Detailed information of regulator


Summary

Regulator ID REG0140 experimental
Regulator type protein
Regulatory pathway (from literatures) bspR-dependent signalling cascade
KEGG pathway (determined by BlastKOALA) -
Related T6SS -
Strain Burkholderia pseudomallei strain KHW
Replicon chromosome
Sequence Protein sequence (324 a.a.); Nucleotide sequence (972 bp)
Description bspR (bsa‐specific regulator) Deletion of bspR, bprP or bsaN reduced the expression of T6SS5 genes to less than 8% of wild‐type level.
Reference
External database links

Locus tag (Gene) bprp
Coordinates (Strand) 175..1146 (+)
NCBI ID 298239796
GenBank HM447214
Uniprot ID D7SFB6_BURPE
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
tssA expression ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Trans_reg_C PF00486.30 2.4e-05 45..115
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-221 MGRFASPSNDFSNSEKRHILSKYQIDGSVVFDSQAMTLSRGDIVTTLTANETELLCILMQGTASKQAVIEQIWESKGLFVTEGSYHQLVRALRVKLDEQGIPPTQVKTLPRLGLRFLGFAELLDASPPSPDAHAASASPASSEPEPAAAPAGAQAALAEAIAPSVPIAPRASSAPDTANTVTAPIAAAAPPPSAAPSAPPPGAASAPLPATRAAHARSPRR
Transmembrane helix 222-244 RAAYLAIYVTLAAWASVLTWQTF
Outside 245-323 RHRDNTFRFRFTRTVDGIHYFSDGRMNQPNLLKAIDVKPLKGSFVYEIELGSNDWLAVCPESIYETPELCQAYFFEKNN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9903 - -


  Protein sequence of regulator: 324 a.a.    .

>REG0140 HM447214:175-1146 [Burkholderia pseudomallei]
MGRFASPSNDFSNSEKRHILSKYQIDGSVVFDSQAMTLSRGDIVTTLTANETELLCILMQGTASKQAVIEQIWESKGLFV
TEGSYHQLVRALRVKLDEQGIPPTQVKTLPRLGLRFLGFAELLDASPPSPDAHAASASPASSEPEPAAAPAGAQAALAEA
IAPSVPIAPRASSAPDTANTVTAPIAAAAPPPSAAPSAPPPGAASAPLPATRAAHARSPRRRAAYLAIYVTLAAWASVLT
WQTFRHRDNTFRFRFTRTVDGIHYFSDGRMNQPNLLKAIDVKPLKGSFVYEIELGSNDWLAVCPESIYETPELCQAYFFE
KNN*

  Nucleotide sequence of regulator: 972 bp    .

>REG0140 HM447214:175-1146 [Burkholderia pseudomallei]
ATGGGGCGATTCGCATCGCCTTCGAATGATTTTTCAAACAGTGAGAAACGCCATATTTTGAGCAAGTACCAGATCGACGG
CAGTGTCGTATTCGATAGTCAAGCGATGACGCTTTCTCGCGGCGACATCGTCACCACGCTTACCGCCAACGAAACCGAGC
TGCTTTGCATCTTGATGCAGGGCACGGCCAGCAAGCAGGCCGTGATCGAGCAAATCTGGGAGAGCAAGGGGCTCTTCGTC
ACGGAAGGCAGTTATCACCAGCTCGTGCGCGCGCTGCGCGTGAAGCTCGACGAGCAAGGCATCCCGCCCACGCAAGTGAA
GACGCTGCCGCGGCTCGGCCTGCGTTTTCTCGGCTTCGCGGAGCTGCTCGACGCATCGCCGCCTTCGCCGGATGCGCACG
CCGCGTCCGCCTCGCCCGCTTCGTCCGAGCCCGAACCCGCCGCCGCGCCGGCCGGCGCACAAGCGGCGCTCGCCGAAGCG
ATCGCGCCGAGCGTGCCCATCGCACCGCGCGCATCGAGCGCACCGGATACGGCGAATACAGTCACTGCGCCGATCGCGGC
CGCCGCACCGCCGCCATCCGCCGCGCCATCCGCGCCGCCGCCCGGCGCGGCAAGCGCGCCGTTGCCCGCCACGCGCGCCG
CGCATGCGCGATCGCCGCGCCGCCGAGCAGCCTATCTCGCGATCTACGTGACGCTCGCGGCGTGGGCGAGCGTGCTCACC
TGGCAAACGTTCAGGCATCGCGACAACACCTTCCGGTTCCGCTTCACGCGGACCGTCGACGGCATCCACTACTTCTCCGA
CGGCCGCATGAACCAGCCCAACCTGCTGAAAGCCATCGACGTCAAACCGCTCAAGGGCAGCTTCGTCTACGAGATCGAAC
TGGGATCGAACGACTGGCTCGCGGTATGTCCGGAATCCATCTACGAAACACCTGAACTGTGCCAAGCCTATTTCTTCGAA
AAGAACAACTGA