Detailed information of regulator


Summary

Regulator ID REG0094 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00040 experimental (Type i1)
Strain Pectobacterium atrosepticum SCRI1043
Replicon chromosome
Sequence Protein sequence (518 a.a.); Nucleotide sequence (1554 bp)
Description The reporter fusion assays results that the bEBPs bind to the T6SS gene cluster promoters to activate their transcription and EMSA assays results that bEBPs specifically bind the promoter region of cognate T6SS and hcp orphon gene cluster.
Reference

External database links

Locus tag (Gene) ECA3435 (bEBP/VasH)
Coordinates (Strand) 3850745..3852298 (-)
NCBI ID 499407476
RefSeq NC_004547
Uniprot ID Q6D1L1_PECAS
KEGG ID eca:ECA3435
KEGG pathway (determined by BlastKOALA) -
PDB ID -

  Target
Target Regulation (↓/↑) Target sequence
T6SS gene cluster promoter (PECA3445) catgtgtctataccaacgtaagggcactcgggcccggagaagtaaagtcgattcctaccaaaagcccgccataatccataagcacggcagcgactcggcaaacaaatatccccgtcatccttcaagttgcatgtgcgttggctgcgttcagtcatccgaatcacttaccagagtaagctcatcggaattccttatcttgccgccttcctgaaactcgaattatttagggtatagcgtatttgtttttcgaccagcgagatgaaataaatttaaatcgaattaaaaaaaccataataaagccgtaaatttacggtttatatcgatgtaaaagagtcacaccaagaaggaagttaaatacatttatattttgagtttagatcatacacacaacacaaacgcatggtcaatgcttatatagcaaagcaagtaaacagaacaactaaaacaaaaacacaataaaaacaaaagattaaattaaacttaagtaaaaatatccataaaaaaacagaataagtcattgagtttcaattgactgaaaaagaatatatttcgtgccttttaatttcgttaacaacgaaatacttaacccgctaacaaattaagg
Hcp gene cluster promoter (PECA4275) accggcaagtatcagcaataagtgtttctctcggtcacgattatctatcgccgctaaccggcgcagccttaacgagtgtaggacaacacgccagcgcatgcccaataggcgtatcccgttaagatcctgaccgagaatgacatacgcattatgccagttaatggcagcacttcactatgctgaaaaatgaatgatctcgtgtggcgggctccatagggaaaatcgcccatacttaatggcctatcaaaatcatcgagccgcactgacaaaccacttacgtaaaatcatgccaacgctagcttttacttttgttacccgaatgacgtcttttgctatgcaagatatgcatagcaaatataagtaattactcacttacatttttataaacgcgagcaaattatcgccagatatagctttttactatcgataaccaccgtttcgattggttattcatttcaataatttagtactaactcaaactttcagaataactgcgcattctgcgcaacttcttgctcactttctggtggaaatcactttttgcgcaatggatgccgttttttcgcgaggtctgcgcaattttttgctcaaattttgcttagggggaattttagttaagatatttatcattaaaaacaatcagatatgaattggcatacgaattgctatacagacggtgaagttactaaacccgttcaacaacaaggagcaacactatgccaactcc
Hcp gene cluster promoter (PECA2866) tgatattgataaatttatgaggatatcctccccattcatcgtcaataccttctaaatagacggacatctcacagttctaactcaaactttcagcataaccgcgcattctgcgcaacttcttgctcactttctggtggaaatcactttttgcgcaatggatgccgttttttcgcgaggtctgcgcaatttcttgctcaaatttgacttaggggaaatttgacttaagtaaattaacagcaaaaacaataacataaaatctggcatgcgaattgctatgcagagcgtgaagttactcatcacgttcaacaacaaggagcaagact

Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 1.4e-66 198..361

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-517 MQHALKLALALTRQHDETSLCDWLLETMQAAWQPQGMLLGMVDVSGRQLICQGRVHETPVALSLGVDDFSHPLAYALHKNQARTWDSLYGGARIEHREFRQMLVSVGTSCGLHALPLLSDSGKPLAVLALLDTPTRLQTLHQRGECERLAQVFCRQLMLLRELVHTRREQVALRDSLRQIKDEGQSRREQKKRVEATLIGRSVVIKELHQQIYQAAKHRLSVLIQGETGSGKDVVARLLHECSERVDKPFIAINCAAIPENLIESELFGYQKGAFSGAQSNKIGLVEQANGGTLFLDEVGDMPQSMQAKLLRVLETHSFRPLGAEKEVHSDFRLIAATHQPLEQQVSDGVFRQDLYHRLCQCLLLVAPLRERPDDIRLLCEHFIGQFADKPFSDERQKPIGPLSRAFLRQLVGYDFPGNVRELRNLLEVACAHTPSGEALSLTSLPPELRDRLTSSTAEEQDCYQHIHDLRIATQRYEAAVIEARLRQFQGNRLLVADSLNIPKRTLDHKCQKLEVN



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9878 - -



  Protein sequence of regulator: 518 a.a.    .

>REG0094 NC_004547:c3852298-3850745 [Pectobacterium atrosepticum SCRI1043]
MQHALKLALALTRQHDETSLCDWLLETMQAAWQPQGMLLGMVDVSGRQLICQGRVHETPVALSLGVDDFSHPLAYALHKN
QARTWDSLYGGARIEHREFRQMLVSVGTSCGLHALPLLSDSGKPLAVLALLDTPTRLQTLHQRGECERLAQVFCRQLMLL
RELVHTRREQVALRDSLRQIKDEGQSRREQKKRVEATLIGRSVVIKELHQQIYQAAKHRLSVLIQGETGSGKDVVARLLH
ECSERVDKPFIAINCAAIPENLIESELFGYQKGAFSGAQSNKIGLVEQANGGTLFLDEVGDMPQSMQAKLLRVLETHSFR
PLGAEKEVHSDFRLIAATHQPLEQQVSDGVFRQDLYHRLCQCLLLVAPLRERPDDIRLLCEHFIGQFADKPFSDERQKPI
GPLSRAFLRQLVGYDFPGNVRELRNLLEVACAHTPSGEALSLTSLPPELRDRLTSSTAEEQDCYQHIHDLRIATQRYEAA
VIEARLRQFQGNRLLVADSLNIPKRTLDHKCQKLEVN*

  Nucleotide sequence of regulator: 1554 bp    .

>REG0094 NC_004547:c3852298-3850745 [Pectobacterium atrosepticum SCRI1043]
ATGCAACATGCCCTCAAACTGGCGCTAGCACTCACCCGACAGCATGATGAAACTAGTCTGTGCGACTGGCTGCTGGAGAC
GATGCAGGCCGCCTGGCAGCCGCAAGGGATGCTGCTGGGGATGGTGGATGTCAGCGGCAGACAGCTGATCTGTCAGGGGC
GGGTGCATGAGACGCCGGTGGCGCTGAGTCTGGGGGTGGATGACTTTAGCCATCCGCTGGCTTATGCGCTGCATAAGAAT
CAGGCGCGTACCTGGGATTCTCTTTATGGCGGTGCTCGTATTGAGCACCGTGAATTTCGGCAGATGCTGGTCTCTGTCGG
GACGAGTTGCGGGCTTCACGCGTTGCCGCTGTTGTCGGACAGCGGTAAGCCGCTGGCGGTATTAGCGCTGTTGGATACGC
CGACGCGCCTGCAAACGCTGCATCAGCGAGGCGAATGCGAGCGGCTGGCTCAGGTATTTTGCCGCCAGCTTATGCTGCTG
CGTGAGCTGGTACACACCCGACGGGAACAGGTCGCCTTGAGAGATTCTCTCCGCCAGATTAAGGATGAAGGGCAGAGTCG
TCGCGAGCAGAAAAAGCGGGTGGAAGCCACCCTGATTGGCCGATCTGTCGTGATTAAAGAGCTGCATCAGCAGATTTATC
AGGCGGCAAAACACCGTCTTTCAGTGCTGATTCAGGGCGAAACCGGTTCAGGGAAAGATGTGGTGGCTCGCTTGCTTCAT
GAGTGTTCCGAACGCGTCGACAAACCCTTTATTGCTATCAACTGTGCCGCAATCCCCGAAAATCTGATTGAAAGCGAACT
GTTTGGCTATCAGAAAGGGGCATTCTCTGGCGCACAAAGTAACAAAATTGGTCTGGTTGAGCAGGCTAACGGCGGCACGC
TGTTTTTGGATGAAGTCGGCGATATGCCGCAGTCCATGCAAGCCAAGCTGTTAAGGGTATTGGAGACACATAGCTTCCGC
CCTCTGGGCGCGGAGAAAGAAGTTCATTCAGATTTCCGTCTGATTGCCGCGACGCACCAGCCGCTGGAACAGCAGGTGTC
TGACGGCGTGTTCCGGCAGGATCTCTATCACCGCCTCTGCCAGTGCCTGTTGCTGGTCGCGCCGCTGCGTGAGCGGCCAG
ATGATATTCGTCTGCTGTGCGAGCACTTCATCGGTCAATTTGCAGATAAACCATTTTCGGATGAACGACAAAAGCCCATA
GGGCCATTGAGTCGCGCTTTCCTGCGCCAGCTCGTGGGGTACGACTTTCCCGGCAATGTGCGGGAACTACGTAACCTGCT
GGAAGTGGCGTGTGCACATACGCCGAGCGGTGAAGCGCTGTCCTTGACATCGCTGCCGCCCGAACTGCGTGACCGGCTGA
CGAGCAGCACGGCTGAGGAACAGGATTGTTATCAGCATATCCACGATTTGCGTATAGCCACACAGCGGTATGAAGCGGCT
GTGATTGAGGCACGCCTGCGCCAGTTTCAGGGCAACCGCCTGCTGGTAGCCGATAGCCTCAATATCCCTAAACGCACTCT
TGACCACAAATGCCAGAAGCTGGAGGTGAATTGA