Detailed information of regulator
Summary
| Regulator ID | REG0094  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00040 (Type i1) |
| Strain | Pectobacterium atrosepticum SCRI1043 |
| Replicon | chromosome |
| Sequence | Protein sequence (518 a.a.); Nucleotide sequence (1554 bp) |
| Description | The reporter fusion assays results that the bEBPs bind to the T6SS gene cluster promoters to activate their transcription and EMSA assays results that bEBPs specifically bind the promoter region of cognate T6SS and hcp orphon gene cluster. |
| Reference |
External database links
| Locus tag (Gene) | ECA3435 (bEBP/VasH) |
| Coordinates (Strand) | 3850745..3852298 (-) |
| NCBI ID | 499407476 |
| RefSeq | NC_004547 |
| Uniprot ID | Q6D1L1_PECAS |
| KEGG ID | eca:ECA3435 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| T6SS gene cluster promoter (PECA3445) | ↓ | catgtgtctataccaacgtaagggcactcgggcccggagaagtaaagtcgattcctaccaaaagcccgccataatccataagcacggcagcgactcggcaaacaaatatccccgtcatccttcaagttgcatgtgcgttggctgcgttcagtcatccgaatcacttaccagagtaagctcatcggaattccttatcttgccgccttcctgaaactcgaattatttagggtatagcgtatttgtttttcgaccagcgagatgaaataaatttaaatcgaattaaaaaaaccataataaagccgtaaatttacggtttatatcgatgtaaaagagtcacaccaagaaggaagttaaatacatttatattttgagtttagatcatacacacaacacaaacgcatggtcaatgcttatatagcaaagcaagtaaacagaacaactaaaacaaaaacacaataaaaacaaaagattaaattaaacttaagtaaaaatatccataaaaaaacagaataagtcattgagtttcaattgactgaaaaagaatatatttcgtgccttttaatttcgttaacaacgaaatacttaacccgctaacaaattaagg |
| Hcp gene cluster promoter (PECA4275) | ↓ | accggcaagtatcagcaataagtgtttctctcggtcacgattatctatcgccgctaaccggcgcagccttaacgagtgtaggacaacacgccagcgcatgcccaataggcgtatcccgttaagatcctgaccgagaatgacatacgcattatgccagttaatggcagcacttcactatgctgaaaaatgaatgatctcgtgtggcgggctccatagggaaaatcgcccatacttaatggcctatcaaaatcatcgagccgcactgacaaaccacttacgtaaaatcatgccaacgctagcttttacttttgttacccgaatgacgtcttttgctatgcaagatatgcatagcaaatataagtaattactcacttacatttttataaacgcgagcaaattatcgccagatatagctttttactatcgataaccaccgtttcgattggttattcatttcaataatttagtactaactcaaactttcagaataactgcgcattctgcgcaacttcttgctcactttctggtggaaatcactttttgcgcaatggatgccgttttttcgcgaggtctgcgcaattttttgctcaaattttgcttagggggaattttagttaagatatttatcattaaaaacaatcagatatgaattggcatacgaattgctatacagacggtgaagttactaaacccgttcaacaacaaggagcaacactatgccaactcc |
| Hcp gene cluster promoter (PECA2866) | ↓ | tgatattgataaatttatgaggatatcctccccattcatcgtcaataccttctaaatagacggacatctcacagttctaactcaaactttcagcataaccgcgcattctgcgcaacttcttgctcactttctggtggaaatcactttttgcgcaatggatgccgttttttcgcgaggtctgcgcaatttcttgctcaaatttgacttaggggaaatttgacttaagtaaattaacagcaaaaacaataacataaaatctggcatgcgaattgctatgcagagcgtgaagttactcatcacgttcaacaacaaggagcaagact |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Sigma54_activat | PF00158.28 | 1.4e-66 | 198..361 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-517 | MQHALKLALALTRQHDETSLCDWLLETMQAAWQPQGMLLGMVDVSGRQLICQGRVHETPVALSLGVDDFSHPLAYALHKNQARTWDSLYGGARIEHREFRQMLVSVGTSCGLHALPLLSDSGKPLAVLALLDTPTRLQTLHQRGECERLAQVFCRQLMLLRELVHTRREQVALRDSLRQIKDEGQSRREQKKRVEATLIGRSVVIKELHQQIYQAAKHRLSVLIQGETGSGKDVVARLLHECSERVDKPFIAINCAAIPENLIESELFGYQKGAFSGAQSNKIGLVEQANGGTLFLDEVGDMPQSMQAKLLRVLETHSFRPLGAEKEVHSDFRLIAATHQPLEQQVSDGVFRQDLYHRLCQCLLLVAPLRERPDDIRLLCEHFIGQFADKPFSDERQKPIGPLSRAFLRQLVGYDFPGNVRELRNLLEVACAHTPSGEALSLTSLPPELRDRLTSSTAEEQDCYQHIHDLRIATQRYEAAVIEARLRQFQGNRLLVADSLNIPKRTLDHKCQKLEVN |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9878 | - | - |
Protein sequence of regulator: 518 a.a. .
>REG0094 NC_004547:c3852298-3850745 [Pectobacterium atrosepticum SCRI1043]
MQHALKLALALTRQHDETSLCDWLLETMQAAWQPQGMLLGMVDVSGRQLICQGRVHETPVALSLGVDDFSHPLAYALHKN
QARTWDSLYGGARIEHREFRQMLVSVGTSCGLHALPLLSDSGKPLAVLALLDTPTRLQTLHQRGECERLAQVFCRQLMLL
RELVHTRREQVALRDSLRQIKDEGQSRREQKKRVEATLIGRSVVIKELHQQIYQAAKHRLSVLIQGETGSGKDVVARLLH
ECSERVDKPFIAINCAAIPENLIESELFGYQKGAFSGAQSNKIGLVEQANGGTLFLDEVGDMPQSMQAKLLRVLETHSFR
PLGAEKEVHSDFRLIAATHQPLEQQVSDGVFRQDLYHRLCQCLLLVAPLRERPDDIRLLCEHFIGQFADKPFSDERQKPI
GPLSRAFLRQLVGYDFPGNVRELRNLLEVACAHTPSGEALSLTSLPPELRDRLTSSTAEEQDCYQHIHDLRIATQRYEAA
VIEARLRQFQGNRLLVADSLNIPKRTLDHKCQKLEVN*
MQHALKLALALTRQHDETSLCDWLLETMQAAWQPQGMLLGMVDVSGRQLICQGRVHETPVALSLGVDDFSHPLAYALHKN
QARTWDSLYGGARIEHREFRQMLVSVGTSCGLHALPLLSDSGKPLAVLALLDTPTRLQTLHQRGECERLAQVFCRQLMLL
RELVHTRREQVALRDSLRQIKDEGQSRREQKKRVEATLIGRSVVIKELHQQIYQAAKHRLSVLIQGETGSGKDVVARLLH
ECSERVDKPFIAINCAAIPENLIESELFGYQKGAFSGAQSNKIGLVEQANGGTLFLDEVGDMPQSMQAKLLRVLETHSFR
PLGAEKEVHSDFRLIAATHQPLEQQVSDGVFRQDLYHRLCQCLLLVAPLRERPDDIRLLCEHFIGQFADKPFSDERQKPI
GPLSRAFLRQLVGYDFPGNVRELRNLLEVACAHTPSGEALSLTSLPPELRDRLTSSTAEEQDCYQHIHDLRIATQRYEAA
VIEARLRQFQGNRLLVADSLNIPKRTLDHKCQKLEVN*
Nucleotide sequence of regulator: 1554 bp .
>REG0094 NC_004547:c3852298-3850745 [Pectobacterium atrosepticum SCRI1043]
ATGCAACATGCCCTCAAACTGGCGCTAGCACTCACCCGACAGCATGATGAAACTAGTCTGTGCGACTGGCTGCTGGAGAC
GATGCAGGCCGCCTGGCAGCCGCAAGGGATGCTGCTGGGGATGGTGGATGTCAGCGGCAGACAGCTGATCTGTCAGGGGC
GGGTGCATGAGACGCCGGTGGCGCTGAGTCTGGGGGTGGATGACTTTAGCCATCCGCTGGCTTATGCGCTGCATAAGAAT
CAGGCGCGTACCTGGGATTCTCTTTATGGCGGTGCTCGTATTGAGCACCGTGAATTTCGGCAGATGCTGGTCTCTGTCGG
GACGAGTTGCGGGCTTCACGCGTTGCCGCTGTTGTCGGACAGCGGTAAGCCGCTGGCGGTATTAGCGCTGTTGGATACGC
CGACGCGCCTGCAAACGCTGCATCAGCGAGGCGAATGCGAGCGGCTGGCTCAGGTATTTTGCCGCCAGCTTATGCTGCTG
CGTGAGCTGGTACACACCCGACGGGAACAGGTCGCCTTGAGAGATTCTCTCCGCCAGATTAAGGATGAAGGGCAGAGTCG
TCGCGAGCAGAAAAAGCGGGTGGAAGCCACCCTGATTGGCCGATCTGTCGTGATTAAAGAGCTGCATCAGCAGATTTATC
AGGCGGCAAAACACCGTCTTTCAGTGCTGATTCAGGGCGAAACCGGTTCAGGGAAAGATGTGGTGGCTCGCTTGCTTCAT
GAGTGTTCCGAACGCGTCGACAAACCCTTTATTGCTATCAACTGTGCCGCAATCCCCGAAAATCTGATTGAAAGCGAACT
GTTTGGCTATCAGAAAGGGGCATTCTCTGGCGCACAAAGTAACAAAATTGGTCTGGTTGAGCAGGCTAACGGCGGCACGC
TGTTTTTGGATGAAGTCGGCGATATGCCGCAGTCCATGCAAGCCAAGCTGTTAAGGGTATTGGAGACACATAGCTTCCGC
CCTCTGGGCGCGGAGAAAGAAGTTCATTCAGATTTCCGTCTGATTGCCGCGACGCACCAGCCGCTGGAACAGCAGGTGTC
TGACGGCGTGTTCCGGCAGGATCTCTATCACCGCCTCTGCCAGTGCCTGTTGCTGGTCGCGCCGCTGCGTGAGCGGCCAG
ATGATATTCGTCTGCTGTGCGAGCACTTCATCGGTCAATTTGCAGATAAACCATTTTCGGATGAACGACAAAAGCCCATA
GGGCCATTGAGTCGCGCTTTCCTGCGCCAGCTCGTGGGGTACGACTTTCCCGGCAATGTGCGGGAACTACGTAACCTGCT
GGAAGTGGCGTGTGCACATACGCCGAGCGGTGAAGCGCTGTCCTTGACATCGCTGCCGCCCGAACTGCGTGACCGGCTGA
CGAGCAGCACGGCTGAGGAACAGGATTGTTATCAGCATATCCACGATTTGCGTATAGCCACACAGCGGTATGAAGCGGCT
GTGATTGAGGCACGCCTGCGCCAGTTTCAGGGCAACCGCCTGCTGGTAGCCGATAGCCTCAATATCCCTAAACGCACTCT
TGACCACAAATGCCAGAAGCTGGAGGTGAATTGA
ATGCAACATGCCCTCAAACTGGCGCTAGCACTCACCCGACAGCATGATGAAACTAGTCTGTGCGACTGGCTGCTGGAGAC
GATGCAGGCCGCCTGGCAGCCGCAAGGGATGCTGCTGGGGATGGTGGATGTCAGCGGCAGACAGCTGATCTGTCAGGGGC
GGGTGCATGAGACGCCGGTGGCGCTGAGTCTGGGGGTGGATGACTTTAGCCATCCGCTGGCTTATGCGCTGCATAAGAAT
CAGGCGCGTACCTGGGATTCTCTTTATGGCGGTGCTCGTATTGAGCACCGTGAATTTCGGCAGATGCTGGTCTCTGTCGG
GACGAGTTGCGGGCTTCACGCGTTGCCGCTGTTGTCGGACAGCGGTAAGCCGCTGGCGGTATTAGCGCTGTTGGATACGC
CGACGCGCCTGCAAACGCTGCATCAGCGAGGCGAATGCGAGCGGCTGGCTCAGGTATTTTGCCGCCAGCTTATGCTGCTG
CGTGAGCTGGTACACACCCGACGGGAACAGGTCGCCTTGAGAGATTCTCTCCGCCAGATTAAGGATGAAGGGCAGAGTCG
TCGCGAGCAGAAAAAGCGGGTGGAAGCCACCCTGATTGGCCGATCTGTCGTGATTAAAGAGCTGCATCAGCAGATTTATC
AGGCGGCAAAACACCGTCTTTCAGTGCTGATTCAGGGCGAAACCGGTTCAGGGAAAGATGTGGTGGCTCGCTTGCTTCAT
GAGTGTTCCGAACGCGTCGACAAACCCTTTATTGCTATCAACTGTGCCGCAATCCCCGAAAATCTGATTGAAAGCGAACT
GTTTGGCTATCAGAAAGGGGCATTCTCTGGCGCACAAAGTAACAAAATTGGTCTGGTTGAGCAGGCTAACGGCGGCACGC
TGTTTTTGGATGAAGTCGGCGATATGCCGCAGTCCATGCAAGCCAAGCTGTTAAGGGTATTGGAGACACATAGCTTCCGC
CCTCTGGGCGCGGAGAAAGAAGTTCATTCAGATTTCCGTCTGATTGCCGCGACGCACCAGCCGCTGGAACAGCAGGTGTC
TGACGGCGTGTTCCGGCAGGATCTCTATCACCGCCTCTGCCAGTGCCTGTTGCTGGTCGCGCCGCTGCGTGAGCGGCCAG
ATGATATTCGTCTGCTGTGCGAGCACTTCATCGGTCAATTTGCAGATAAACCATTTTCGGATGAACGACAAAAGCCCATA
GGGCCATTGAGTCGCGCTTTCCTGCGCCAGCTCGTGGGGTACGACTTTCCCGGCAATGTGCGGGAACTACGTAACCTGCT
GGAAGTGGCGTGTGCACATACGCCGAGCGGTGAAGCGCTGTCCTTGACATCGCTGCCGCCCGAACTGCGTGACCGGCTGA
CGAGCAGCACGGCTGAGGAACAGGATTGTTATCAGCATATCCACGATTTGCGTATAGCCACACAGCGGTATGAAGCGGCT
GTGATTGAGGCACGCCTGCGCCAGTTTCAGGGCAACCGCCTGCTGGTAGCCGATAGCCTCAATATCCCTAAACGCACTCT
TGACCACAAATGCCAGAAGCTGGAGGTGAATTGA