Detailed information of regulator
Summary
| Regulator ID | REG0125  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS01278 (Type i1) |
| Strain | Aliivibrio fischeri strain FQ-A001 |
| Replicon | chromosome |
| Sequence | Protein sequence (167 a.a.); Nucleotide sequence (501 bp) |
| Description | Relative abundance of ES114 following coincubation with the ΔvasH mutant was comparable to the Hcp− control, suggesting a strong dependence of T6SS-dependent killing on VasH in FQ-A001 and coincubation of ES114 and the ΔvasH mutant harboring a Ptrc-vasH in the presence of IPTG resulted in low CFP fluorescence. |
| Reference |
External database links
| Locus tag (Gene) | VFFQA001_15620 (VasH) |
| Coordinates (Strand) | 359608..360108 (+) |
| NCBI ID | 1027725198 |
| RefSeq | NZ_SJSX01000004 |
| Uniprot ID | UPI0007C56580 |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Unknown | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-166 | MKPYLLLLPAALIVTGCSNQTSVPDPLAPYKNVHIIGNKKQSIGSFNYLSKDVDGHTDKQVIVYFNEIPFTSTFNLCADKGVYIANCKSTVSIFPSKNKGVIFNYDLNFYNGSYPARVGSMNILLPSTTTRKMDNLTQYTSAKFSEEQFLESETLEEATFILMQSS |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9992 | pos: 16-17. VTG-CS. | MKPYLLLLPAALIVTG |
Protein sequence of regulator: 167 a.a. .
>REG0125 NZ_SJSX01000004:359608-360108 [Aliivibrio fischeri]
MKPYLLLLPAALIVTGCSNQTSVPDPLAPYKNVHIIGNKKQSIGSFNYLSKDVDGHTDKQVIVYFNEIPFTSTFNLCADK
GVYIANCKSTVSIFPSKNKGVIFNYDLNFYNGSYPARVGSMNILLPSTTTRKMDNLTQYTSAKFSEEQFLESETLEEATF
ILMQSS*
MKPYLLLLPAALIVTGCSNQTSVPDPLAPYKNVHIIGNKKQSIGSFNYLSKDVDGHTDKQVIVYFNEIPFTSTFNLCADK
GVYIANCKSTVSIFPSKNKGVIFNYDLNFYNGSYPARVGSMNILLPSTTTRKMDNLTQYTSAKFSEEQFLESETLEEATF
ILMQSS*
Nucleotide sequence of regulator: 501 bp .
>REG0125 NZ_SJSX01000004:359608-360108 [Aliivibrio fischeri]
ATGAAACCATACTTACTATTATTACCAGCAGCGCTTATTGTTACTGGTTGTTCCAATCAAACATCAGTTCCTGATCCATT
GGCTCCTTATAAAAATGTGCACATTATAGGAAACAAAAAACAAAGTATTGGTTCATTTAACTACTTATCTAAAGATGTCG
ATGGGCATACAGATAAACAAGTGATTGTTTATTTTAATGAGATCCCTTTCACTAGCACGTTCAATTTATGTGCAGATAAA
GGTGTTTATATTGCTAACTGCAAAAGTACAGTAAGTATATTCCCAAGTAAAAACAAAGGTGTTATTTTCAACTACGATTT
GAATTTTTATAATGGTAGCTACCCCGCAAGAGTTGGCAGTATGAATATACTACTACCAAGTACAACCACTCGTAAGATGG
ATAACCTAACCCAATACACGTCGGCAAAATTCAGTGAAGAGCAATTCTTAGAAAGTGAAACACTAGAAGAAGCTACATTT
ATTTTGATGCAGTCATCTTAA
ATGAAACCATACTTACTATTATTACCAGCAGCGCTTATTGTTACTGGTTGTTCCAATCAAACATCAGTTCCTGATCCATT
GGCTCCTTATAAAAATGTGCACATTATAGGAAACAAAAAACAAAGTATTGGTTCATTTAACTACTTATCTAAAGATGTCG
ATGGGCATACAGATAAACAAGTGATTGTTTATTTTAATGAGATCCCTTTCACTAGCACGTTCAATTTATGTGCAGATAAA
GGTGTTTATATTGCTAACTGCAAAAGTACAGTAAGTATATTCCCAAGTAAAAACAAAGGTGTTATTTTCAACTACGATTT
GAATTTTTATAATGGTAGCTACCCCGCAAGAGTTGGCAGTATGAATATACTACTACCAAGTACAACCACTCGTAAGATGG
ATAACCTAACCCAATACACGTCGGCAAAATTCAGTGAAGAGCAATTCTTAGAAAGTGAAACACTAGAAGAAGCTACATTT
ATTTTGATGCAGTCATCTTAA