 + 885 WP_000838905.1 ATP-binding protein -
SP_RS05635 (SP_1139) 1069216..1069866 + 651 WP_000138311.1 hypothetical protein -
SP_RS05640 (SP_1140) 1069913..1070341 + 429 WP_000918210.1 hypothetical protein -
SP_RS05645 (SP_1141) 1070349..1070660 + 312 WP_001113833.1 hypothetical protein -
SP_RS05650 (SP_1142) 1070678..1071088 + 411 WP_000162610.1 hypothetical protein -
SP_RS05655 (SP_1143) 1071285..1071650 + 366 WP_000551587.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
SP_RS05660 (SP_1144) 1071640..1071933 + 294 WP_000794247.1 helix-turn-helix transcriptional regulator Antitoxin
SP_RS05665 (SP_1145) 1071951..1073141 + 1191 WP_000123149.1 hypothetical protein -
SP_RS13035 (SP_1146) 1073144..1073320 + 177 WP_000346914.1 hypothetical protein -
SP_RS12125 1073640..1074086 - 447 WP_061631657.1 tyrosine-type recombinase/integrase -
SP_RS12130 1073986..1074831 - 846 Protein_1067 IS630 family transposase -
SP_RS12795 1075002..1075157 + 156 Protein_1068 diacylglycerol kinase family lipid kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1063231..1083773 20542


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(35-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-88)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 14514.67 Da        Isoelectric Point: 9.8086

>T325 15901009 NC_003028:1071285-1071650 [Streptococcus pneumoniae TIGR4]
VHNIYFYKDKNGNEPVFDYMRELTSKKGKDSRIKLNKINDYIELLSQHGTRAGEPYIKHLDAEIWELRPLRDRILFVAWM
DGSFVLLHHFMKRTQKTPKREIEQAKRELADLKERGLDNEK

Download         Length: 366 bp

>T325 NC_003028:1071285-1071650 [Streptococcus pneumoniae TIGR4]
GTGCATAATATCTATTTTTACAAGGATAAAAACGGCAATGAGCCTGTTTTTGATTATATGCGAGAGCTTACCAGTAAAAA
AGGGAAAGATAGCAGAATTAAGCTTAATAAAATTAATGATTATATTGAGTTGTTAAGCCAACATGGAACACGCGCAGGCG
AACCATATATTAAGCATTTAGATGCTGAAATTTGGGAGCTGAGACCACTTAGAGATAGAATTTTATTTGTTGCTTGGATG
GATGGTAGTTTTGTTTTACTGCATCATTTTATGAAAAGGACACAGAAAACACCCAAAAGAGAGATTGAGCAAGCTAAACG
TGAACTTGCAGATTTAAAAGAAAGAGGTTTAGACAATGAAAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10751.45 Da        Isoelectric Point: 5.1991

>AT325 15901010 NC_003028:1071640-1071933 [Streptococcus pneumoniae TIGR4]
MKNNAIGSNWKDVRAELFSKEEILESDMRVAIMSELIEARNEKGISQKKLEEMSGVSQPVIARMETGKTSPQLDTVLKVL
ASLGKTLAVVPLEHEQV

Download         Length: 294 bp

>AT325 NC_003028:1071640-1071933 [Streptococcus pneumoniae TIGR4]
ATGAAAAATAATGCTATTGGTAGTAACTGGAAAGATGTAAGAGCTGAATTATTCAGCAAAGAGGAAATTTTGGAAAGTGA
TATGCGCGTGGCTATCATGAGTGAGCTTATCGAGGCTAGAAATGAAAAGGGTATTAGTCAGAAGAAACTAGAGGAGATGA
GCGGTGTTAGTCAGCCAGTTATCGCTAGAATGGAAACAGGAAAGACAAGCCCACAACTTGATACAGTATTAAAAGTGTTG
GCAAGTCTTGGTAAGACCTTAGCTGTTGTACCATTAGAGCATGAGCAGGTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T1067 Haemophilus influenzae Rd KW20

63.333

100

0.639

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT1067 Haemophilus influenzae Rd KW20

57.143

93.814

0.536

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References

(1) Sung-Min Kang et al. (2021) Structure-based design of peptides that trigger Streptococcus pneumoniae cell death. The FEBS Journal 288(5):1546-1564. [PubMed:32770723] 3D_structure


3D_structure protein structure literature