1067

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) toxTA/Xre-RelE
Location 702730..703378 Replicon chromosome
Accession NC_000907
Organism Haemophilus influenzae Rd KW20

Toxin (Protein)


Gene name toxT Uniprot ID P44031
Locus tag HI0660 Protein ID 16272603
Coordinates 703019..703378 (-) Length 120 a.a.

Antitoxin (Protein)


Gene name toxA Uniprot ID P44030
Locus tag HI0659 Protein ID 16272602
Coordinates 702730..703026 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI_RS03495 (HI0654) 698164..698721 - 558 WP_005694489.1 DNA-3-methyladenine glycosylase I -
HI_RS03500 (HI0655) 698718..699536 - 819 WP_005694487.1 shikimate dehydrogenase -
HI_RS03505 (HI0656) 699540..700091 - 552 WP_005694486.1 Sua5/YciO/YrdC/YwlC family protein -
HI_RS03510 (HI0656.1) 700107..700643 - 537 WP_005694485.1 type I DNA topoisomerase -
HI_RS03515 (HI0658) 700653..702569 - 1917 WP_005694483.1 ABC transporter ATP-binding protein -
HI_RS03520 (HI0659) 702730..703026 - 297 WP_005650837.1 helix-turn-helix transcriptional regulator Antitoxin
HI_RS03525 (HI0660) 703019..703378 - 360 WP_005694481.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HI_RS03530 (HI0661) 703648..706644 - 2997 WP_164927859.1 TonB-dependent hemoglobin/transferrin/lactoferrin family receptor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(36-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(34-89)


Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 14321.67 Da        Isoelectric Point: 10.1408

>T1067 16272603 NC_000907:c703378-703019 [Haemophilus influenzae Rd KW20]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVKLLSELGTSVGKPYVKHLDGEIWELRPIRDRILFARLM
DGRFVLLHQFMKKTQKTPKREIQTAQQRLSELKERLKNE

Download         Length: 360 bp

>T1067 NC_000907:c703378-703019 [Haemophilus influenzae Rd KW20]
ATGTACGAGATTCTCTTTTACAGAGACCAAAATGACATTGAGCCAGTGAAAGAGTATCTGCTATCTCTGGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAAAGTTATTAAGCGAACTCGGGACGTCTGTTGGCA
AACCCTATGTGAAACATTTAGACGGTGAAATTTGGGAACTTCGTCCAATTAGGGATCGCATCTTGTTTGCAAGATTAATG
GATGGTCGCTTTGTCTTACTACATCAATTTATGAAGAAAACACAAAAAACGCCAAAACGAGAAATCCAAACAGCCCAACA
ACGCTTAAGTGAATTAAAAGAGAGATTGAAAAATGAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11092.94 Da        Isoelectric Point: 10.3139

>AT1067 16272602 NC_000907:c703026-702730 [Haemophilus influenzae Rd KW20]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Download         Length: 297 bp

>AT1067 NC_000907:c703026-702730 [Haemophilus influenzae Rd KW20]
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAGCAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAGCTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTTCCTCTAAGGGTAAAAAATGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T325 Streptococcus pneumoniae TIGR4

63.333

100

0.639

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT325 Streptococcus pneumoniae TIGR4

57.143

93.814

0.536

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB P44031

Antitoxin

Source ID Structure
AlphaFold DB P44030

References

(1) Hailey Findlay Black et al. (2020) A competence-regulated toxin-antitoxin system in Haemophilus influenzae. PloS One 15(1):e0217255. [PubMed:31931516] experimental


experimental experimental literature